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Endogenous regulation of 11-deoxycorticosterone (DOC) and corticosteroid receptors (CRs) during rainbow trout early development and the effects of corticosteroids on hatching. ArchiMer
Kiilerich, Pia; Geffroy, Benjamin; Valotaire, Claudiane; Prunet, Patrick.
Clear evidence for a physiological role of the mineralocorticoid-like hormone 11-deoxycorticosterone (DOC) and the mineralocorticoid receptor (MR) in fish is still lacking. Efforts to demonstrate an osmoregulatory role for this hormone has so far not been conclusive, while a few scattered studies have indicated a role for DOC in development and reproduction. In this study, we investigate the onset of de novo DOC synthesis in parallel with endogenous corticosteroid receptor mRNA production from fertilization to the swim-up stage in rainbow trout. Whole egg DOC content decreased from fertilization until hatching followed by an increase to pre-fertilization levels just after hatching. Onset of de novo transcription of corticosteroid receptor mRNA’s was...
Tipo: Text Palavras-chave: 11-Deoxycortieosterone; Cortisol; Mineralocorticoid receptor; Glucocorticoid receptor; Fish; Development.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00443/55506/57055.pdf
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Regulation of the corticosteroid signalling system in rainbow trout HPI axis during confinement stress. ArchiMer
Kiilerich, Pia; Servili, Arianna; Peron, Sandrine; Valotaire, Claudiane; Goardon, Lionel; Leguen, Isabelle; Prunet, Patrick.
This study aims to shed light on corticosteroid regulation of stress in teleost fish with focus on the corticosteroid signalling system. The role of the mineralocorticoid-like hormone 11-deoxycorticosterone (DOC) in fish is still enigmatic, as is the function of the mineralocorticoid receptor, MR. Low plasma DOC levels and ubiquitous tissue distribution of MR question the physiological relevance of the mineralocorticoid-axis. Furthermore, the particular purpose of each of the three corticosteroid receptors in fish, the glucocorticoid receptors, GR1 and GR2, and the MR, is still largely unknown. Therefore we investigate the regulation of cortisol and DOC in plasma and mRNA levels of MR, GR1 and GR2 in the HPI-axis tissue (hypothalamus, pituitary and...
Tipo: Text Palavras-chave: Stress; Rainbow trout; Confinement; Cortisol; DOC; MR; GR1; GR2; HPI-axis.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00396/50737/51439.pdf
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