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Detection of adrenocortical autoantibodies in Addison's disease with a peroxidase-labelled protein A technique 56
Silva,R.C.; Faiçal,S.; Laureti,S.; Falorni,A.; Dib,S.A.; Kater,C.E..
Adrenocortical autoantibodies (ACA), present in 60-80% of patients with idiopathic Addison's disease, are conventionally detected by indirect immunofluorescence (IIF) on frozen sections of adrenal glands. The large-scale use of IIF is limited in part by the need for a fluorescence microscope and the fact that histological sections cannot be stored for long periods of time. To circumvent these restrictions we developed a novel peroxidase-labelled protein A (PLPA) technique for the detection of ACA in patients with Addison's disease and compared the results with those obtained with the classical IIF assay. We studied serum samples from 90 healthy control subjects and 22 patients with Addison's disease, who had been clinically classified into two groups:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Autoantibodies detection; Addison's disease; Peroxidase-labelled protein A; Indirect immunofluorescence; Adrenal gland.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900007
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