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Registros recuperados: 7
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Complementation of the Lactococcus lactis secretion machinery with Bacillus subtilis SecDF improves secretion of staphylococcal nuclease Inra
Nouaille, S.; Morello, E.; Cortes Perez, N.; Le Loir, Y.; Commissaire, J.; Gratadoux, J.J.; Poumerol, E.; Gruss, A.; Langella, P..
Unlike Bacillus subtilis and Escherichia coli, the gram-positive lactic acid bacterium Lactococcus lactis does notpossess the SecDF protein, a component of the secretion (Sec) machinery involved in late secretion stages andrequired for the high-capacity protein secretion in B. subtilis. In this study, we complemented the L. lactis Secmachinery with SecDF from B. subtilis and evaluated the effect on the secretion of two forms of staphylococcalnuclease, NucB and NucT, which are efficiently and poorly secreted, respectively. The B. subtilis SecDFencodinggene was tested in L. lactis at different levels. Increased quantities of the precursor and matureforms were observed only at low levels of SecDF and at high NucT production levels. This SecDF...
Tipo: Journal Article Palavras-chave: LACTOCOCCUS LACTIS; BACILLUS SUBTILIS; COMPLEMENTATION; SECRETION.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007bf702808&uri=/notices/prodinra1/2008/02/
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Constitutive delivery of bovine beta-lactoglobulin to the digestive tracts of gnotobiotic mice by engineered Lactobacillus casei Inra
Hazebrouck, S.; Oozeer, R.; Adel-Patient, K.; Langella, P.; Rabot, S.; Wal, J.M.; Corthier, G..
The gut microbiota is critical for maturation of the immune system. Recent evidence suggests that early establishment of lactobacilli in the intestinal microbiota, during neonatal colonization or by probiotic supplementation, could prevent the development of allergic disorders. Postnatal maturation of the gut immune system with allergen-producing lactobacilli colonizing the digestive tract could then affect the development of further allergic sensitization. In this paper, we describe construction of a recombinant Lactobacillus casei strain that can constitutively deliver bovine beta-lactoglobulin (BLG), a major cow's milk allergen, to the guts of gnotobiotic mice. The blg gene was inserted into the L. casei chromosome downstream of an endogenous promoter....
Tipo: Journal Article Palavras-chave: BOVINE BETA-LACTOGLOBULIN; ALLERGEN; PROBIOTIC; INTESTINAL MICROBIOTA; GUT IMMUNE SYSTEM; DIGESTIVE TRACT.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008e9e33f45&uri=/notices/prodinra1/2008/06/
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Heterologous expression of Brucella abortus GroEL heat-shock protein in Lactococcus lactis Inra
Miyoshi, A.; Bermudez Humaran, L.; Ribeiro, L.A.; Le Loir, Y.; Oliveira, S.C.; Langella, P.; Azevedo, V..
Background: Brucella abortus is a facultative intracellular pathogen that mainly infects cattle andhumans. Current vaccines rely on live attenuated strains of B. abortus, which can revert to theirpathogenic status and thus are not totally safe for use in humans. Therefore, the development ofmucosal live vaccines using the food-grade lactic acid bacterium, Lactococcus lactis, as an antigendelivery vector, is an attractive alternative and a safer vaccination strategy against B. abortus. Here,we report the construction of L. lactis strains genetically modified to produce B. abortus GroEL heatshockprotein, a candidate antigen, in two cellular locations, intracellular or secreted.Results: Only the secreted form of GroEL was stably produced in L. lactis,...
Tipo: Journal Article Palavras-chave: LACTOCOCCUS LACTIS; BRUCELLA ABORTUS.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007472cbb64&uri=/notices/prodinra1/2008/02/
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Improvement of bovine beta-lactoglobulin production and secretion by Lactococcus lactis Inra
Nouaille, S.; Bermudez Humaran, L.; Adel-Patient, K.; Commissaire, J.; Gruss, A.; Wal, J.M.; Azevedo, V.; Langella, P.; Chatel, J.M..
The stabilizing effects of staphylococcal nuclease (Nuc) and of a synthetic propeptide (LEISSTCDA, hereafter called LEISS) on the production of a model food allergen, bovine ß-lactoglobulin (BLG), in Lactococcus lactis were investigated. The fusion of Nuc to BLG (Nuc-BLG) results in higher production and secretion of the hybrid protein. When LEISS was fused to BLG, the production of the resulting protein LEISS-BLG was only slightly improved compared to the one obtained with Nuc-BLG. However, the secretion of LEISS-BLG was dramatically enhanced (~10- and 4-fold higher than BLG and Nuc-BLG, respectively). Finally, the fusion of LEISS to Nuc-BLG resulting in the protein LEISS-Nuc-BLG led to the highest production of the hybrid protein, estimated at ~8 µg/ml...
Tipo: Journal Article Palavras-chave: LEISSTCDA; PROTEIN SECRETION; STAPHYLOCCOCAL NUCLEASE; BETA-LACTOGLOBULIN; ALLERGEN.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008d54597d4&uri=/notices/prodinra1/2008/04/
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Secretion of Streptomyces tendae antifungal protein 1 by Lactococcus lactis Inra
Freitas, D.A.; Leclerc, S.; Miyoshi, A.; Oliveira, S.C.; Sommer, P.S.M.; Rodrigues, L.; Correa Junior, A.; Gautier, M.; Langella, P.; Azevedo, V.A.; Le Loir, Y..
Lactococcus lactis, the model lactic acid bacterium, is a good candidate for heterologous protein production in both foodstuffs and the digestive tract. We attempted to produce Streptomyces tendae antifungal protein 1 (Afp1) in L. lactis with the objective of constructing a strain able to limit fungal growth. Since Afp1 activity requires disulfide bond (DSB) formation and since intracellular redox conditions are reportedly unfavorable for DSB formation in prokaryotes, Afp1 was produced as a secreted form. An inducible expression-secretion system was used to drive Afp1 secretion by L. lactis; Afp1 was fused or not with LEISSTCDA, a synthetic propeptide (LEISS) that has been described to be a secretion enhancer. Production of Afp1 alone was not achieved, but...
Tipo: Journal Article Palavras-chave: LACTOCOCCUS LACTIS; ANTIFUNGAL PROTEIN; SECRETION; STREPTOMYCES TENDAE ANTIFUNGAL PROTEIN 1.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20074cfb9bfe&uri=/notices/prodinra1/2007/02/
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Testing of a whole genome PCR scanning approach to identify genomic variability in four different species of lactic acid bacteria Inra
Ben Zakour, N.; Grimaldi, C.; Gautier, M.; Langella, P.; Azevedo, V.; Maguin, E.; Le Loir, Y..
Genomes can be markedly heterogeneous in conspecific bacterial strains. Genome sequences can be used to analyze genome plasticity via a PCR2 (plasticity of chromosome revealed by PCR) approach. Small-sized chromosomes can indeed be fully amplified by long-range PCR with a set of primers designed using a reference strain and then applied to several other strains. Analysis of the resulting patterns can reveal genome plasticity. GenoFrag, a software package for the design of primers optimized for PCR2 [N. Ben Zakour, M. Gautier, R. Andonov, D. Lavenier, M.F. Cochet, P. Veber, A. Sorokin, Y. Le Loir, GenoFrag: Software to design primers optimized for whole genome scanning by long-range PCR amplification, Nucleic Acids Res. 32 (2004) 17–24] was developed for...
Tipo: Journal Article Palavras-chave: TYPAGE DES MICROORGANISMES GENOME VARIABILITY; FOOD GRADE BACTERIA; LONG-RANGE PCR; MICROORGANISMS TYPING.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007143e7e93&uri=/notices/prodinra1/2008/01/
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Use of native lactococci as vehicles for delivery of DNA into mammalian epithelial cells Inra
Dellaretti Guimarães, V.; Innocentin, S.; Lefèvre, F.; Azevedo, V.; Wal, J.M.; Langella, P.; Chatel, J.M..
The use of the food-grade bacterium Lactococcus lactis as a DNA delivery vehicle at the mucosal level is an attractive DNA vaccination strategy. Previous experiments showed that recombinant L. lactis expressing the Listeria monocytogenes inlA gene can deliver a functional gene into mammalian cells. Here, we explored the potential use of noninvasive L. lactis strains as a DNA delivery vehicle. We constructed two Escherichia coli-L. lactis shuttle plasmids, pLIG:BLG1 and pLIG:BLG2, containing a eukaryotic expression cassette with the cDNA of bovine beta-lactoglobulin (BLG). The greatest BLG expression after transfection of Cos-7 cells was obtained with pLIG:BLG1, which was then used to transform L. lactis MG1363. The resulting L. lactis strain...
Tipo: Journal Article Palavras-chave: VACCINATION; GENE TRANSFER TECHNIQUE; CACO-2 CELLS; BOVINE BETA-LACTOGLOBULIN; DNA VACCINE DELIVERY; MAMMALIAN EPITHELIUM CELL.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200819139252&uri=/notices/prodinra1/2008/06/
Registros recuperados: 7
Primeira ... 1 ... Última
 

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