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An update on equine laminitis Ciência Rural
Laskoski,Luciane Maria; Valadão,Carlos Augusto Araújo; Dittrich,Rosangela Locatelli; Deconto,Ivan; Faleiros,Rafael Resende.
ABSTRACT: Laminitis is a severe podal affection, which pathophysiology remains partially renowned. Ischemic, enzymatic, metabolic and inflammatory mechanisms are connected to the development of laminar lesions. However, few therapeutic measures are effective to prevent or control the severity of acute laminitis and its prodromal stage, which often determines serious complications such as rotation and/or sinking of the distal phalanx and even the loss of hoof. The purpose of this study is to compile the actual knowledge in respect to the pathophysiology and treatment of equine laminitis.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Horse; Pathophysiology; Treatment; Podal disease.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300547
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Anticoagulants on yield of bone marrow-derived mononuclear cells harvested from dogs Ciência Rural
Brito,Harald Fernando Vicente de; Fernandes-Filho,Tobias; Brandão,Fernanda de Lima; Montaño,Patrícia Yukiko; Laskoski,Luciane Maria; Locatelli-Dittrich,Rosangela.
Cell therapy with bone marrow-derived mononuclear cells is an alternative to therapy with mesenchymal stem cell cultures. The aim of the present research was the comparison of the yield of bone marrow-derived mononuclear cells harvested from dogs with two different anticoagulants. Bone marrow was harvested from the iliac crest of five healthy dogs aged between 15 and 30 months, and the effect of two anticoagulant solutions, CPDA-1 (citrate phosphate dextrose adenine-1) and heparin, on the isolation of mononuclear cells was compared. Mononuclear cells were isolated in a density gradient and stained for CD9 and CD44 for characterization by flow cytometry. Means were compared using Student's paired t-test. Samples harvested with CPDA-1 yielded an average of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mesenchymal stem cells; Multipotent stem cell; Immunophenotyping.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001102044
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Atresia anal, fístula uretrorretal congênita, bolsa escrotal acessória e pseudo-hermafroditismo em bezerro mestiço Ciência Rural
Rocha,Thaís Gomes; Laskoski,Luciane Maria; Lopes,Maristela de Cássia Seudo; Berlingieri,Maria Augusta; Magalhães,Geórgia Modé; Alessi,Antonio Carlos.
Neste trabalho, é descrito o caso de um bezerro mestiço recém-nascido que apresentava atresia anal tipo 2, fístula uretrorretal congênita, bolsa escrotal bífida e pseudo-hermafroditismo masculino. O principal sinal clínico era a eliminação de fezes por meio do óstio prepucial, uma apresentação incomum em casos de fístula uretrorretal em animais machos. Apesar de o quadro de atresia anal ser relativamente comum nessa espécie, os outros defeitos congênitos encontrados são pouco frequentes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Defeitos congênitos; Atresia anal; Escroto bífido; Diferenciação sexual; Bovino.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000500039
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Expression of matrix metalloproteases-2 and -9 in horse hoof laminae after intestinal obstruction, with or without Hydrocortisone treatment Ciência Rural
Laskoski,Luciane Maria; Valadão,Carlos Augusto Araújo; Vasconcelos,Rosemeri de Oliveira; Ferrucci,Danilo; Silva,Juliete Aparecida Francisco; Machado,Dagmar Ruth Stach; Doria,Renata Gebara Sampaio; Mendonça,Fabio de Sousa.
Twenty horses were used in the experiment, for composed control group, (Cg) instrumented group, (Ig;without intestinal obstruction), treated group (Tg;submitted to intestinal obstruction and hydrocortisone treatment) and non-treated group (Ntg;submitted to intestinal obstruction without treatment). Immunohistochemistry and zymography techniques were used for researches on MMPs 2 and 9 in horse hoof laminae. There was an increase in the expression of MMP-2 in animals of Tg and Ntg. MMP-9 increased on animals from groups Ntg and Ig, however there was no rise of this MMP on the Tg when compared to the other groups in the immunohistochemistry analysis. Based on the results, it was observed that the intestinal injury caused by enterotomy and intestinal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Laminitis; Metalloprotease; Horse; Zymography; Immunohistochemistry.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100012
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Pesquisa investiga fisiopatogenia da laminite em equinos Ciência Rural
Laskoski,Luciane Maria.
Tipo: Info:eu-repo/semantics/other
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013010200002
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