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| Leal,Nilma Cintra; Sobreira,Marise; Leal,Tereza Cristina Arcanjo; Almeida,Alzira Maria Paiva de. |
| Yersinia pestis, the etiologic agent of plague, harbors three well-characterized plasmids: pFra (90-110kb), pYV (70 kb) and pPst (9.5 kb). Furthermore, some extra-cryptic DNA bands have been observed in a number of wild strains from several foci of the world. Additional bands have also been reported in Brazilian strains. Looking for any relationship among these cryptic DNA bands and the three-prototypical plasmids, we analyzed twelve strains displaying different plasmid content. The DNA bands were hybridized by southern blot with probes directed at the genes caf1, lcrV and pla located respectively on the plasmids pFra, pYV and pPst. The probes were constructed by PCR amplification and labeled with digoxigenin. The Pla probe hybridized with its target... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Y. pestis; Plasmids; Probes; Virulence genes. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100006 |
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| Bezerra,Gilberto Silva Nunes; Barbosa Júnior,Walter Lins; Silva,Elis Dionísio da; Leal,Nilma Cintra; Medeiros,Zulma Maria de. |
| ABSTRACT Visceral leishmaniasis is a serious and debilitating infection with high fatality rate in tropical and subtropical countries. As clinical symptoms of visceral leishmaniasis are not so specific, confirmatory diagnostic methods with high sensitivity and specificity are needed. Noninvasive methods have been developed using urine as a clinical sample for visceral leishmaniasis diagnosis. In fact, there is a clear correlation between kidney impairment and Leishmania DNA in urine. However, it has been proved that Leishmania nucleic acid may also be isolated from patients without any sign of renal involvement. Even though urine has become a promissing biological sample, it is still not widely used due to several issues, such as (i) incomprehension of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Visceral leishmaniasis; Renal involvement; Diagnosis; Urine sample; DNA extraction. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000200111 |
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