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A new artificial diet for the early weaning of seabass (<i>Dicentrarchus labrax</i>) larvae OMA
Devresse, B.; Candreva, P.; Léger, P.; Sorgeloos, P..
The weaning of marine fish larvae is a critical step in larval rearing. The successful transition from live food to an artificial feed depends among others on the quality of the dry feed used (e.g. freshness of ingredients, digestibility, quality of the formulation) and the larvae themselves (e.g. age, behaviour towards artificial diets, development of the digestive tract, efficiency of certain metabolic pathways). The present study shows the results obtained with a new artificial diet formulated for the weaning phase of marine fish larvae (<i>Dicentrarchus labrax</i>) in commercial hatcheries.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artificial feeding; Feed composition; Fish culture; Fish larvae; Hatcheries; Dicentrarchus labrax [Bass].
Ano: 1991 URL: http://www.vliz.be/imisdocs/publications/262322.pdf
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A new test system for the evaluation of toxic levels of liposoluble products in the aquatic food chain using <i>Artemia</i> and <i>Mysidopsis bahia</i> as experimental animals OMA
Van Sprang, P.; Léger, P.; Sorgeloos, P..
A new test system is described for the evaluation of toxic levels of liposoluble products in the aquatic food chain using <i>Artemia</i> and <i>Mysidopsis bahia</i> (M.) as experimental animals. For this, freshly released juveniles (<24h old) of <i>M. bahia</i> are transferred to the experimental bioassay test system, cultured under standard conditions and fed 24h old purposely contaminated <i>Artemia</i> nauplii. After 11 days, data on survival, reproductive characteristics, individual length and dry weight are recorded and treated statistically.
Tipo: Info:eu-repo/semantics/article
Ano: 1991 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2910
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Advances in the enrichment of rotifers and <i>Artemia</i> as food sources in marine larviculture OMA
Léger, P.; Grymonpré, D.; Van Ballaer, E.; Sorgeloos, P..
A summary is given of experiments conducted regarding the enrichment of rotifers and <i>Artemia</i> for use as food sources marine larviculture, with particular reference to levels of omega 3-HUFA.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brine shrimp culture; Crustacean culture; Fatty acids; Food organisms; Nutritive value; Artemia; Rotifera.
Ano: 1989 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2880
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An intensive approach to Atlantic halibut fry production OMA
Holmefjord, I.; Gulbrandsen, J.; Lein, I.; Refstie, T.; Léger, P.; Harboe, T.; Huse, I.; Sorgeloos, P.; Bolla, S.; Olsen, Y.; Reitan, K.I.; Vadstein, O.; Øie, G.; Danielsberg, A..
The larval stage is regarded to be the main bottleneck of halibut production. Halibut eggs were obtained from captive broodstock both by stripping and by natural spawning. Artificial photoperiods were used to increase the total spawning season. Yolk sac larvae are presently produced either in small stagnant units or in large flow through systems. A major consideration is to avoid stress of the larvae, caused by mechanical disturbances of the larvae and by high bacterial load or high ammonia levels in the water.The experimentation showed that halibut larvae began to ingest algae earlier than rotifers <i>Brachionus</i> sp. Supplementation of algae to first feeding tanks resulted in enhanced survival and growth rate of the larvae. Both rotifers...
Tipo: Info:eu-repo/semantics/article
Ano: 1993 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2709
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Automation in stock-culture maintenance and juvenile separation of the mysid <i>Mysidopsis bahia</i> (Molenonck) OMA
Léger, P.; Sorgeloos, P..
A batch system with subgravel filter for stock-culture maintenance of <i>Mysidopsis bahia</i> in artificial sea water is described. The feeding of mysids on <i>Artemia</i> is optimized through the cyclic pumping of brine shrimp instar I nauplii from a refrigerator to the stock-cultures. An easily constructed incubator-separator apparatus is described for the standardized harvesting of mysid juveniles to be used as test organisms.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquaculture; Automation; Crustacean culture; Mysidopsis bahia.
Ano: 1982 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3419
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Bioinformatic analysis of ESTs collected by Sanger and pyrosequencing methods for a keystone forest tree species : oak Inra
Ueno, S.; Le Provost, G.; Léger, V.; Klopp, C.; Noirot, C.; Frigerio, J.M.; Salin, F.; Salse, J.; Abrouk, M.; Murat, F.; Brendel, O.; Derory, J.; Abadie, P.; Léger, P.; Cabane, C.; Barré, A.; De Daruvar, A.; Couloux, A.; Wincker, P.; Reviron, M.-P.; Kremer, A.; Plomion, C..
Background: The Fagaceae family comprises about 1,000 woody species worldwide. About half belong to the Quercus family. These oaks are often a source of raw material for biomass wood and fiber. Pedunculate and sessile oaks, are among the most important deciduous forest tree species in Europe. Despite their ecological and economical importance, very few genomic resources have yet been generated for these species. Here, we describe the development of an EST catalogue that will support ecosystem genomics studies, where geneticists, ecophysiologists, molecular biologists and ecologists join their efforts for understanding, monitoring and predicting functional genetic diversity.Results: We generated 145,827 sequence reads from 20 cDNA libraries using the Sanger...
Tipo: Journal Article Palavras-chave: POLYMORPHISME NUCLÉOTIDIQUE SIMPLE; RESSOURCE GÉNOMIQUE; EXTRACTION D'ARN; CLONAGE D'ADN; OUTIL D'ANALYSE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011fe822ff&uri=/notices/prodinra1/2011/03/
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Characterization of brine shrimp <i>Artemia</i> from Cam Ranh Bay in Central Vietnam OMA
Quynh, V.D.; Lavens, P.; Léger, P.; Tackaert, W.; Sorgeloos, P..
<i>Artemia</i> cysts collected from inoculation experiments in Cam Ranh salterns are evaluated for their potential use in aquaculture. Cyst biometrics, hatching quality, naupliar fatty acid profile and naupliar growth were measured and compared to reference <i>Artemia</i> strains. Cyst characteristics reveal that the parthenogenetic strain (PR China) used in inoculations, was eliminated from the environment and that the remaining brine shrimp are likely to be composed of Macau and Great Salt Lake <i>Artemia</i> strains, and of their cross-breds. Differences in cyst diapause deactivation characteristics between Macau and Great Salt Lake <i>Artemia</i> may have resulted in the disappearance of Macau...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cysts Inoculation Inoculation Quality Salt lakes Artemia ISEW; Viet Nam; Cam Ranh Bay.
Ano: 1988 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3244
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Characterization of <i>Artemia</i> from different localities in Tunisia with regard to their use in local aquaculture OMA
Van Ballaer, E.; Versichele, D.; Vanhaecke, P.; Léger, P.; Ben Abdelkader, N.; Turki, S.; Sorgeloos, P..
<i>Artemia</i> cysts have been collected from different saltworks and natural salt lakes in Tunisia. The cyst material was processed and used for the following characterization analyses: cyst and naupliar biometrics, cyst hatching characteristics, fatty acid pattern of the nauplii, nutritional value of the nauplii for mysid shrimp, naupliar growth rate and temperature resistance, mode of reproduction, and characterization of sibling species. The cross-breeding tests and cyst biometrics reveal that only <i>Artemia tunisiana</i> occurs in Tunisia. Although quality improvements may be expected through improved harvesting, Tunisian <i>Artemia</i> have acceptable hatching characteristics and are a good food for mysid shrimp....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artemia; Tunisia.
Ano: 1987 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3207
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Contrasting patterns of selection at Pinus pisaster Ait. drought stress candidate genes as revealed by genetic differentiation analyses Inra
Eveno, E.; Collada, C.; Guevara, M.A.; Léger, V.; Soto, A.; Diaz, L.; Léger, P.; Gonzalez-Martinez, S.C.; Cervera, M.T.; Plomion, C.; Garnier-Géré, P..
Tipo: Journal Article Palavras-chave: EVOLUTION; GENE CANDIDAT.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008deda62&uri=/notices/prodinra1/2008/05/
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Description of a standard bioassay with the marine crustacean <i>Mysidopsis bahia</i> (M.) for the evaluation of the nutritional effectiveness of <i>Artemia</i> nauplii and metanauplii OMA
Léger, P.; Johns, D.; Sorgeloos, P..
A standardized bioassay is described for the nutritional evaluation of <i>Artemia</i> nauplii and metanauplii using juvenile <i>Mysidopsis bahia</i>. This test is particularly suited to evaluate the dietary value of different strains and batches of <i>Artemia</i> . One day starved, fed or enriched <i>Artemia</i> metanauplii can also be tested.For this test, a homogenous population of freshly-released juveniles (less or equal to 24h-old) were produced in a mysid generator-separator. The test animals were transferred into an experimental apparatus, cultured under standard conditions and offered <i><i>Artemia</i> ad libitum</i>. After 12 days, data on survival, length, individual dry...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artemia; Mysidopsis bahia.
Ano: 1987 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3181
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International study on <i>Artemia</i> : 24. Cold storage of live <i>Artemia</i> nauplii from various geographical sources: potentials and limits in aquaculture OMA
Léger, P.; Vanhaecke, P.; Sorgeloos, P..
Freshly-hatched <i>Artemia</i> nauplii from various geographical sources survived storage in a refrigerator (2-4°C) at densities of 2000 per ml and above. Except for <i>Artemia</i> from Chaplin Lake and Buenos Aires, naupliar viability was very high even after 48 h storage, and did not decrease significantly after a 24 h post-storage transfer to 25°C. Neither the naupliar dry weight nor biochemical composition changed significantly during refrigeration for most strains tested. Comparative culture-tests with stored and freshly-hatched nauplii as food for juvenile marine mysids <i>Mysidopsis bahia</i> M. and larval carp <i>Cyprinus carpio</i> L. revealed similar production performances.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquaculture techniques; Cold storage; Nauplii; Artemia.
Ano: 1983 URL: http://www.vliz.be/imisdocs/publications/261894.pdf
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International study on <i>Artemia</i> : 25. Factors determining the nutritional effectiveness of <i>Artemia</i> : the relative impact of chlorinated hydrocarbons and essential fatty acids in San Francisco Bay and San Pablo Bay <i>Artemia</i> OMA
Léger, P.; Sorgeloos, P.; Millamena, O.M.; Simpson, K.L..
Different <i>Artemia</i> cyst samples harvested from the San Francisco and the San Pablo Bay regions (California, USA), and suspect because of their poor nutritional performance in fish and crustacean farming, have been analyzed for their chlorinated hydrocarbon and fatty acid content. These results have been correlated with survival, growth, and biomass production of larvae of the marine crustacean <i>Mysidopsis bahia</i> Molenock fed those different <i>Artemia</i> in a standard culture test. Differences in chlorinated hydrocarbon content do not correlate with differences in mysid culture performance. Fatty acid profiles reveal three groups of <i>Artemia</i> batches with high, intermediate, and low levels of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorinated hydrocarbons Fatty acids Nutritional requirements Artemia INE; USA; California; San Francisco Bay INE; USA; California; San Pablo Bay.
Ano: 1985 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3965
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International study on <i>Artemia</i> : 29. Nutritional evaluation of <i>Artemia</i> nauplii from different geographical origin for the marine crustacean <i>Mysidopsis bahia</i> OMA
Léger, P.; Sorgeloos, P..
Freshly hatched nauplii from reference <i>Artemia</i> and from 3 other <i>Artemia</i> strains were tested for their effects on survival, growth and reproductive characteristics of the crustacean <i>Mysidopsis bahia</i> (M.). The reference, French (Lavalduc) and Chinese (Tientsin) strains produced significantly better results for all criteria tested than the Canadian strain (Chaplin Lake). Results are discussed together with those reported for other predators.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nauplii; Artemia; Mysidopsis bahia.
Ano: 1984 URL: http://www.vliz.be/imisdocs/publications/261918.pdf
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International study on <i>Artemia</i> : 33. Promising results in larval rearing of <i>Penaeus stylirostris</i> using a prepared diet as algal substitute and for <i>Artemia</i> enrichment OMA
Léger, P.; Bieber, G.F.; Sorgeloos, P..
The importance of highly unsaturated fatty acids (HUFA) such as 20:5omega3 and 22:6omega3 has been investigated for the larvae of the blue shrimp <i>Penaeus stylirostris</i>. HUFA-rich or HUFA-enriched <i>Artemia</i> nauplii yielded higher production results in the hatchery than HUFA-poor <i>Artemia</i> especially when yeast was used as a substitute for algae during the early larval stages. Best results were obtained when a HUFA-rich formulated diet was used as a supplement to the yeast and as an enrichment for the <i>Artemia</i> nauplii. Differences in production results could be attributed to the HUFA content in living and non-living larval diets.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diets; Food organisms; Rearing; Shrimp culture; Artemia; Penaeus stylirostris (Stimpson).
Ano: 1985 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3234
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International study on <i>Artemia</i> : 35. Techniques to manipulate the fatty acid profile in <i>Artemia</i> nauplii, and the effect on its nutritional effectiveness for the marine crustacean <i>Mysidopsis bahia</i> (M.) OMA
Léger, P.; Naessens-Foucquart, E.; Sorgeloos, P..
The importance of essential fatty acids for the early larval stages of cultured marine fish and crustacean larvae is well documented. In this regard, the variability in essential fatty acid content of their live prey <i>Artemia</i> is critical.This paper describes different techniques for improving the fatty acid profile in <i>Artemia</i> nauplii using microparticles and emulsions as enrichment diets. These diets can be applied during the incubation of the cysts and/or after the separation of the nauplii. After enrichment, the nauplii not only contain high levels of the essential fatty acid 20:5 omega 3 (up to 13.5% or 35.2 mg/g) but also considerable levels of 22:6 omega 3 (up to 7% or 18.1 mg/g), another essential fatty acid which...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artemia; Mysidopsis bahia.
Ano: 1987 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3184
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Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (<i>Artemia</i> spp.) used for prophylactic chemotherapy of fish OMA
Nelis, H.; Léger, P.; Sorgeloos, P.; De Leenheer, A.P..
The brine shrimp <i>Artemia</i> , an excellent live food source in aquaculture, has been studied as a carrier to deliver selected chemotherapeutic agents to fish for prophylactic treatment of infectious diseases. To monitor the efficiency of incorporation of trimethoprim and sulfamethoxazole in <i><i>Artemia</i> franciscana</i>, a sensitive and specific analytical method was developed. It is based on homogenization of <i>Artemia</i> nauplii in methanol, extraction of lipids with hexane, solid-phase cleanup on C<sub>18</sub> cartridges, and reversed-phase liquid chromatography with detection at 210 nm. The method is sensitive (detection limit, on the order of 3 µg/g with a sample quantity of 30 mg...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquaculture techniques; Fish culture; Infectious diseases; Medicine; Artemia.
Ano: 1991 URL: http://www.vliz.be/imisdocs/publications/262329.pdf
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Live-food mediated drug delivery as a tool for disease treatment in larviculture: the enrichment of therapeutics in rotifers and <i>Artemia</i> nauplii OMA
Verpraet, R.; Chair, M.; Léger, P.; Nelis, H.; Sorgeloos, P.; De Leenheer, A.P..
The live food enrichment technique using emulsions for nutritional deficiencies in rotifers and <i>Artemia</i> was further investigated as a tool for transferring therapeutics through the food chain. <i>Artemia</i> nauplii and the rotifer <i>Brachionus plicatilis</i> were enriched with a self-emulsifying concentrate in which the therapeutic mixture trimetoprim: sulfamethoxazole was incorporated. An analytical method and protocol for the quantification of trimethoprim and sulfamethoxazole in a heterogeneous biological matrix, such as <i>Artemia</i> , was developed using High Performance Liquid Chromatography. When applying a 24h enrichment period, the accumulation of both drugs in <i>Artemia</i>...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquaculture systems; Disease control; Drugs; Food chains; Therapy; Artemia.
Ano: 1992 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2894
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Manipulated yeast diets as a partial algal substitute for rearing bivalve molluscs: laboratory trials with <i>Tapes semidecussata</i> OMA
Coutteau, P.; Lavens, P.; Léger, P.; Sorgeloos, P..
The development of a cost-effective algal substitute would greatly reduce operating costs of bivalve hatcheries and has therefore been the subject of many studies. Previous work has resulted in the development of a yeast diet with improved digestibility and nutritional composition. Preliminary trials showed that this diet can substitute up to 80% of the algal diet in the seed culture of the Manila clam <i>Tapes semidecussata</i>. In this communication we propose a culture test diet which will be used to study further dietary improvements of the yeast product.Juveniles of the Manila clam (1 to 5 mg unit weight) were batch cultured in a 51 recirculating system. Optimal daily ration for the algal control diet <i>Chaetoceros...
Tipo: Info:eu-repo/semantics/article
Ano: 1990 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2812
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(n-3) HUFA composition of freshly spawned eggs from European seabass (<i>Dicentrarchus labrax</i>), seabream (<i>Sparus aurata</i>) and red seabream (<i>Pagrus major</i>) collected in different hatcheries OMA
Komis, A.; Léger, P.; Sorgeloos, P..
The biochemical composition of fish eggs is often used as an indicator for assessing the nutritional requirements of aquaculture species during their larval stages. Fatty acids of the (n-3) HUFA type have proven to play a critical role in providing good growth, survival and stress resistance in marine fish larvae. Hence, analyzing these fatty acids in fish eggs may provide interesting data on the requirements for those essential nutrients. By comparing the analyses of wild versus broodstock fish eggs differences may appear, possibly explaining differences in egg quality. Intra-spawning-season differences may further appear by following fatty acid profiles in the course of time. Finally, the influence of broodstock nutritionon egg composition may be another...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Comparative studies; Fatty acids; Fish culture; Fish eggs; Hatcheries; Induced breeding; Dicentrarchus labrax [Bass]; Pagrus major; Sparus aurata [Gilthead].
Ano: 1991 URL: http://www.vliz.be/imisdocs/publications/262325.pdf
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New developments in the mass culture and nutritional enrichment of the rotifer <i>Brachionus plicatilis</i> using artificial diets OMA
Komis, A.; Naessens, E.; Van Ballaer, E.; Van Sprang, P.; Léger, P.; Sorgeloos, P..
A research program was carried out to improve and facilitate rotifer (<i>Brachionus plicatilis</i>) culturing as well as to assure their optimal nutritional quality as a prey, concentrating on the development of a dry and complete rotifer diet that does not require the use of algae and does result in a HUFA rich <i>Brachionus</i>. The present poster provides results dealing with culture aspects and rotifer nutritional quality. Different diet compositions based on single cell proteins enriched with various nutritional additives were evaluated. Results obtained so far demonstrate that a selected formula of a combined culture and enrichment diet, that does not involve the use of microalgae at all, can be successfully used for growing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artificial feeding; Diets; Food organisms; Mass culture; Nutritive value; Brachionus plicatilis.
Ano: 1989 URL: http://www.vliz.be/imisdocs/publications/262326.pdf
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