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| Registros recuperados: 72 | |
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| ARDISSON-ARAÚJO, D. M. P.; PEREIRA, B. T.; MELO, F. L.; RIBEIRO, B. M.; BÁO, S. N.; ZANOTTO, P. M. de A.; MOSCARDI, F.; KITAJIMA, E. W.; SOSA-GÓMEZ, D. R.; WOLFF, J. L. C.. |
| A betabaculovirus (DisaGV) was isolated from Diatraea saccharalis (Lepidoptera: Crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in Brazil. The complete genome sequence of DisaGV was determined using the 454-pyrosequencing method. The genome was 98,392 bp long, which makes it the smallest lepidopteran-infecting baculovirus sequenced to date. It had a G + C content of 29.7 % encoding 125 putative open reading frames (ORF). All the 37 baculovirus core genes and a set of 19 betabaculovirus-specific genes were found. A group of 13 putative genes was not found in any other baculovirus genome sequenced so far. A phylogenetic analysis indicated that DisaGV is a member of Betabaculovirus genus and that it is a sister... |
| Tipo: Separatas |
Palavras-chave: Cana.; Genoma.; Betabaculovirus; Lepidoptera.. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1059049 |
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| RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; TASSI, A. D.; CALEGARIO, R. F.; HARAKAVA, R.; NOME, C F.; KITAJIMA, E. W.; ASTUA, J. de F.. |
| An increasing number of plant species have been recognized or considered likely reservoirs of viruses transmitted by Brevipalpus mites. A tiny fraction of these viruses, primarily those causing severe economic burden to prominent crops, have been fully characterized. In this study, based on high-throughput sequencing, transmission electron microscopy analyses of virions in plant-infected tissues, viral transmission experiments, and the morphoanatomical identification of the involved Brevipalpus mites, we describe molecular and biological features of viruses representing three new tentative species of the family Kitaviridae. The genomes of Solanum violifolium ringspot virus (SvRSV, previously partially characterized), Ligustrum chlorotic spot virus... |
| Tipo: Artigo de periódico |
Palavras-chave: Vírus; Planta Ornamental; Brevipalpus; Virion; Ornamental plants. |
| Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148258 |
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| KUHN, G.; LIN, M. T.; KITAJIMA, E. W.. |
| O vírus do mosaico do picão (VMP) foi inativado no extrato foliar mantido entre 55_60ºC por 10 minutos e nas diluições acima de 10-3 ou ainda quando conservado após 5 e 21 dias a 24-26oC e a 4-50C, respectivamente. Nos exames ao microscópio eletrônico de preparações "leaf dip" sempre foram detectadas partículas alongadas e flexíveis, com comprimento normal de ca. 735 nm e, esporadicamente, formações lamelares. Em secções ultrafinas verificou- se a presença de inclusões citoplasmáticas lamelares do tipo catavento. Essas inclusões puderam ser observadas em células epidermais quando examinadas no microscópio de luz. O VMP foi parcialmente purificado pela clanficação do suco com n-butanol e pela precipitação do vírus por centrifugação em gradiente de densidade... |
| Tipo: Artigo de periódico |
Palavras-chave: Picão; Vírus do Mosaico do picão; Caracterização; Doença de Planta; Vírus. |
| Ano: 1982 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/536828 |
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| ALMEIDA, A. M. R.; SAKAI, J.; OLIVEIRA, T. G. O.; BELINTANI, P.; KITAJIMA, E. W.; SOUTO, E. R.; NOVAES, T. G. de; NORA, P. S.. |
| A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular... |
| Tipo: Separatas |
Palavras-chave: Soja.. |
| Ano: 2005 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/468641 |
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| FERREIRA, P. de T. O.; LOCALI-FABRIS, E. C.; FREITAS-ÁSTUA, J.; ANTONIOLI-LUIZON, R.; GOMES, R. T.; MACHADO, M. A.; KITAJIMA, E. W.. |
| Solano-violeta (Solanum violaefolium) é uma planta ornamental rasteira usada para cobrir solos de áreas sombreadas. Um vírus que induz manchas anelares nas folhas desta planta, tentativamente designado Solanum violaefolium ringspot virus - SvRSV, transmitido pelo ácaro Brevipalpus phoenicis (Acari: Tenuipalpidae) foi encontrado em Piracicaba, SP. Trata-se de um vírus baciliforme que se assemelha a outros vírus do tipo citoplasmático transmitidos por Brevipalpus sp. Este trabalho teve como objetivo relatar propriedades biológicas e estabelecer uma caracterização molecular parcial do SvRSV. O vírus pode ser transmitido mecanicamente a várias outras espécies botânicas, causando lesões localizadas. Entre as espécies avaliadas, Datura stramonium mostrou-se a... |
| Tipo: Artigo de periódico |
Palavras-chave: SvRSV; CiLV-C; DsRNA; Sequenciamento; Efeito citopático. |
| Ano: 2007 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/654616 |
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| OLIVEIRA, A. S. de; MELO, F. L.; INOUE NAGATA, A. K.; NAGATA, T.; KITAJIMA, E. W.; RESENDE, R. O.. |
| Tospoviruses (Genus Tospovirus, Family Bunyaviridae) are phytopathogens responsible for significant worldwide crop losses. They have a tripartite negative and ambisense RNA genome segments, termed S (Small), M (Medium) and L (Large) RNA. The vector-transmission is mediated by thrips in a circulative-propagative manner. For new tospovirus species acceptance, several analyses are needed, e.g., the determination of the viral protein sequences for enlightenment of their evolutionary history. |
| Tipo: Separatas |
Palavras-chave: Análise serológica; Sequencia genômica; Bean necrotic mosaic virus; SVNaV; BeNMV; Virus; Genoma; Linhagem; Sintoma; Tospovirus. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/934717 |
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| TASSI, A. D.; RAMOS-GONZÁLEZ, P. L.; SINICO, T. E.; KITAJIMA, E. W.; ASTUA, J. de F.. |
| Plant viruses transmitted by mites of the genus Brevipalpus are members of the genera Cilevirus, family Kitaviridae, or Dichorhavirus, family Rhabdoviridae. They produce non-systemic infections that typically display necrotic and/or chlorotic lesions around the inoculation loci. The cilevirus citrus leprosis virus C (CiLV-C) causes citrus leprosis, rated as one of the most destructive diseases affecting this crop in the Americas. CiLV-C is vectored in a persistent manner by the flat mite Brevipalpus yothersi. Upon the ingestion of viral particles with the content of the infected plant cell, virions must pass through the midgut epithelium and the anterior podocephalic gland of the mites. Following the duct from this gland, virions reach the salivary canal... |
| Tipo: Artigo de periódico |
Palavras-chave: Vírus; Fruta Cítrica; Citrus leprosis virus C. |
| Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148259 |
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| EIRAS, M.; TARGON, M. L. P. N.; FAJARDO, T. V. M.; FLORES, R.; KITAJIMA, E. W.. |
| Viroids, non-protein-coding small (246-40 I nt) eircular single-stranded RNAs with autonornous replication, are currently classified into two farnilics. Within the farnily Pospiviroidae, Citrus exocortis viroid (CEVd) belongs to lhe genus Pospiviroid while Hop stunt viroid (HSVd) is the single rnembcr of the genus Hostuviroid. These pathogens are distributcd worldwide and infect a large nurnbcr of hosts. In Brazil, isolates of CEVd and HSVd have been detected in both citrus and grapevine. To characterize and study the genetic variability of thcse viroids, total RNA frorn leaves of grapevine Vitis vinifera 'Cabemet Sauvignon' and V Iabrusca 'Niagara Rosada' frorn Bento Gonçalves, RS, was used as a tcmplate for RTPCR amplification with specific primers for... |
| Tipo: Artigo de periódico |
Palavras-chave: CEVd; HSVd; Doença de Planta; Uva; Vírus; Viticultura; Hostuviroid; Pospiviroid; Pospiviroidae. |
| Ano: 2006 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/541733 |
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| Registros recuperados: 72 | |
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