|
|
|
|
|
| Leme Junior,Jorge; Malavolta,E.. |
| The photometric determination of ascorbic acid with the "E. E. L. portable colorimeter" can be carried" out rapid and conveniently using either 3% HPO3 or 0,4% (COOH) 2 as protective agent. The standards would contain from 2 to 20 micrograms of ascorbic acid per ml of metaphosphoric or oxalic acid solutions. We mix 10 ml of these solutions with 3 ml of the adequate citrate buffer solutions, and we pipet 5 ml of the resulting mixture to a matched test tube containing 5 ml of sodium - 2,6 - dichlorobenzenoneindophenol (80 mg per liter); then we shake well and after 15 seconds the extintion is read using green filter. The readings are subtracted from the blank one. Designating the differences by x and the concentrations of ascorbic acid/ml in the standards by... |
| Tipo: Info:eu-repo/semantics/article |
|
| Ano: 1950 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0071-12761950000100016 |
| |
|
|
|
