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Assessing chronic fish health: An application to a case of an acute exposure to chemically treated crude oil ArchiMer
Mauduit, Florian; Domenici, P.; Farrell, A. P.; Lacroix, C.; Le Floch, S.; Lemaire, P.; Nicolas-kopec, A.; Whittington, M.; Zambonino-infante, Jose-luis; Claireaux, Guy.
Human alteration of marine ecosystems is substantial and growing. Yet, no adequate methodology exists that provides reliable predictions of how environmental degradation will affect these ecosystems at a relevant level of biological organization. The primary objective of this study was to develop a methodology to evaluate a fish’s capacity to face a well-established environmental challenge, an exposure to chemically dispersed oil, and characterize the long-term consequences. Therefore, we applied high-throughput, non-lethal challenge tests to assess hypoxia tolerance, temperature susceptibility and maximal swimming speed as proxies for a fish’s functional integrity. These whole animal challenge tests were implemented before (1 month) and after (1 month)...
Tipo: Text Palavras-chave: Fish; Oil spill; Dispersant; Biomarkers; Physiological integrity; Ecological performance; Temperature; Hypoxia; Swimming; Growth.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00347/45828/45916.pdf
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Pleiotropic role of quorum-sensing autoinducer 2 in Photorhabdus luminescens Inra
Krin, E.; Chakroun, N.; Turlin, E.; Givaudan, A.; Gaboriau, F.; Bonne, I.; Rousselle, J.C.; Frangeul, L.; Lacroix, C.; Hullo, M.F.; Marisa, L.; Danchin, A.; Derzelle, S..
Bacterial virulence is an integrative process that may involve quorum sensing. In this work, we compared by global expression profiling the wild-type entomopathogenic Photorhabdus luminescens subsp. laumondii TT01 to a luxS-deficient mutant unable to synthesize the type 2 quorum-sensing inducer AI-2. AI-2 was shown to regulate more than 300 targets involved in most compartments and metabolic pathways of the cell. AI-2 is located high in the hierarchy, as it controls the expression of several transcriptional regulators. The regulatory effect of AI-2 appeared to be dose dependent. The luxS-deficient strain exhibited decreased biofilm formation and increased type IV/V pilus-dependent twitching motility. AI-2 activated its own synthesis and transport. It also...
Tipo: Journal Article Palavras-chave: BACTERIAL VIRULENCE; OXIDATIVE STRESS; PROTEOME ANALYSIS; OXIDATIVE STRESS.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20085c5590af&uri=/notices/prodinra1/2009/03/
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Use of an immobilized cell bioreactor for the continuous inoculation of milk in fresh cheese manufacturing Inra
Sodini, I.; Boquien, C.Y.; Corrieu, G.; Lacroix, C..
A system was developed to continuously acidify and inoculate skim milk for the production of fresh cheese. Four strains of mesophilic lactic acid bacteria were entrapped separately in κ -carrageenan/locust bean gum gel beads and used in a stirred bioreactor operated at 26°C with a 25% (v/v) gel load. The pH in the reactor was controlled at 6.0 by adding fresh milk using proportional integrated derived regulation. The bioreactor was operated during 8-h daily cycles for up to 7 weeks with different milks (heat treatment, dry matter content) and differing starting procedures. The heat treatment of the milk was an important factor for process performance: a dilution rate increase of 57% and an inoculation level decrease of 63% were observed with sterilized UHT...
Tipo: Journal Article Palavras-chave: FERMENTATION LACTIQUE; PH; TENEUR EN EAU; CONTROLE CONTINU LACTIC ACID BACTERIA; FRESH CHEESE; MILK; IMMOBILIZATION; CONTINUOUS INOCULATION; Κ-CARRAGEENAN GEL BEADS.
Ano: 1997 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0100006104086253&uri=/notices/prodinra1/2010/11/
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