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Registros recuperados: 14 | |
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SILVA, M. C. M. da; SARTO, R. P. del; LUCENA, W. A.; RIGDEN, D. J.; TEIXEIRA, F. R.; BEZERRA, C. de A.; ALBUQUERQUE, E. V. S.; GROSSI DE SA, M. F.. |
Numerous species of insect pests attack cotton plants, out of which the cotton boll weevil (Anthonomus grandis) is the main insect in Brazil and must be controlled to avert large economic losses. Like other insect pests, A. grandis secretes a high level of -amylases in the midgut lumen, which are required for digestion of carbohydrates. Thus, -amylase inhibitors (-AIs) represent a powerful tool to apply in the control of insect pests. Here, we applied DNA shuffling and phage display techniques and obtained a combinatorial library containing 108 ˛-AI variant forms. From this library, variants were selected exhibiting in vitro affinity for cotton boll weevil -amylases. Twenty-six variant sequences were cloned into plant expression vectors and... |
Tipo: Separatas |
Palavras-chave: Alfa-amylase inhibitors; In vitro molecular evolution.; Anthonomus Grandis.; DNA shuffling; Insect control.. |
Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/979826 |
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BEZERRA, C. A.; MACEDO, L. L. P.; AMORIM, T. M. L.; SANTOS, V. O.; FRAGOSO, R. da R.; LUCENA, W. A.; MENEGUIM, A. M.; VALENCIA-JIMENEZ, A.; ENGLER, G.; SILVA, M. C. M. da; SA, M. F. G. de; FREIRE, E. V. S. A.. |
Abstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect... |
Tipo: Separatas |
Palavras-chave: Expressão gênica; Curculionideo; Praga de planta; Amido; Hidrolise; Curculionidae; Gene expression; Insect pests; Starch; Enzymatic hydrolysis. |
Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1003023 |
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BEZERRA, C. A.; MACEDO, L. L. P.; AMORIM, T. M. L.; SANTOS, V. O.; FRAGOSO, R. da R.; LUCENA, W. A.; MENEGUIM, A. M.; VALENCIA-JIMENEZ, A.; ENGLER, G.; SILVA, M. C. M.; ALBUQUERQUE, E. V. S.; GROSSI-DE-SA, M. F.. |
Abstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect... |
Tipo: Separatas |
Palavras-chave: Expressão gênica; Curculionideo; Praga de planta; Amido; Hidrolise; Curculionidae; Gene expression; Insect pests; Starch; Enzymatic hydrolysis. |
Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1006141 |
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RIBEIRO, T. P.; BASSO, M. F.; CARVALHO, M. H. de; MACEDO, L. L. P. de; SILVA, D. M. L. da; LOURENCO-TESSUTTI, I. T.; OLIVEIRA-NETO, O. B. de; CAMPOS-PINTO, E. R. de; LUCENA, W. A.; SILVA, M. C. M. da; TRIPODE, B. M. D.; ABREU-JARDIM, T. P. F.; MIRANDA, J. E.; ALVES-FERREIRA, M.; MORGANTE, C. V.; GROSSI-DE-SA, M. F.. |
Tipo: Artigo de periódico |
Palavras-chave: Stable inheritance; Insect pest; Resistance; Management; Bacillus; Thuringiensis; Entomotoxin. |
Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124665 |
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RIBEIRO, T. P.; BASSO, M. F.; CARVALHO, M. H. de; MACEDO, L. L. P. de; SILVA, D. M. L. da; LOURENCO-TESSUTTI, I. T.; OLIVEIRA-NETO, O. B. de; CAMPOS-PINTO, E. R. de; LUCENA, W. A.; SILVA, M. C. M. da; TRIPODE, B. M. D.; ABREU-JARDIM, T. P. F.; MIRANDA, J. E.; ALVES-FERREIRA, M.; MORGANTE, C. V.; GROSSI-DE-SA, M. F.. |
The cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower... |
Tipo: Artigo de periódico |
Palavras-chave: Praga de inseto; Resistência gestão; Bacillus; Bicudo do algodão; Herança estável; Algodão; Inseto; Praga; Praga de Planta; Insect pests; Resistance management; Cotton. |
Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125667 |
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RIBEIRO, T. P.; ARRAES, F. B. M.; LOURENCO-TESSUTTI, I. T.; SILVA, M. S.; LISEI-DE-SÁ, M. E.; LUCENA, W. A.; MACEDO, L. L. P. de; LIMA, J. N.; AMORIM, R. M. S.; ARTICO, S.; ALVES-FERREIRA, M.; SILVA, M. C. M.; GROSSI-DE-SA, M. F.. |
Tipo: Separatas |
Palavras-chave: Plant resistance; Bt toxin.; Anthonomus Grandis; Gossypium Hirsutum.; Genetic transformation; Insect pests.. |
Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1072905 |
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OLIVEIRA, R. S. de; OLIVEIRA NETO, O. B.; MOURA, H. F. N.; MACEDO, L. L. P. de; ARRAES, F. B. M.; LUCENA, W. A.; LOURENCO TESSUTTI, I. T.; BARBOSA, A. A. de D.; SILVA, M. C. M. da; GROSSI DE SA, M. F.. |
Tipo: Separatas |
Palavras-chave: Genetic cotton transformation; Pollen-tube pathway; Cry1Ia12; Spodoptera frugipe; Anthonomus Grandis; Gossypium Hirsutum. |
Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053366 |
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OLIVEIRA, R. S. de; OLIVEIRA NETO, O. B.; MOURA, H. F. N.; MACEDO, L. L. P. de; ARRAES, F. B M.; LUCENA, W. A.; LOURENCO, I. T.; BARBOSA, A. de D.; SILVA, M. C. M. da; SA, M. F. G. de. |
Gossypium hirsutum (commercial cooton) is one of the most economically important fibers sources and a commodity crop highly affected by insect pests and pathogens. Several transgenic approaches have been developed to improve cotton resistance to insect pests, through the transgenic expression of different factors, including Cry toxins, proteinase inhibitors, and toxic peptides, among others. In the present study, we developed transgenic cotton plants by fertilized floral buds injection (through the pollen-tube pathway technique) using an DNA expression cassette harboring the cry1Ia12 gene, driven by CaMV35S promoter. |
Tipo: Artigo de periódico |
Palavras-chave: Gosspypium hirsutum; Genetic cotton transformation; Polen-tube pathway. |
Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1066050 |
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Registros recuperados: 14 | |
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