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| Registros recuperados: 57 | |
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| ARDISSON-ARAÚJO, D. M. P.; PEREIRA, B. T.; MELO, F. L.; RIBEIRO, B. M.; BÁO, S. N.; ZANOTTO, P. M. de A.; MOSCARDI, F.; KITAJIMA, E. W.; SOSA-GÓMEZ, D. R.; WOLFF, J. L. C.. |
| A betabaculovirus (DisaGV) was isolated from Diatraea saccharalis (Lepidoptera: Crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in Brazil. The complete genome sequence of DisaGV was determined using the 454-pyrosequencing method. The genome was 98,392 bp long, which makes it the smallest lepidopteran-infecting baculovirus sequenced to date. It had a G + C content of 29.7 % encoding 125 putative open reading frames (ORF). All the 37 baculovirus core genes and a set of 19 betabaculovirus-specific genes were found. A group of 13 putative genes was not found in any other baculovirus genome sequenced so far. A phylogenetic analysis indicated that DisaGV is a member of Betabaculovirus genus and that it is a sister... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Betabaculovirus; Lepidoptera; Cana; Genoma. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1059049 |
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| KUHN, G.; LIN, M. T.; KITAJIMA, E. W.. |
| O vírus do mosaico do picão (VMP) foi inativado no extrato foliar mantido entre 55_60ºC por 10 minutos e nas diluições acima de 10-3 ou ainda quando conservado após 5 e 21 dias a 24-26oC e a 4-50C, respectivamente. Nos exames ao microscópio eletrônico de preparações "leaf dip" sempre foram detectadas partículas alongadas e flexíveis, com comprimento normal de ca. 735 nm e, esporadicamente, formações lamelares. Em secções ultrafinas verificou- se a presença de inclusões citoplasmáticas lamelares do tipo catavento. Essas inclusões puderam ser observadas em células epidermais quando examinadas no microscópio de luz. O VMP foi parcialmente purificado pela clanficação do suco com n-butanol e pela precipitação do vírus por centrifugação em gradiente de densidade... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Picão; Vírus do Mosaico do picão; Caracterização; Doença de Planta; Vírus. |
| Ano: 1982 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/536828 |
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| ALMEIDA, A. M. R.; SAKAI, J.; OLIVEIRA, T. G. O.; BELINTANI, P.; KITAJIMA, E. W.; SOUTO, E. R.; NOVAES, T. G. de; NORA, P. S.. |
| A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Soja. |
| Ano: 2005 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/468641 |
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| EIRAS, M.; TARGON, M. L. P. N.; FAJARDO, T. V. M.; FLORES, R.; KITAJIMA, E. W.. |
| Viroids, non-protein-coding small (246-40 I nt) eircular single-stranded RNAs with autonornous replication, are currently classified into two farnilics. Within the farnily Pospiviroidae, Citrus exocortis viroid (CEVd) belongs to lhe genus Pospiviroid while Hop stunt viroid (HSVd) is the single rnembcr of the genus Hostuviroid. These pathogens are distributcd worldwide and infect a large nurnbcr of hosts. In Brazil, isolates of CEVd and HSVd have been detected in both citrus and grapevine. To characterize and study the genetic variability of thcse viroids, total RNA frorn leaves of grapevine Vitis vinifera 'Cabemet Sauvignon' and V Iabrusca 'Niagara Rosada' frorn Bento Gonçalves, RS, was used as a tcmplate for RTPCR amplification with specific primers for... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: CEVd; HSVd; Doença de Planta; Uva; Vírus; Viticultura; Hostuviroid; Pospiviroid; Pospiviroidae. |
| Ano: 2006 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/541733 |
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| ALMEIDA, A. M. R.; PIUGA, F. F.; MARIN, S. R. R.; KITAJIMA, E. W.; GASPAR, J. O.; OLIVEIRA, T. G. de; MORAES, T. G. de.. |
| Stunting and stem necrosis were noticed in soybeans (Glycine max) grown in 2000/2001 in West Central Brazil the same condition was also observed in the following year in plantations as far as 2,000 km from the initial area. Based on transmission (mechanical, graft, insect vector), purification and serology, electron microscopy and molecular studies the causal agent was determined to be a whitefly-borne carlavirus which is possibly related to Cowpea mild mottle virus (CpMMV). Additional keywords: Cowpea mild mottle virus, whitefly, Bemisia tabaci biotype B. |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Doença de planta; Soja; Vírus. |
| Ano: 2005 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/467946 |
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| ANTONIOLI-LUIZON, R.; BARBOSA, C. de J.; LARANJEIRA, F. F.; KITAJIMA, E. W.; FREITAS-ASTÚA, J.. |
| Maracujá (do tupi mara Kuya, "fruto que serve" ou "alimento na cuia") é a denominação dada ao fruto produzido por plantas do gênero Passiflora da família Passifloraceae. Das cerca de 600 espécies de Passiflora, P. edulis f. flavicarpa (maracujá amarelo), P. edulis (maracujá roxo) e P. alata (maracujá doce) são responsáveis por 95% da área plantada no Brasil, o maior produtor mundial da fruta com 684.376 toneladas produzidas em 2008 (IBGE, 2009). Dentre os Estados brasileiros, o destaque é a Bahia, principal produtor com 275.445 t produzidas em 2008, seguido por Espírito Santo, Sergipe, Minas Gerais e São Paulo (IBGE,2009). |
| Tipo: Comunicado Técnico (INFOTECA-E) |
Palavras-chave: Brevipalpus Phoenicis; Doença de Planta; Maracujá.; Passiflora.. |
| Ano: 2009 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/711786 |
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| LEASTRO, M. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á.. |
| Brevipalpus-transmitted viruses (BTVs) belong to the genera Dichorhavirus and Cilevirus and are the main causal agents of the citrus leprosis (CL) disease. In this report, we explored aspects related to the movement mechanism mediated by dichorhaviruses movement proteins (MPs) and the homologous and heterologous interactions among viral proteins related to the movement of citrus leprosis-associated viruses. The membrane-spanning property and topology analysis of the nucleocapsid (N) and MP proteins from two dichorhaviruses revealed that the MPs are proteins tightly associated with the cell membrane, exposing their N- and C-termini to the cytoplasm and the inner part of the nucleus, whereas the N proteins are not membrane-associated. Subcellular... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Vírus. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126324 |
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| Registros recuperados: 57 | |
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