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Organogenesis from in vitro-derived leaf and internode explants of Hoya wightii ssp. palniensis -a vulnerable species of Western Ghats 52
Lakshmi,Subbaiah Revathi; Benjamin,Jambarapu Herald Franklin; Kumar,Tirupathi Senthil; Murthy,Garimella Venkata Suryanarayana; Rao,Mandali Venkateswara.
An efficient system was developed for indirect plant regeneration from in vitro-derived leaf and internode explants of Hoya wightii ssp. palniensis. Maximum percentage of the organogenic callus was obtained on MS medium supplemented with NAA (1.0 mg/l) and 2,4-D (2.0 mg/l). The best shoot bud induction was observed on MS medium with BA (1.0 mg/l) +IBA (0.5 mg/l). The coconut water (15%) was better, resulting in a differentiation of the shoot initials in to well-developed shoots. The elongated shoots (› 3cm long) were rooted on a full strength MS basal medium, supplemented with 0.2 mg/l of IBA. Finally, the rooted plants were transferred to the soil with 80% success rate. This protocol was utilized for the in vitro propagation of this endangered plant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro explants; Organogenesis; Hoya wightii ssp. Palniensis; Vulnerable plant; Growth regulators.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300010
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