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| Lin,Juan; Zhou,Xuanwei; Fei,Jiong; Liao,Zhihua; Jin,Wang; Sun,Xiaofen; Tang,Kexuan. |
| A gene encoding a mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was isolated from leaves of Pinellia pedatisecta using genomic walker technology. The ppa contained an 1140-bp 5-upstream region, a 771-bp open reading frame (ORF) and an 829-bp 3-downstream region. The ORF encoded a precursor polypeptide of 256 amino acid residues with a 24-amino acid signal peptide. There were one putative TATA box and six possible CAAT boxes lying in the 5-upstream region of ppa. The ppa showed significant similarity at the nucleic acid level with genes encoding mannose-binding lectins from other Araceae species such as Pinellia ternata, Arisaema heterophyllum, Colocasia esculenta and Arum maculatum. At the amino acid level, PPA also shared varying... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genomic cloning; Genomic walker technology; Mannose-binding lectin; Pinellia pedatisecta; Pinellia pedatisecta agglutinin (PPA). |
| Ano: 2006 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100003 |
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