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Registros recuperados: 5
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Altered indirect hemagglutination method for easy serotyping of Haemophilus parasuis Arq. Bras. Med. Vet. Zootec.
Lorenson,M.S.; Miani,M.; Guizzo,J.A.; Barasoul,B.; Martínez-Martínez,S.; Rodríguez-Ferri,E.F.; Gutiérrez-Martín,C.B.; Kreutz,L.C.; Frandoloso,R..
ABSTRACT Glässer's disease is an emergent bacterial disease that affects swine husbandries worldwide causing important economic losses. The aetiological agent, Haemophilus parasuis, is currently divided in fifteen serovars but an increasing number of non-typeable serovars have been reported. Indirect hemagglutination (IHA) is indicated as a serotyping method for H. parasuis. In the present study, we describe an additional step that aims to work around a possible obstacle in the original protocol that may compromise the outcome of this assay. We observed that the choice of anticoagulant for blood collection influences and/or impairs spontaneous adsorption of H. parasuis antigens on sheep red blood cells (SRBCs). However, regardless of the anticoagulant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Haemophilus parasuis; Diagnosis; Tannic acid; Serotyping; Indirect hemagglutination.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000100015
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Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants BJMBR
Pavan,T.R.; Di Domenico,J.; Kirsten,K.S.; Nied,C.O.; Frandoloso,R.; Kreutz,L.C..
Adjuvants are essential to boost the immune response to inoculated antigen and play a central role in vaccine development. In this study, we investigated the efficacy of several adjuvants in the production of anti-bovine serum albumin (BSA) antibodies in silver catfish. Two hundred and seventy juvenile silver catfish (60–80 g) of both sexes were intraperitoneally vaccinated with BSA (200 µg/fish) alone or mixed to the following adjuvants: Freund’s complete adjuvant (FCA), Freund’s incomplete adjuvant (FIA), aluminum hydroxide (AlOH), Montanide, four types of cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) and three concentrations of β-glucan, and the immune enhancing property was evaluated by measuring anti-BSA antibodies in blood samples at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vaccination; Fish; Immune response; CpGs ODN; Β-glucan.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800606
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Inactivated Parapoxvirus ovis induces a transient increase in the expression of proinflammatory, Th1-related, and autoregulatory cytokines in mice BJMBR
Anziliero,D.; Weiblen,R.; Kreutz,L.C.; Spilki,F.; Flores,E.F..
The immunostimulatory properties of inactivated Parapoxvirus ovis (iPPVO) have long been investigated in different animal species and experimental settings. In this study, we investigated the effects of iPPVO on cytokine expression in mice after intraperitoneal inoculation. Spleen and sera collected from iPPVO-treated mice at intervals after inoculation were submitted to cytokine mRNA determination by real-time PCR (qPCR), serum protein concentration by ELISA, and interferon (IFN)-α/β activity by bioassay. The spleen of iPPVO-treated animals showed a significant increase in mRNA expression of all cytokines assayed, with different kinetics and magnitude. Proinflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-alpha (TNF-α), and IL-8 mRNA peaked...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytokine; MRNA; ORF virus; Immunostimulant; Innate immune response.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200110
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Increased immunoglobulin production in silver catfish (Rhamdia quelen) exposed to agrichemicals BJMBR
Kreutz,L.C.; Pavan,T.R.; Alves,A.G.; Correia,A.G.; Barriquel,B.; dos Santos,E.D.; Barcellos,L.J.G..
Fish vaccination has been increasingly exploited as a tool to control pathogen infection. The production of immunoglobulin following vaccination might be affected by several factors such as management procedures, water temperature, and the presence of xenobiotics. In the present study, we aimed to investigate the kinetics of immunoglobulin production in silver catfish (Rhamdia quelen) inoculated with inactivated Aeromonas hydrophila and kept at two different water temperatures (17.4±0.4° or 21.3±0.3°C). The effect of a second antigen inoculation and exposure of fish to sublethal concentrations of the herbicides atrazine and glyphosate at 10% of the lethal concentration (LC50-96h) on specific serum antibodies were also investigated. Antibodies to A....
Palavras-chave: Fish; Humoral response; Antibody production; Herbicides; Adjuvant.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600499
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Production and characterization of monoclonal antibodies to Brazilian isolates of bovine viral diarrhea virus BJMBR
Kreutz,L.C.; Donis,R.; Gil,L.H.V.; Lima,M.; Hoffman,A.N.; Garcez,D.C.; Flores,E.F.; Weiblen,R..
Three Brazilian isolates of bovine viral diarrhea virus (BVDV), antigenically distinct from the standard North American isolates, were selected to immunize BALB/c mice in order to obtain hybridoma cells secreting anti-BVDV monoclonal antibodies (mAbs). Two hybridoma clones secreting mAbs, reacting specifically with BVDV-infected cells (mAbs 3.1C4 and 6.F11), were selected after five fusions and screening of 1001 hypoxanthine-aminopterin-thymidine-resistant clones. These mAbs reacted in an indirect fluorescent antibody (IFA) assay with all 39 South and North American BVDV field isolates and reference strains available in our laboratory, yet failed to recognize other pestiviruses, namely the hog cholera virus. The mAbs reacted at dilutions up to 1:25,600...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine viral diarrhea virus; Monoclonal antibodies; Antigenic diversity; Diagnostic reagents.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200010
Registros recuperados: 5
Primeira ... 1 ... Última
 

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