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Registros recuperados: 9
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Croissance de la racine de peuplier en réponse au stress osmotique : approche moléculaire dans la zone de croissance Inra
Hummel, A.; Rinaldi, C.; Kohler, A.; Duplessis, S.; Merret, R.; Dreyer, E.; Bogeat, M.B..
Tipo: Meeting Paper Palavras-chave: STRESS OSMOTIQUE; DEFICIT HYDRIQUE; CULTURE HYDROPONIQUE.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20099855d7d5&uri=/notices/prodinra1/2009/07/
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Development and validation of an oligonucleotide microarray to characterise ectomycorrhizal fungal communities Inra
Reich, M.; Kohler, A.; Martin, F.; Buee, M..
Background: In forest ecosystems, communities of ectomycorrhizal fungi (ECM) are influenced by several biotic and abiotic factors. To understand their underlying dynamics, ECM communities have been surveyed with ribosomal DNA-based sequencing methods. However, most identification methods are both time-consuming and limited by the number of samples that can be treated in a realistic time frame. As a result of ongoing implementation, the array technique has gained throughput capacity in terms of the number of samples and the capacity for parallel identification of several species. Thus far, although phylochips (microarrays that are used to detect species) have been mostly developed to trace bacterial communities or groups of specific fungi, no phylochip has...
Tipo: Journal Article Palavras-chave: FINE-SCALE DISTRIBUTION; IDENTIFICATION; FOREST; ARRAY; HOST; SOIL; BASIDIOMYCOTA; SPECIFICITY; DIVERSITY; PATHOGENS.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010b686b787&uri=/notices/prodinra1/2010/10/
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Effet du stress osmotique sur la croissance racinaire du peuplier : approche par génomique fonctionnelle à l'échelle de la zone de croissance Inra
Bogeat, M.B.; Hummel, A.; Duplessis, S.; Kohler, A..
Tipo: Meeting Paper Palavras-chave: STRESS OSMOTIQUE; POPULUS DELTOÏDES X NIGRA CV LAMBRO.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20098638dbb0&uri=/notices/prodinra1/2010/11/
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Fungi have three tetraspanin families with distinct functions Inra
Lambou, K; Tharreau, D.; Kohler, A.; Sirven, C; Marguerettaz, M; Barbisan, C; Sexton, A; Kellner, E; Martin, F.; Howlett, B; Orbach, M; Lebrun, MH.
Background: Tetraspanins are small membrane proteins that belong to a superfamily encompassing 33 members in human andmouse. These proteins act as organizers of membrane-signalling complexes. So far only two tetraspanin families have beenidentified in fungi. These are Pls1, which is required for pathogenicity of the plant pathogenic ascomycetes, Magnaporthe grisea,Botrytis cinerea and Colletotrichum lindemuthianum, and Tsp2, whose function is unknown. In this report, we describe a third familyof tetraspanins (Tsp3) and a new family of tetraspanin-like proteins (Tpl1) in fungi. We also describe expression of some of thesegenes in M. grisea and a basidiomycete, Laccaria bicolor, and also their functional analysis in M. grisea. Results: The exhaustive search...
Tipo: Journal Article Palavras-chave: BASIDIOMYCETES; ASCOMYCETES; COPRINUS CINEREUS; LACCARIA BICOLOR; MAGNAPORTHE GRISEA; PROTEINE MEMBRANAIRE; TETRASPANINE; PATHOGENIE; POUVOIR PATHOGENE; INFECTION RICE; BARLEY; GENE EXPRESSION; MYCELIUM; PATHOGENICITY; PLANT PATHOGENIC FUNGI; SURFACE PROTEINS; TRANSCRIPTION; DNA TRANSCRIPTION; MEMBRANE PROTEINS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20101f8edf6&uri=/notices/prodinra1/2010/11/
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Genome-wide identification of NBS resistance genes in Populus trichocarpa Inra
Kohler, A.; Rinaldi, C.; Duplessis, S.; Baucher, M.; Geelen, D.; Duchaussoy, F.; Meyers, BC; Boerjan, W.; Martin, F..
As the largest class of disease resistance R genes, the genes encoding nucleotide binding site and leucine-rich repeat proteins (“NBS-LRR genes”) play a critical role in defending plants from a multitude of pathogens and pests. The diversity of NBS-LRR genes was examined in the Populus trichocarpa draft genome sequence. The NBS class of genes in this perennial tree is large and diverse, comprised of ∼400 genes, at least twice the complement of Arabidopsis. The NBS family can be divided into multiple subfamilies with distinct domain organizations. It includes 119 Coiled-Coil-NBS-LRR genes, 64 TIR-NBS-LRR genes, 34 BED-finger-NBS-LRR, and both truncated and unusual NBS- and NBS-LRR-containing genes. The transcripts of only 34 NBS-LRR genes were detected in...
Tipo: Journal Article Palavras-chave: Populus; NBS-LRR; Resistance.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010b08a4238&uri=/notices/prodinra1/2010/10/
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Identification of genes differentially expressed in extraradical mycelium and ectomycorrhizal roots during Paxillus involutus-Betula pendula ectomycorrhizal symbiosis Inra
Morel, M.; Jacob, C.; Kohler, A.; Johansson, T.; Martin, F.; Chalot, M.; Brun, A..
The development of ectomycorrhizal symbiosis leads to drastic changes in gene expression in both partners. However, little is known about the spatial regulation of symbiosis-regulated genes. Using cDNA array profiling, we compared the levels of expression of fungal genes corresponding to approximately 1,200 expressed sequenced tags in the ectomycorrhizal root tips (ECM) and the connected extraradical mycelium (EM) for the Paxillus involutus-Betula pendula ectomycorrhizal association grown on peat in a microcosm system. Sixty-five unique genes were found to be differentially expressed in these two fungal compartments. In ECM, a gene coding for a putative phosphatidylserine decarboxylase (Psd) was up-regulated by 24-fold, while genes coding for urea (Dur3)...
Tipo: Journal Article Palavras-chave: EXPRESSION DES GENES; ECTOMYCORHIZE; MYCELIUM; SYMBIOSE ECTOMYCORHIZIENNE; PUCE A ADN PAXILLUS INVOLUTUS; BETULA PENDULA.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007b15a99dd&uri=/notices/prodinra1/2007/07/
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Obligate biotrophpy features unraveled by the genomic analysis of rust fungi Inra
Duplessis, S.; Cuomo, CA; Lin, YC; Aerts, A.; Tisserant, E.; Veneault-Fourrey, C.; Joly, DL; Hacquard, S.; Amselem, J.; Cantarel, B.l; Chin, R.; Coutinho, P.M.; Feau, N.; Field, M.; Frey, P.; Gelhaye, É.; Goldberg, J.; Grabherr, M.G.; Kodira, C.D.; Kohler, A.; Kues, U.; Lindquist, E.A.; Lucas, M; Mago, R.; Mauceli, E.; Morin, E.; Murat, C.; Pangilinan, J.L.; Park, R.; Pearson, M.; Quesneville, H.; Rouhier, N.; Sakthikumar, S.; Salamov, A.A.; Schmutz, J.; Selles, B.; Shapiro, H.; Tanguay, P.; Tuskan, G.A.; Henrissat, B.; Van de Peer, Y.; Rouze, P.; Ellis, J.G.; Dodds, P.N.; Schein, J.E.; Zhong, S.; Hamelin, R.C.; Grigoriev, I.V.; Szabo, L.J.; Martin, F..
Rust fungi are some of the most devastating pathogens of crop plants. They are obligate biotrophs, which extract nutrients only from living plant tissues and cannot grow apart from their hosts. Their lifestyle has slowed the dissection of molecular mechanisms underlying host invasion and avoidance or suppression of plant innate immunity. We sequenced the 101-Mb genome of Melampsora larici-populina, the causal agent of poplar leaf rust, and the 89-Mb genome of Puccinia graminis f. sp. tritici, the causal agent of wheat and barley stem rust. We then compared the 16,399 predicted proteins of M. larici-populina with the 17,773 predicted proteins of P. graminis f. sp tritici. Genomic features related to their obligate biotrophic lifestyle include expanded...
Tipo: Journal Article Palavras-chave: COMPARATIVE GENOMICS; PLANT PATHOGEN; BASIDIOMYCETE; EVOLUTION; RUST EVOLUTION.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20117dba1845&uri=/notices/prodinra1/2011/06/
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Physiological and transcriptional responses of poplar to water deficit Inra
Kohler, A.; Bogeat, M.B.; Delaruelle, C.; Dreyer, E.; Guehl, J.M.; Martin, F..
The response of Populus trichocarpa x deltoides transcriptome to water-deficit stress wasassessed using experimental conditions that closely approximate stress development in the field.For dehydration treatment, 2-month-old cutting plantlets were grown for 19 days under restrictedirrigation to reach 35% of the transpiration rate of fully watered plants. At this stage, leaf waterpotential and shoot growth were not affected. In contrast, stomatal conductance andphotosynthesis of the fast growing leaf F3 were reduced by 90 and 60%, respectively. Osmoticadjustment was detected in this leaf – full turgor osmotic pressure was 20% higher than in controlplant –. Root growth was stimulated although relative water content was decreased. No osmoticadjustment was...
Tipo: Meeting Paper Palavras-chave: POPULUS; STRESS HYDRIQUE; SECHERESSE; DEFICIT HYDRIQUE; PHYSIOLOGIE; TRANSCRIPTOME; PROTEOME; POPULUS TRICHOCARPA X DELTOIDES CV BEAUPRE.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009269881a2&uri=/notices/prodinra1/2010/11/
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Transcript Profiling of Poplar Leaves upon Infection with Compatible and Incompatible Strains of the Foliar Rust Melampsora larici-populina Inra
Rinaldi, C.; Kohler, A.; Frey, P.; Duchaussoy, F; Ningre, F; Couloux, A.; Wincker, P; Le Thiec, D.; Fluch, S.; Martin, F.; Duplessis, S..
To understand key processes governing defense mechanisms in poplar (Populus spp.) upon infection with the rust fungus Melampsora larici-populina, we used combined histological and molecular techniques to describe the infection of Populus trichocarpa X Populus deltoides 'Beaupre' leaves by compatible and incompatible fungal strains. Striking differences in host-tissue infection were observed after 48-h postinoculation (hpi) between compatible and incompatible interactions. No reactive oxygen species production could be detected at infection sites, while a strong accumulation of monolignols occurred in the incompatible interaction after 48 hpi, indicating a late plant response once the fungus already penetrated host cells to form haustorial infection...
Tipo: Journal Article Palavras-chave: OBLIGATE BIOTROPHIC PATHOGEN; EXPRESSED SEQUENCE TAGS; FOR-GENE SPECIFICITY; HYPERSENSITIVE RESPONSE; HYBRID POPLAR; DISEASE RESISTANCE; LEAF RUST; FLAX RUST; AVIRULENCE GENES; GENOMIC SEQUENCE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20072887511d&uri=/notices/prodinra1/2011/04/
Registros recuperados: 9
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