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| SOARES JUNIOR, F. L.; DIAS, A. C. F.; FASANELLA, C. C.; TAKETANI, R. G.; LIMA, A. O. S.; MELO, I. S. de; ANDREOTE, F. D.. |
| The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from amangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria,enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplementedwith 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33... |
| Tipo: Separatas |
Palavras-chave: Cellulose; Endo-1; 4-B-D-glucanase Exo-1; 4-B-D-glucanase. salinity.. |
| Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/981473 |
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| KMIT, M. C. P.; LIMA, A. O. S.; FREITAS, R. C.; ROMAGNOLI, E. M.; ABDALLA, A. L.; MENDES, R.. |
| The rumen harbors complex microbial communities which participate in an efficient process to digest plant biomass. This ecosytem represents an untapped source of hydrolytic enzymes with potential application for second?generation biofuel production from lignocellulosic biomass. The search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to describe the functions and explores the potential for lignocellulolitic enzyme in the sheep rumen microbiome. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two... |
| Tipo: Separatas |
Palavras-chave: Enzima; Rúmen.. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1071445 |
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| KMIT, M. C. P.; LIMA, A. O. S.; ROMAGNOLI, E. M.; ABDALLA, A. L.; MENDES, R.. |
| Abstract: The lignocellulose present in the plant biomass, is a promising source of energy generation. However, the breakdown of plant biomass into simple sugars for bioethanol production is still inefficient and costly due to the recalcitrant nature of plant fiber. Sheep rumen microbiome is specialized in degradation of plant material, but most members of this complex community are non-cultivable in laboratory. Therefore, the search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to obtain plant biomass-degrading genes, selected from the... |
| Tipo: Separatas |
Palavras-chave: Lignocelluloytic enzymes; Secind generation biofuel; Microbial metagenome; Enzima; Biotecnologia; Metagenomics. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036656 |
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