|
|
|
|
| |
|
| |
|
|
OLIVEIRA, E. R.; FAYER, L.; ZANETTE, R. S. S.; LADEIRA, L. O.; OLIVEIRA, L. F. C. de; MARANDUBA, C. M. C.; BRANDAO, H. de M.; MUNK, M.. |
Carboxylated Multi-wall Carbon Nanotube (MWCNT-COOH) presents unique properties due to nanoscale dimensions and permits a broad range of applications in different fields, such as bone tissue engineering and regenerative medicine. However, the cytocompatibility of MWCNT-COOH with human stem cells is poorly understood. Thus, studies elucidating how MWCNT-COOH affects human stem cell viability are essential to a safer application of nanotechnologies. Using stem cells from the human exfoliated deciduous teeth model, we have evaluated the effects of MWCNT-COOH on cell viability, oxidative cell stress, and DNA integrity. Results demonstrated that despite the decreased metabolism of mitochondria, MWCNT-COOH had no toxicity against stem cells. Cells maintained... |
Tipo: Artigo de periódico |
Palavras-chave: Nanotecnologia; Estudo in vitro; Engenharia de tecido; Células-tronco; Carbono. |
Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135728 |
| |
|
|
PEREIRA, M. M; MOUTON, L.; YÉPRÉMIAN, C.; COUTÉ, A.; LO, J.; MARCONCINI, J. M.; LADEIRA, L. O.; RAPOSO, N. RB; BRANDÃO, H. M.; BRAYNER, R.. |
Tipo: Artigo de periódico |
Palavras-chave: Nanopartícula; Uptake; Nanotoxicologia; Bioindicador; Microalga. |
Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1007487 |
| |
|
|
PEREIRA, M. M; MOUTON, L.; YÉPRÉMIAN, C.; COUTÉ, A.; LO, J.; MARCONCINI, J. M.; LADEIRA, L. O.; RAPOSO, N. R. B.; BRANDAO, H. de M.; BRAYNER, R.. |
Tipo: Separatas |
Palavras-chave: Bioindicador; Nanopartícula; Nanotoxicologia; Uptake.; Microalga.. |
Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1010348 |
| |
|
| |
|
|
MUNK, M.; LADEIRA, L. O.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; RAPOSO, N. R. B.; SERAPIÁO, R. V.; QUINTAO, C. C. R.; SILVA, S. R.; SOARES, J. S.; JORIO, A.; BRANDAO, H. de M.. |
The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher... |
Tipo: Separatas |
Palavras-chave: Gene delivery; Pellucid zone.; In vitro fertilization.. |
Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1064560 |
| |
|
| |
|
|
|