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Registros recuperados: 8
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A novel expression vector for the secretion of abaecin in Bacillus subtilis BJM
Li,Li; Mu,Lan; Wang,Xiaojuan; Yu,Jingfeng; Hu,Ruiping; Li,Zhen.
ABSTRACT This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter Pglv, and a mature beta-glucanase signal peptide. Also, a B. subtilis expression system was constructed. The recombinant abaecin gene was expressed and purified as a recombinant protein in the culture supernatant. The purified abaecin did not inhibit the growth of Escherichia coli strain K88. Cecropin A and hymenoptaecin exhibited potent bactericidal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abaecin; Antimicrobial peptides; Bacillus subtilis; Expression.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400809
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Antibacterial activity of α-terpineol may induce morphostructural alterations in Escherichia coli BJM
Li,Li; Shi,Chaofeng; Yin,Zhongqiong; Jia,Renyong; Peng,Lianci; Kang,Shuai; Li,Zhengwen.
The antibacterial effect of α-terpineol from Cinnamomum longepaniculatum (Gamble) N. Chao leaf essential oils were studied with special reference to the mechanism of inhibiting the standard strain of Escherichia coli (CMCC (B) 44102) growth at ultrastructural level. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) and time-kill curves of α-terpineol were determined; Escherichia coli was treated with α-terpineol and observed under a transmission electron microscope. The MIC and MBC values of α-terpineol were all 0.78 µL/mL, and time-kill curves showed the concentration-dependent. Under the transmission electron microscopy (TEM), Escherichia coli exposed to MIC levels of α-terpineol exhibited decreased cell size and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Α-terpineol; Escherichia coli; Antibacterial activity; TEM.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400035
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Chinese pregnant women in their third trimester are more susceptible to HEV infection BJID
Huang,Fen; Wang,Jue; Yang,Chenchen; Long,Feiyan; Li,Yunlong; Li,Li; Jing,Shenrong; Wang,Huixuan.
Tipo: Info:eu-repo/semantics/other
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600672
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Low seroprevalence of hepatitis E virus infection in pregnant women in Yunnan, China BJID
Huang,Fen; Ma,Tianwu; Li,Li; Zeng,Weikun; Jing,Shenrong.
Tipo: Info:eu-repo/semantics/other
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600017
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RestrictionDigest: A powerful Perl module for simulating genomic restriction digests Electron. J. Biotechnol.
Wang,Jinpeng; Li,Li; Qi,Haigang; Du,Xuedi; Zhang,Guofan.
Background: Reduced-representation sequencing technology is widely used in genotyping for its economical and efficient features. A popular way to construct the reduced-representation sequencing libraries is to digest the genomic DNA with restriction enzymes. A key factor of this method is to determine the restriction enzyme(s). But there are few computer programs which can evaluate the usability of restriction enzymes in reduced-representation sequencing. SimRAD is an R package which can simulate the digestion of DNA sequence by restriction enzymes and return enzyme loci number as well as fragment number. But for linkage mapping analysis, enzyme loci distribution is also an important factor to evaluate the enzyme. For phylogenetic studies, comparison of...
Tipo: Journal article Palavras-chave: Genotyping; Reduced-representation libraries; Reduced-representation sequencing.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300003
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Serum regucalcin is a useful indicator of liver injury severity in patients with hepatitis B virus-related liver diseases BJMBR
Wei,Xinhuan; Yu,Haibin; Zhao,Peng; Xie,Li; Li,Li; Zhang,Jing.
Regucalcin is a soluble protein that is principally expressed in hepatocytes. Studies of regucalcin have mainly been conducted in animals due to a lack of commercially available kits. We aimed to develop an enzyme-linked immunosorbent assay (ELISA) to quantify serum regucalcin in patients with hepatitis B virus (HBV)-related disease. High-titer monoclonal antibodies and a polyclonal antibody to regucalcin were produced, a double-antibody sandwich ELISA method was established, and serum regucalcin was determined in 47 chronic hepatitis B (CHB) patients, 91 HBV-related acute-on-chronic liver failure (HBV-ACLF) patients, and 33 healthy controls. The ELISA demonstrated an appropriate linear range, and high levels of reproducibility, sensitivity, specificity,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Regucalcin; Liver injury; Chronic hepatitis B infection; ELISA; Liver failure.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000605
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The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer Electron. J. Biotechnol.
Li,Li; Zhong,Cai-hong; Bian,Yin-bing.
Background: For the crossbreeding of Auricularia auricula-judae, selecting the appropriated parents in hybridization is very important. However, the classification and diversity analysis of A. auricula-judae has been equivocal, due to the similarity of the fruiting body morphology and its susceptibility to environmental influences. For this purpose, the molecular diversity of 32 A. auricula-judae commercial cultivars in China was analyzed by using the nuclear ribosomal DNA intergenic spacer. Results: The complete nuclear rDNA gene complex of A. auricula-judae isolate is 11,210 bp long, and contains the 18S, 5.8S, and 28S rRNA gene as well as the ITS and IGS regions. Based on the sequence data, four more effective primer combinations for the IGS region of...
Tipo: Journal article Palavras-chave: Gremplasm resource Genetic polymorphism Strain identification Ribosomal RNA genes.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100005
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The TLR7 agonist Imiquimod promote the immunogenicity of msenchymal stem cells Biol. Res.
Zhang,Li; Liu,Dan; Pu,Dan; Wang,Yanwen; Li,Li; He,Yanqi; Li,Yalun; Li,Lei; Li,Weimin.
BACKGROUND: Mesenchymal stem cells (MSCs) are considered the best candidate in stem cells therapy due to their multipotent differentiation ability, low expression of co-stimulatory molecules (CD80, CD86, CD34 and HLA-II) and immunosuppression effects on in vivo immune responses. MSCs were now widely used in clinical trials but received no encourage results. The major problem was the fate of engrafted MSCs in vivo could not be defined. Some studies indicated that MSCs could induce immune response and result in the damage and rejection of MSCs. As toll like receptors (TLRs) are important in inducing of immune responses, in this study we study the role of TLR7 in mediating the immune status of MSCs isolated from umbilical cord. RESULTS: Our results indicated...
Tipo: Journal article Palavras-chave: Mesenchymal stem cells; Umbilical cord; Toll like receptor 7; Immunogenicity; Pro-inflammatory molecules.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100006
Registros recuperados: 8
Primeira ... 1 ... Última
 

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