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Registros recuperados: 15
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Alfalfa-Verticillium Albo-atrum Interactions : In Vitro Responses of Alfalfa Callus to Fungal Culture Filtrate and Cell Wall Components OAK
Koike, Masanori; Nanbu, Kouhei; Shimada, Tohru.
日本草地学会Journal of Japanese Society of Grassland Science 37(4):412-419(1992)より転載
Palavras-chave: Verticillium albo-atrum; Alfalfa; Callus; Cell wall components Culture filtrates; Disease resistance.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1772
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Alfalfa-Verticillium albo-atrum Interactions : IV. Reactions of alfalfa protoplasts to fungal culture filtrates and cell wall components OAK
Koike, Masanori; Simada, Tohru; Amemiya, Yoshimiki.
日本草地学会Journal of Japanese Society of Grassland Science 39(1):101-107(1993)より転載
Palavras-chave: Verticillium; Alfalfa; Cell wall components; Protoplast; Aibo-atrum; Culture filtrate.
Ano: 1993 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1771
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Biological control of Verticillium black spot of Japanese radish using Bacillus spp. and genotypic differentiation of selected antifungal Bacillus strains with antibiotic marker OAK
Kawai, Ayako; Kusunoki, Kaori; Aiuchi, Daigo; Koike, Masanori; Tani, Masayuki; Kuramochi, Katsuhisa; 川井, 彩子; 楠木, 芳; 相内, 大吾; 小池, 正徳; 谷, 昌幸; 倉持, 勝久.
Bacillus species can form endospores and have broad-spectrum activity of their antibiotics. For that reason, they offer several advantages over other bacteria for protection against root pathogens. This work was intended to determine the ability of Bacillus spp. to inhibit Verticillium dahliae. First, antagonistic activities of Bacillus spp. were studied in greenhouse trials. These results suggest that the bacterium can control soil-borne diseases. Secondly, to investigate the situation in a culture environment, Bacillus spp. were examined for their activity to control Verticillium black spot of Japanese radish and plant-growth promotion rhizobacteria (PGPR) effects in a field. After 2 months, these bacteria reduced Verticillium black spot of Japanese...
Palavras-chave: Bacillus spp.; Biological control; Verticillium black spot of Japanese radish; Verticillium dahliae.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/97
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Effects of fungal culture filtrates of Verticillium lecanii (Lecanicillium spp.) hybrid strains on Heterodera glycines eggs and juveniles OAK
Shinya, Ryoji; Aiuchi, Daigo; Kushida, Atsuhiko; Tani, Masayuki; Kuramochi, Katsuhisa; Koike, Masanori.
Received 21 May 2007; revised 19 November 2007; accepted 20 November 2007. Available online 29 November 2007.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1665
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Effects of land use and long-term organic matter application on low-molecular-weight organic acids in an Andisol OAK
Takata, Yusuke; Tani, Masayuki; Kato, Taku; Koike, Masanori; 谷, 昌幸; 加藤, 拓; 小池, 正徳.
http://www.academicjournals.org/jssem/abstracts/abstracts/abstracts2011/25%20October/Takata%20et%20al.htm
Palavras-chave: Land use; Long-term organic matter application; Low-molecular-weight organic acids; Malic acid; Water-extractable organic carbon.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3578
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Effects of phytotoxic metabolites from Verticillium dahliae and ouabain on tissues and callus cultures from susceptible and resistant cultivars of tomato OAK
Murakami, Takanori; Koike, Masanori; Shimada, Tohru; 村上, 隆紀; 小池, 正徳; 嶋田, 徹.
Palavras-chave: Tomato; Verticillium dahliae; トマト.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1345
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In vitro におけるジャガイモ半身萎ちょう病抵抗性の評価 OAK
Koike, Masanori; Kozawa, Tohru; Okino, Kaori; Nagao, Hideyuki; Shimada, Tohru; 小池, 正徳; 小沢, 徹; 沖野, 香織; 長尾, 英幸; 嶋田, 徹.
Palavras-chave: Potato; Verticillium albo-atrum; ジャガイモ.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1342
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Low-molecular-weight aliphatic carboxylic acids in soils incubated with decomposing forest litter OAK
Tani, Masayuki; Koike, Masanori; Kuramochi, Katsuhisa; Higashi, Teruo; 谷, 昌幸; 小池, 正徳; 倉持, 勝久; 東, 照雄.
The effects of the addition of decomposing forest litter, collected from Podzolic soil organic layers (Oi, Oe and Oa) and Brown Forest soil Oe layers, on the amounts and composition of low molecular weight aliphatic carboxylic acids (LACAs) in soils were investigated by incubation experiments for 4 weeks. The total amounts of LACAs were greater at the beginning of incubation (384 to 1155 μmol kg-1), but decreased abruptly after 1 week, becoming relatively stable for a period of 1 to 4 weeks (299 to 377 μmol kg-1). The average amounts (without 0 weeks) of LACAs, especially acetic acid and citric acid, in soils incubated with forest litter under moderately moist conditions, were smaller than in the blank soil. Larger amounts of LACAs, especially formic acid,...
Palavras-chave: Decomposing forest litter; Forest soils; Incubation experiment; Low-molecular-weight aliphatic carboxylicacid (LACA); Soil microflora.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/98
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Pre-inoculation of cucumber roots with Verticillium lecanii (Lecanicillium muscarium) induces resistance to powdery mildew OAK
Hirano, Emiko; Koike, Masanori; Aiuchi, Daigo; Tani, Masayuki; 平野, 恵美子; 小池, 正徳; 相内, 大吾; 谷, 昌幸.
The ability of Verticillium lecanii (Lecanicillium muscarium; B-2) to induce host resistance and defense responses against subsequent challenge with cucumber powdery mildews was examined in a glasshouse. A root test showed that, after inoculation with V. lecanii blastspore on cucumber roots, induction of systemic resistance in those V. lecanii pre-inoculated plants engendered significantly fewer lesions and reduced disease severity compared with non-inoculated plants. Furthermore, in plants inoculated with V. lecanii blastspore on their roots, the fungi showed high colonizing ability on cucumber rhizoplane and inside root tissues. Nevertheless, activities of peroxidase (PO) and phenylalanine ammonia-lyase (PAL) increased slightly, but not significantly. In...
Palavras-chave: Induced resistance; Lecanicillium muscarium; Powdery Mildew; Verticillium lecanii.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2277
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アルファルファとVerticillium albo-atrumの相互作用 : 6. 菌体細胞壁成分に対して高いPAL活性を示す細胞系統の選抜とその特性 OAK
Koike, Masanori; Katsumata, Yukiyoshi; Amemiya, Yoshimiki; Shimada, Tohru.
To obtain alfalfa cell lines with high phenylalanine ammonia-lyase (PAL) activity in response to heat-released cell wall components (elicitor) of an alfalfa pathogen (Verticillium albo-atrum), in vitro selection was performed. The selection using a replica plating methods was carried out on 65 cell lines derived from suspension cultures treated and/or non-treated with N-metyl-N'-nitro-N-nitrosoguanidine (MNNG) of the susceptible genotype of cv. Vertus. Three stable high PAL cell lines in response to elicitor (two cell lines recovered from MNNG treatment, one from non-MNNG treatment) were isolated. All these cell lines showed higher PAL activities, lignin depositions and phytoalexin accumulations than the parental cell line after treatment with active V....
Palavras-chave: Alfalfa; In vitro selection; Phenylalanine ammonia-lyase; アルファルファ; 細胞選抜; Verticillium albo-atrum; フェニルアラニンアンモニアリアーゼ.
Ano: 1995 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1770
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ダイズシストセンチュウに対するVerticillium lecanii プロトプラスト融合株の微生物防除資材としての可能性:プロトプラスト融合は植物寄生性線虫の生物的防除において有用な手法と成り得るか? OAK
Shinya, Ryoji; Watanabe, Ai; Aiuchi, Daigo; Tani, Masayuki; Kuramochi, Katsuhisa; Kushida, Atsuhiko; Koike, Masanori; 新屋, 良治; 渡部, 愛; 相内, 大吾; 谷, 昌幸; 倉持, 勝久; 串田, 篤彦; 小池, 正徳.
Verticillium lecanii is a potentially useful biological control agent (BCA) for soybean cyst nematode (SCN), Heterodera glycines. The objective of this study was to screen hybrid strains of V. lecanii, derived from protoplast fusion, for effective control agents of SCN, and to investigate whether the protoplast fusion technique is an effective tool for development of nematode control agents. Three parental strains (Vertalec®, Mycotal®, and B-2) and their 162 hybrid strains were screened in greenhouse pot tests. Some of these hybrid strains suppressed damage on soybean plants and reduced the density of SCN in the soil. In particular, one hybrid strain, AaF42, was observed to reduce the nematode egg density by 93.2% as compared with the control. Furthermore,...
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4162
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トレント大学(カナダ)におけるCommunity-based Research(CBR) OAK
小池, 正徳; 谷, 昌幸; 三浦, 秀穂; 倉持, 勝久; 佐々木, 市夫; Koike, Masanori; Tani, Masayuki; Miura, Hideho; Kuramochi, Katsuhisa; Sasaki, Ichio.
実践報告
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1761
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帯広畜産大学における全学農畜産実習 OAK
小池, 正徳; 谷, 昌幸; 三浦, 秀穂; 倉持, 勝久; 三好, 俊三; 渡邊, 芳之; 石橋, 憲一; Koike, Masanori; Tani, Masayuki; Miura, Hideho; Kuramochi, Katsuhisa; Miyoshi, Syunzou; Watanabe, Yoshiyuki; Ishibashi, Kenichi.
実践報告
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1760
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昆虫寄生菌Verticillium lecanii (Lecanicillium spp.) によるダイコンバーティシリウム黒点病の生物防除 OAK
楠木, 芳; 川井, 彩子; 相内, 大吾; 小池, 正徳; 谷, 昌幸; 倉持, 勝久; Kusunoki, Kaori; Kawai, Ayako; Aiuchi, Daigo; Koike, Masanori; Tani, Masayuki; Kuramochi, Katsuhisa.
ダイコンバーティシリウム黒点病に対する防除効果を昆虫寄生菌Verticillium lecaniiを用いて検討した。温室試験と圃場試験でV. lecaniiの7菌株(A-2、B-2、C-1、2aF30、2aF31、2aF43、AaF101)を用いて実施した。V. lecaniiの分生胞子を処理したポット試験では全てのV. lecanii菌株で高い防除効果があり、病原菌単独処理と比較して有意な差が認められた。なかでもB-2が最も高い防除効果を示し、病原菌単独処理の発病率51.2%と比較して31.4%となった。V. lecaniiのフスマ培養土を用いたポット試験における病徴抑制効果は病原菌単独処理区との有意な差が認められたのはC-1処理区のみであった。A-2、B-2処理区においても抑制効果の傾向がみられたが、生長促進効果では顕著な差はみられなかった。また、圃場試験では全てのV. lecanii処理区で発病が抑制された。特にB-2、C-1においては高い発病抑制効果があり、病原菌単独処理区の半分以下に抑制していた。商品価値調査においてはV. lecanii処理区で商品価値のあるダイコンの数が優った。以上の結果から、病原菌単独処理と有意な差が認められなかったものもあるが、V. lecaniiはダイコンバーティシリウム黒点病を抑制するのに有効である傾向が確認された。よって、V. lecaniiはダイコンバーティシリウム黒点病に対して生物防除資材として有望であることが示唆された。 Seven strains of Verticillium lecanii were tested for their capacity to control Verticillium black-spot of Japanese radish...
Palavras-chave: ダイコンバーティシリウム黒点病; Black spot of Japanese radish; Verticillium dahliae; Verticillium lecanii.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/96
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貯留中の乳牛ふん尿スラリーから発生する環境負荷ガスを抑制する撥水加工肥料資材の開発と評価 OAK
坂本, 直久; 谷, 昌幸; 小池, 正徳; 倉持, 勝久; 岸本, 正; 梅津, 一孝; Sakamoto, Naohisa; Tani, Masayuki; Koike, Masanori; Kishimoto, Tadashi; Umetsu, Kazutaka; Kuramochi, Katsuhisa.
本研究では,貯留中の乳牛ふん尿スラリーから発生する環境負荷ガスを低減するため,化学肥料を撥水加工した革新的なカバー資材を開発し,その抑制効果の評価とメカニズムについて検証した。スラリーは,メタン発酵前と発酵後の2種類を用いた。資材は,スラリー上に浮かべることを可能とし,また農地還元する上で不足する植物養分を補給するために,微粉砕した化学肥料に疎水性シリカを混合することにより調製した。実験室規模で13日間のガス発生量を測定した。アンモニアやメタンの積算発生量はメタン発酵後のスラリーの方が発酵前のそれよりも高かった。本資材を供試することにより,スラリーから発生するガスの吸着またはスラリーの発酵挙動が変化し,アンモニアやメタンの発生量が著しく減少した。さらに,資材の一部が溶解してスラリーの養分量が増加するので,本技術は農作業の効率化が図られるとともに,畜産廃棄物の有効利用に役立つと考えられる。 The objectives of this study were to verify the effectiveness in reducing polluting gas emissions of novel methods for covering dairy slurry by using various types of hydrophobic fertilizers and to evaluate the mechanisms for reducing gas emissions from this slurry before and after methane fermentation. The hydrophobic fertilizers were prepared by mixing...
Palavras-chave: 畜産廃棄物; スラリー貯留槽; アンモニア; 温室効果ガス; 環境汚染; Animal waste; Slurry store; Ammonia; Greenhouse gas; Environmental pollution.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2832
Registros recuperados: 15
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