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Lew,Betina Joyce; Oliveira,Mauro Dal Secco de; Freitas Júnior,José Esler de; Carvalho,Marina Vieira de; Rêgo,Aníbal Coutinho do; Rennó,Francisco Palma. |
The objective of this study was to determine the effects of dietary energy and recombinant bovine somatotropin (bST) injection to identify genes that might control mammogenesis. Total RNA was extracted from the parenchymal tissue of 32 heifers randomly assigned to one of four treatments: two diets (a standard diet and a high energy, high protein diet), each with or without bST. To perform microarray experiments, RNA samples were pooled (2 animals/pool) before reverse transcription and labeling with Cy3 or Cy5. A 4-node loop design was used to examine the differential gene expression among treatments using a bovine-specific cDNA microarray (National Bovine Functional Genomics Consortium Library, NBFGC) containing 18,263 unique expressed sequence tags (EST).... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene regulation; Mammary gland development; Microarray; Nutrition; Somatotropin. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000700008 |
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