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| Registros recuperados: 52 | |
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| Long, Marc; Tallec, Kevin; Soudant, Philippe; Lambert, Christophe; Le Grand, Fabienne; Sarthou, Geraldine; Jolley, Dianne; Hegaret, Helene. |
| Harmful microalgal blooms are a threat to aquatic organisms, ecosystems and human health. Toxic dinoflagellates of the genus Alexandrium are known to produce paralytic shellfish toxins and to release bioactive extracellular compounds (BECs) with potent cytotoxic, hemolytic, ichtyotoxic and allelopathic activity. Negative allelochemical interactions refer to the chemicals that are released by the genus Alexandrium and that induce adverse effects on the physiology of co-occurring protists and predators. Releasing BECs gives the donor a competitive advantage that may help to form dense toxic blooms of phytoplankton. However BECs released by Alexandrium minutum are uncharacterized and it is impossible to quantify them using classical chemical methods.... |
| Tipo: Text |
Palavras-chave: Harmful algal bloom; Alexandrium; Allelopathy; Cytotoxicity; PAM fluorometry; Bioassay. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00453/56424/58116.pdf |
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| Long, Marc; Tallec, Kevin; Soudant, Philippe; Le Grand, Fabienne; Donval, Anne; Lambert, Christophe; Sarthou, Geraldine; Jolley, Dianne F.; Hegaret, Helene. |
| Allelochemical interactions are likely to be a contributing factor explaining the success of large blooms of the harmful marine dinoflagellate Alexandrium, however, the physiological mechanisms of allelochemical interactions remain poorly described. Here we investigated the sub-lethal effects (on an hourly scale) of a filtrate containing allelochemicals from Alexandrium minutum on the physiology of the common diatom Chaetoceros muelleri. The filtrate induced deleterious effects to the diatom physiology within only 30 min of exposure. Esterase activity and photosynthesis were drastically inhibited, with up to 34% of the population being metabolically inactive and up to 30% reduction in photosystem II quantum yield when exposed to the filtrate. In addition,... |
| Tipo: Text |
Palavras-chave: Allelopathy; Alexandrium; Metabolism; Photoinhibition; Lipids; Pigments. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00460/57133/59748.pdf |
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| Roussel, Sabine; Bisch, T; Lachambre, S; Boudry, Pierre; Gervois, Jl; Lambert, Christophe; Huchette, S; Day, R. |
| Domestication of Haliotis tuberculata has only recently begun. During the process, we expect that behavioural and physiological traits may evolve to become more adapted to their captive environment. These modifications may result from intentional selection of production traits or unconscious and unintentional selection due to conditions experienced in the farm environment. To study this process at the earliest stage, the progeny of 3 different broodstocks obtained from wild parents, selected farmed abalone and randomly sampled farmed abalone, were studied. After rearing for 16 mo in separate tanks, offspring from the 3 progenies were placed together in sea cages at the same density. After 3 yr, behavioural traits were studied, and the immune status after a... |
| Tipo: Text |
Palavras-chave: Predation; Hiding behaviour; Haliotis tuberculata; Domestication; Selection; Captive; Abalone. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00487/59841/62989.pdf |
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| Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Guilpain, Patrice; Geairon, Philippe; Razet, Daniel; Seugnet, Jean-luc; Robert, Stephane; Ropert, Michel; Simonne, Charlotte; Hugonnet, Virginie; Moal, Jeanne; Pouvreau, Stephane; Huvet, Arnaud; Samain, Jean-francois; Lambert, Christophe; Soudant, Philippe; Frouel, Stephane; Choquet, Gwenaelle; Delaporte, Maryse; Paillard, Christine. |
| Four laboratories are involved in this multidisciplinary study: the Conchological Laboratory of Normandie (LCN), the Invertebrates Physiology Laboratory (LPI), the Université Bretagne Occidentale (LEMAR IHP) and the Conchological Laboratory of Poitou-Charentes (LCPC). The part of the study which is presented here (thematics "Coastal ecology") was produced in collaboration with the LCN. The data on the Bay of Veys was collected by Michel Ropert. This study aims at observing the "sites" effect on mortality and on the general biology of the animal, as well as measuring some environmental elements (e.g. water temperature, etc.). The geographical sites are Gefosse in the Bay of Veys (East Cotentin) and the Ronce bank in the south of the Marennes Oléron Bay. The... |
| Tipo: Text |
Palavras-chave: Immunologie; Physiologie; Paramètres environnementaux; Croissance; Ecologie côtière; Baies des Veys Cotentin; Ronce Charente Maritime; Comparaison sites; Reproduction; Mortalités; Crassostrea gigas; Huîtres. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/acte-3405.pdf |
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| Gay, Melanie; Waechter, Magali; Lambert, Christophe; Escoubas, Jean-michel; Cochennec, Nathalie; Nicolas, Jean-louis; Berthe, Franck; Le Roux, Frédérique. |
| Nowadays, cupped oyster, Crassostrea gigas, production is the main shellfish production in France. Spats cultivated on most farms are usually collected in the wild, but hatchery spats are becoming more numerous, and represent around 10% of today's cultivated spats. During the summer period, when the temperatures are higher, high mortality rates have been regularly observed in cupped in oyster C. gigas (60 to 100%) spats since 1991, both in hatcheries-nurseries and in their natural habitat. Over the last five years, these episodes have become a big concern for all the professionals. |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Pathogène; Vibrio splendidus; Bactériologie. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3277.pdf |
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| Haberkorn, Hansy; Lambert, Christophe; Le Goic, Nelly; Quere, Claudie; Bruneau, A; Riso, Ricardo; Auffret, Michel; Soudant, Philippe. |
| Effects of simultaneous exposure of Pacific oyster, Crassostrea gigas, to both a harmful dinoflagellate that produces Paralytic Shellfish Toxins (PST), Alexandrium minutum, and cadmium (Cd) and copper (Cu), were assessed. Oysters were exposed to a mix of Cd-Cu with two different diets (i.e. A. minutum or Tisochrysis lutea) and compared to control oysters fed A. minutum or T. lutea, respectively, without metal addition. Metals and PST accumulations, digestive gland lipid composition, and cellular and biochemical hemolymph variables were measured after 4 days of exposure. Oysters exposed to Cd-Cu accumulated about thirty-six times less PSTs than oysters exposed to A. minutum alone. Exposure to Cd-Cu induced significant changes in neutral lipids (increase in... |
| Tipo: Text |
Palavras-chave: Harmful algae; Metals; Oysters; Toxin accumulation; Physiological effects. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00169/28071/26286.pdf |
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| Labreuche, Yannick; Lambert, Christophe; Soudant, Philippe; Boulo, Viviane; Huvet, Arnaud; Nicolas, Jean-louis. |
| The strategies used by bacterial pathogens to circumvent host defense mechanisms remain largely undefined in bivalve molluscs. In this study, we investigated experimentally the interactions between the Pacific oyster (Crassostrea gigas) immune system and Vibrio aestuarianus strain 01/32, a pathogenic bacterium originally isolated from moribund oysters. First, an antibiotic-resistant V. aestuarianus strain was used to demonstrate that only a limited number of bacterial cells was detected in the host circulatory system, suggesting that the bacteria may localize in some organs. Second, we examined the host defense responses to V. aestuarianus at the cellular and molecular levels, using flow-cytometry and real-time PCR techniques. We showed that hemocyte... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Oyster; Pathogenesis; Vibrio aestuarianus; Bivalve immunity. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2182.pdf |
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| Gonzalez-fernandez, Carmen; Tallec, Kevin; Le Goic, Nelly; Lambert, Christophe; Soudant, Philippe; Huvet, Arnaud; Suquet, Marc; Berchel, Mathieu; Paul-pont, Ika. |
| While the detection and quantification of nano-sized plastic in the environment remains a challenge, the growing number of polymer applications mean that we can expect an increase in the release of nanoplastics into the environment by indirect outputs. Today, very little is known about the impact of nano-sized plastics on marine organisms. Thus, the objective of this study was to investigate the toxicity of polystyrene nanoplastics (NPs) on oyster (Crassostrea gigas) gametes. Spermatozoa and oocytes were exposed to four NPs concentrations ranging from 0.1 to 100 mg L−1 for 1, 3 and 5 h. NPs coated with carboxylic (PS-COOH) and amine groups (PS-NH2) were used to determine how surface properties influence the effects of nanoplastics. Results demonstrated the... |
| Tipo: Text |
Palavras-chave: Nanoplastics; Oysters; Gametes; Cellular responses. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00443/55459/56976.pdf |
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| Delaporte, Maryse; Chu, Fu-lin; Langdon, Chris; Moal, Jeanne; Lambert, Christophe; Samain, Jean-francois; Soudant, Philippe. |
| The aim of this study was to assess the effect of dietary eicosapentaenoic acid (20:5n-3) on hemocyte parameters such as hemocyte concentration, phagocytosis, and non-stimulated reactive oxygen species (ROS) production in Pacific oysters Crassostrea gigas, as well on proximate biochemical and fatty acid compositions. One-year-old oysters (C. gigas) were fed T-Isochrysis aff. galbana(T-Iso), which is low in 20:5n-3, either alone or with supplements of a lipid emulsion rich in 20:5n-3 at 1%, 10% or 50% (dry weight of the algal ration) for up to 7 weeks. Changes in gill fatty acid composition demonstrated that the lipid emulsion was well ingested by oysters during the dietary conditioning. Biochemical analysis indicated that oysters fed supplements of 50%... |
| Tipo: Text |
Palavras-chave: Polyunsaturated fatty acids; Immunology; Hemocytes; Eicosapentaenoic acid 20 : 5n 3; Crassostrea gigas; Bivalves. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2604.pdf |
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| Foulon, Valentin; Le Roux, Frederique; Lambert, Christophe; Huvet, Arnaud; Soudant, Philippe; Paul-pont, Ika. |
| Microplastics collected at sea harbour a high diversity of microorganisms including some Vibrio genus members, raising questions about the role of microplastics as a novel ecological niche for potentially pathogenic microorganisms. In the present study we investigated the adhesion dynamics of Vibrio crassostreae on polystyrene microparticles (micro-PS) using electronic and fluorescence microscopy techniques. Micro-PS were incubated with bacteria in different media (Zobell culture medium and artificial seawater) with or without natural marine aggregates. The highest percentage of colonised particles (38-100%) was observed in Zobell culture medium, which may be related to nutrient availability for production of pili and exopolysaccharide adhesion structures.... |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00351/46210/45908.pdf |
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| Paul-pont, Ika; Tallec, Kevin; Gonzalez-fernandez, Carmen; Lambert, Christophe; Vincent, Dorothee; Mazurais, David; Zambonino-infante, Jose-luis; Brotons, Guillaume; Lagarde, Fabienne; Fabioux, Caroline; Soudant, Philippe; Huvet, Arnaud. |
| Marine plastic pollution is a major environmental issue. Given their ubiquitous nature and small dimensions, ingestion of microplastic (MP) and nanoplastic (NP) particles and their subsequent impact on marine life are a growing concern worldwide. Transfers along the trophic chain, including possible translocation, for which the hazards are less understood, are also a major preoccupation. Effects of MP ingestion have been studied on animals through laboratory exposure, showing impacts on feeding activity, reserve depletion and inflammatory responses, with consequences for fitness, notably reproduction. However, most experimental studies have used doses of manufactured virgin microspheres that may not be environmentally realistic. As for most... |
| Tipo: Text |
Palavras-chave: Microplastics; Nanoplastics; Experimental exposure; Impacts; Marine organisms; Physiology. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00449/56088/57621.pdf |
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| Cheize, Marie; Planquette, H.f.; Fitzsimmons, J.n.; Pelleter, Ewan; Sherrell, R.m.; Lambert, Christophe; Bucciarelli, E.; Sarthou, G.; Le Goff, Marion; Liorzou, Celine; Chéron, Sandrine; Viollier, E.; Gayet, Nicolas. |
| A number of trace metals play essential roles in marine ecosystem structure and biological productivity. Until recently, it has been argued that phytoplankton access primarily dissolved iron, while particulate iron was considered a refractory material with little use biologically and limited interaction with the dissolved pool. In order to assess the transfer mechanisms between sediment-sourced particulate trace metals and the dissolved pool, we conducted a 14-month incubation that reacted resuspended sediments with natural seawater, both originating from the Kerguelen area (KEOPS cruises; Southern Ocean), in the dark, and at concentrations replicating natural conditions. Three types of sediments were investigated (named BioSi, BioSi + Ca, and Basalt),... |
| Tipo: Text |
Palavras-chave: Suspended particle dissolution; Sediment; Iron; Manganese; Biogenic silica; Southern Ocean; Kerguelen. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00460/57197/59166.pdf |
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| Moriceau, Brivaela; Iversen, Morten H.; Gallinar, Morgane; Evertsen, Antti-jussi O.; Le Goff, Manon; Beker, Beatriz; Boutorh, Julia; Corvaisier, Rudolph; Coffineau, Nathalie; Donval, Anne; Giering, Sarah L. C.; Koski, Marja; Lambert, Christophe; Lampit, Richard S.; Le Mercier, Alain; Masson, Annick; Stibor, Herwig; Stockenreiter, Maria; De La Rocha, Christina L.. |
| The fraction of net primary production that is exported from the euphotic zone as sinking particulate organic carbon (POC) varies notably through time and from region to region. Phytoplankton containing biominerals, such as silicified diatoms have long been associated with high export fluxes. However, recent reviews point out that the magnitude of export is not controlled by diatoms alone, but determined by the whole plankton community structure. The combined effect of phytoplankton community composition and zooplankton abundance on export flux dynamics, were explored using a set of 12 large outdoor mesocosms. All mesocosms received a daily addition of minor amounts of nitrate and phosphate, while only 6 mesocosms received silicic acid (dSi). This resulted... |
| Tipo: Text |
Palavras-chave: Biogenic silica; POC; Marine snow; Zooplankton; Mesocosm; Bay of Hopavagen; Plankton community; Biological pump. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00469/58029/60433.pdf |
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| González-fernández, Carmen; Toullec, Jordan; Lambert, Christophe; Le Goïc, Nelly; Seoane, Marta; Moriceau, Brivaela; Huvet, Arnaud; Berchel, Mathieu; Vincent, Dorothée; Courcot, Lucie; Soudant, Philippe; Paul-pont, Ika. |
| The potential presence of nanoplastics (NP) in aquatic environments represents a growing concern regarding their possible effects on aquatic organisms. The objective of this study was to assess the impact of polystyrene (PS) amino-modified particles (50 nm PSNH2) on the cellular and metabolic responses of the diatom Chaetoceros neogracile cultures at two essential phases of the growth cycle, i.e. exponential (division) and stationary (storage) phases. Both cultures were exposed for 4 days to low (0.05 μg mL−1) and high (5 μg mL−1) concentrations of PS-NH2. Exposure to NP impaired more drastically the major cellular and physiological parameters during exponential phase than during the stationary phase. Only an increase in ROS production was observed at... |
| Tipo: Text |
Palavras-chave: Nanoplastics; Diatoms; Physiology; Transparent exopolymer particles. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00491/60235/63627.pdf |
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| Roussel, Sabine; Bish, Thomas; Day, Rob; Boudry, Pierre; Huchette, Sylvain; Lambert, Christophe; Lachambre, Sébastien. |
| The domestication of Haliotis tuberculata began recently. During this domestication process, abalone may acquire behavioural and physiological traits to become more adapted to their captive environment. These modifications could be the result of intentional selection on production traits, or of unintentional selection due to specific conditions experienced in the farm environment. In order to study this process, progenies of 3 different broodstock origins were studied. Wild abalone, farmed abalone selected for faster growth at 5% selection pressure and randomly selected farmed abalone were used as broodstock. Farmed abalone were the third generation of abalone resulting from systematic mating between wild and farmed broodstock (either males or females were... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00404/51546/52138.pdf |
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| Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Lambert, Christophe; Quere, Claudie; Miner, Philippe; Choquet, Gwenaelle; Paillard, Christine; Samain, Jean-francois. |
| The impact of diets upon the fatty acid composition of haemocyte polar lipids and consequently upon immune parameters has been tested in the oyster Crassostrea gigas and the clam Ruditapes philippinarum. Oysters and clams were fed each of three cultured algae: Chaetoceros calcitrans, which is rich in 20:5(n-3) and 20:4(n-6) and poor in 22:6(n-3) fatty acids; T-Iso (Isochrysis sp.), which is rich in 22:6(n-3) and deficient in 20:5(n-3) and 20:4(n-6); and Tetraselmis suecica, which is deficient in 22:6(n-3) and contains only small amounts of 20:5(n-3) and 20:4(n-6). Fatty acid composition of haemocyte polar lipids was greatly affected by the diet. Oysters and clams fed C. calcitrans maintained a higher proportion of 20:5(n-3) and 20:4(n-6) in their haemocyte... |
| Tipo: Text |
Palavras-chave: Ruditapes philippinarum; Crassostrea gigas; Polyunsaturated fatty acids; Phagocytosis; Oxidative activity; Haemocyte membrane; Nutrition; Immunology; Bivalve; Immunologie; Bivalve; Ruditapes philippinarum; Crassostrea gigas; Acides gras poly insatures; Phagocytose; Flambee respiratoire; Membrane; Hemocyte; Nutrition. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-354.pdf |
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| Delaporte, Maryse; Moal, Jeanne; Soudant, Philippe; Lambert, Christophe; Pouvreau, Stephane; Enriquez, Martha; Degremont, Lionel; Soletchnik, Patrick; Gagnaire, Beatrice; Ropert, Michel. |
| In the frame of MOREST project, a common biological material, resulting of a mixture of different families produced in experimental hatchery, was reared in two different environmental fields : Normandy and Charente. Concomitantly, a pool was conditioned at the Ifremer Argenton hatchery with three different algae levels : 4%, 8% and 12% of algal dry weight per oyster dry weight. During the experiments, five immune parameters were studied in parallel with survival rate and reproductive status (stages and intensity). Site location, seasonal variations and experimental diet level clearly influenced oyster immune parameters. Haemocyte counts were higher for oysters reared in Normandy than those reared in Charente and in hatchery. Granulocyte percentage was... |
| Tipo: Text |
Palavras-chave: Defense mechanisms; Nutrition; Seasonal variations; Physiology; Crassostrea gigas; Pacific oysters. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/acte-3500.pdf |
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| Guesdon, Stephane; Stachowski-haberkorn, Sabine; Lambert, Christophe; Beker, Beatriz; Brach-papa, Christophe; Auger, Dominique; Bechemin, Christian. |
| This study aimed to describe seasonal variations of phytoplankton abundances in relation to the physical and chemical (nutrients and metals) environment under the influence of freshwater input in the Charente river estuary (Marennes-Oléron bay, France) over three years, from 2011 to 2014. Phytoplankton abundances were determined using microscopy and flow cytometry. Considering high frequency temperature and salinity data, breakpoints in each series led to the identification of two local hydroclimatic periods: the first (2011 and early 2012) being warmer and higher in salinity than the second (from spring 2012 to the beginning of 2014). A multiblock PLS analysis highlighted the significant contribution of the physical environment (temperature, salinity and... |
| Tipo: Text |
Palavras-chave: Hydroclimate; Estuary; Phytoplankton; Size composition; Nutrients; Trace metals. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00349/46005/45674.pdf |
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| Haberkorn, Hansy; Lambert, Christophe; Le Goic, Nelly; Moal, Jeanne; Suquet, Marc; Gueguen, Marielle; Sunila, Inke; Soudant, Philippe. |
| This study assessed the effects of an artificial bloom of the toxin-producing dinoflagellate, Alexandrium minutum, upon nutrition related processes and reproductive output of the Pacific oyster, Crassostrea gigas. Oysters were exposed to A. minutum, Paralytic Shellfish Toxins (PST) producer and compared to a control batch of oysters fed Isochrysis galbana clone Tahitian (T.Iso). The experiment was performed in June 2008, when oysters were found ripe. Several physiological variables of oysters, such as PSTs accumulation, digestive gland and histological observations as well as spermatozoa quality, were measured at the end of the exposure. Results indicate that the digestive gland was greatly impacted upon A. minutum exposure. Monoacylglycerol and... |
| Tipo: Text |
Palavras-chave: Harmful algae; Alexandrium minutum; Oyster; Nutrition-related processes; Reproductive output. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00008/11927/8653.pdf |
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| Haberkorn, Hansy; Lambert, Christophe; Le Goic, Nelly; Gueguen, Marielle; Moal, Jeanne; Palacios, Elena; Lassus, Patrick; Soudant, Philippe. |
| The effects of an artificial bloom of the toxin-producing dinoflagellate, Alexandrium minutum, upon physiological parameters of the Pacific oyster, Crassostrea gigas, were assessed. Diploid and triploid oysters were exposed to cultured A. minutum and compared to control diploid and triploid oysters fed T. Isochrysis. Experiments were repeated twice, in April and mid-May 2007, to investigate effects of maturation stage on oyster responses to A. minutum exposure. Oyster maturation stage, Paralytic Shellfish Toxin (PST) accumulation, as well as several digestive gland and hematological variables, were assessed at the ends of the exposures. In both experiments, triploid oysters accumulated more PSTs (approximately twice) than diploid oysters. Significant... |
| Tipo: Text |
Palavras-chave: Oysters; Ploidy; Physiology; Harmful-algal bloom; Alexandrium minutum; PST accumulation. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00003/11378/7988.pdf |
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| Registros recuperados: 52 | |
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