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Registros recuperados: 8
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Acetylcholinesterase enzyme activity in carp brain and muscle after acute exposure to diafuran Scientia Agricola
Golombieski,Jaqueline Ineu; Marchesan,Enio; Camargo,Edinalvo Rabaioli; Salbego,Joseânia; Baumart,Joele Schmitt; Loro,Vania Lucia; Machado,Sérgio Luiz de Oliveira; Zanella,Renato; Baldisserotto,Bernardo.
Sublethal adverse effects may result from exposure of aquatic organisms to insecticides at environmentally relevant concentrations. Fingerlings of the common carp (Cyprinus carpio, Linnaeus, 1758), grass carp (Ctenopharyngodon idella, Valenciennes, 1844), and bighead carp (Aristichthys nobilis, Richardson, 1845) were exposed to diafuran, an insecticide widely used during rice cultivation in Southern Brazil. The aim of this study was to verify the relationship between the lethal concentration (LC50) of diafuran and the acetylcholinesterase (AChE) activity in brain and muscle tissues of these species as a possible early biomarker of exposure to this insecticide. LC50 was determined for fish exposed to diafuran concentrations during 96 h (short term): common...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyprinus carpio; Ctenopharyngodon idella; Aristichthys nobilis; Pesticide; Lethal concentration.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000400003
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Anesthesia of silver catfish with eugenol: time of induction, cortisol response and sensory analysis of fillet Ciência Rural
Cunha,Mauro Alves da; Zeppenfeld,Carla Cristina; Garcia,Luciano de Oliveira; Loro,Vania Lucia; Fonseca,Milene Braga da; Emanuelli,Tatiana; Veeck,Ana Paula de Lima; Copatti,Carlos Eduardo; Baldisserotto,Bernardo.
The aim of this study was to identify the time of anesthetic induction and recovery of silver catfish (Rhamdia quelen) exposed to eugenol. It was also determined the efficacy of the anesthetic as a stress reducing agent and performed a sensory analysis of the fillets from fish exposed to this substance. The silver catfish were exposed to air for 1min to carry out biometry, and blood was collected at 0, 1 and 4 hours later. Eugenol can be used in the range of 20-50mg L-1 for anesthetic induction in silver catfish, and recovery time from anesthesia was not affected by eugenol concentration. The control group showed significantly higher cortisol levels 4 hours after biometry than at time zero. Fish anesthetized with eugenol (50mg L-1) presented significantly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clove oil; Rhamdia quelen; Sedation; Stress; Taste.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001000009
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Changes in oxidative markers, endogenous antioxidants and activity of the enzyme acetylcholinesterase in farmers exposed to agricultural pesticides - a pilot study Ciência Rural
Murussi,Camila; Horn,Roberta Cattaneo; Santi,Adriana; Clasen,Barbara Estevão; Reis,Gabriel; Souza,Daniela; Bortolotto,Josiane Woutheres; Manfio,Candida Elisa; Loro,Vania Lucia.
Agricultural practices are directly related to the use of pesticides, which indiscriminately and without due care may contribute to the occurrence of numerous intoxications. Several studies have demonstrated the relationship of certain pesticides and the occurrence of oxidative stress and therefore, in recent years have developed methods of analysis of several biomarkers of cellular damage that can be measured and quantified. In this context, the objective of this study was to evaluate the possible changes in biochemical markers: glutamic aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, urea, total protein, and oxidative markers such as lipid peroxidation, damage to proteins and the activity of the enzymes acetylcholinesterase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rarmers; Oxidative stress; TBARS; Protein carbonyls.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000701186
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Different feeding habits influence the activity of digestive enzymes in freshwater fish Ciência Rural
Gioda,Carolina Rosa; Pretto,Alexandra; Freitas,Carine de Souza; Leitemperger,Jossiele; Loro,Vania Lucia; Lazzari,Rafael; Lissner,Leandro Ademar; Baldisserotto,Bernardo; Salbego,Joseânia.
ABSTRACT: The aim of this study was to verify the activity of some digestive enzymes in four fish species with different feeding habits. Knowledge of these enzymatic activities can help us to understand the species' digestive processes. The species chosen for this study were Ctenopharyngodon idella (herbivore), Rhamdia quelen (omnivore), Leporinus obtusidens (omnivore) and Hoplias malabaricus (carnivore). The digestive tract of these species was divided into four portions to estimate enzymatic activity: stomach, anterior, mid and posterior intestine. Ctenopharyngodon idella had the highest amylase and maltase activities in all portions of the gut, followed by L. obtusidens . The highest trypsin activity was observed in all gut portions of H. malabaricus,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Freshwater fishes; Feeding habits; Enzymatic activity; Amylase; Alkaline protease; Maltase; Trypsin.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300406
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Glyphosate on digestive enzymes activity in piava (Leporinus obtusidens) Ciência Rural
Salbego,Joseânia; Pretto,Alexandra; Silva,Vera Maria Machado da; Loro,Vania Lucia; Lazzari,Rafael; Gioda,Carolina Rosa; Baldisserotto,Bernardo.
The effects of glyphosate, a nonselective herbicide (1.0 or 5.0mg L-1) on digestive enzymes activity (stomach and intestine) were evaluated in juveniles of piava (Leporinus obtusidens) after 90 days of exposure. The activity of acid protease, trypsin, chymotrypsin and amylase increased with the increase of glyphosate concentration. These results indicate that glyphosate affects digestive enzyme activities in this species, and may be an indicator of poor nutrient availability when fish survive in herbicide-contaminated water.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Herbicide; Acid protease; Amylase; Trypsin.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000901603
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Glyphosate-based herbicide affects biochemical parameters in Rhamdia quelen (Quoy & Gaimard, 1824 and) Leporinus obtusidens (Valenciennes, 1837) Neotropical Ichthyology
Loro,Vania Lucia; Glusczak,Lissandra; Moraes,Bibiana S.; Leal,Claudio A. M.; Menezes,Charlene; Murussi,Camila R.; Leitemperger,Jossiele; Schetinger,Maria Rosa C.; Morsch,Vera Maria.
Rhamdia quelen (silver catfish) and Leporinus obtusidens (piava) were exposed to a commercial formulation Roundup(r), a glyphosate-based herbicide at concentrations of 0.2 or 0.4 mg/L for 96 h. The effects of the herbicide were analyzed on the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and glucose in plasma, glucose and protein in the mucus layer, nucleotide hydrolysis in the brain, and protein carbonyl in the liver. The parameters were chosen, owing to a lack of information concerning integrated analysis, considering oxidative damage parameters, liver damage, and effects on the mucus layer composition and triphosphate diphosphohydrolase (NTPDase) activities. Plasmatic glucose levels were reduced in both species, whereas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Mucus; Nucleotidases; Plasma; Protein carbonyl.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000100229
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Protein sources and digestive enzyme activities in jundiá (Rhamdia quelen) Scientia Agricola
Lazzari,Rafael; Radünz Neto,João; Pedron,Fabio de Araújo; Loro,Vania Lucia; Pretto,Alexandra; Gioda,Carolina Rosa.
Digestive enzymes activity influence feed utilization by fish, and its understanding is important to optimize diet formulation. This study reports the digestive enzyme activities of jundiá juveniles fed diets with protein sources. Fish were fed six experimental diets for 90 days: MBY (meat and bone meal + sugar cane yeast), SY (soybean meal + sugar cane yeast), S (soybean meal), MBS (meat and bone meal + soybean meal), FY (fish meal + sugar cane yeast) and FS (fish meal + soybean meal), and then sampled every 30 days and assayed in two intestine sections for digestive enzymes - trypsin, chymotrypsin and amylase - activities; gastric protease was assayed in the stomach. Digestive and hepatosomatic index, intestinal quotient, digestive tract length and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestion; Nutrition; Soybean meal; Meat and bone meal; Fish meal.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000300002
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Spatial and temporal biomarkers responses of Astyanax jacuhiensis (Cope, 1894)(Characiformes: Characidae) from the middle rio Uruguai, Brazil Neotropical Ichthyology
Loro,Vania Lucia; Murussi,Camila; Menezes,Charlene; Leitemperger,Jossiele; Severo,Eduardo; Guerra,Luciana; Costa,Maiara; Perazzo,Giselle Xavier; Zanella,Renato.
Due to intense agricultural activity in the rio Uruguai (South Brazil), there is the potential for aquatic contamination by agrochemicals. In this region, there are many reservoirs to meet the water demand for rice fields, forming lentic environments. In line with this information, the aim of this study was to show a comparative analysis of some biomarkers, such as lipid peroxidation (TBARS), gluthatione S-transferase (GST), non-protein thiols (NPSH), amino acids (AA) and piscine micronucleus tests (MNE) in Astyanax jacuhiensis from lentic and lotic environments in the middle rio Uruguai region, comparing warm and cold seasons. Eight pesticides were found in water samples, with propoxur having the highest concentration found in both environments and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomonitoring; Lotic and lentic environments; Pampa; Pesticides.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300569
Registros recuperados: 8
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