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Antifungal susceptibility profile of Trichosporon isolates: correlation between CLSI and etest methodologies BJM
Lemes,Raquel M.L.; Lyon,Juliana P.; Moreira,Leonardo M.; Resende,Maria Aparecida de.
The aim of the present study was to evaluate the antifungal susceptibility profile of Trichosporon species isolated from different sources employing the Clinical and Laboratory Standards Institute (CLSI) method and E-test method. Thirty-four isolates of Trichosporon spp. and six CBS reference samples were tested for their susceptibility to Amphotericin B, 5-flucytosine, Fluconazole, Itraconazole, Voriconazole and Terbinafine. All species showed high Minimun Inhibitory Concentrations (MIC) for Itraconazole and susceptibility to Fluconazole, The comparison among the results obtained by the CLSI method and E-test revealed larger discrepancies among 5-flucytosine and Itraconazole. The present work provides epidemiological data that could influence therapeutic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichosporon spp; Antifungal drugs; CLSI; E-test.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200008
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Assessment of the genetic risks of a metallic alloy used in medical implants Genet. Mol. Biol.
Gomes,Cristiano C.; Moreira,Leonardo M.; Santos,Vanessa J.S.V.; Ramos,Alfeu S.; Lyon,Juliana P.; Soares,Cristina P.; Santos,Fabio V..
The use of artificial implants provides a palliative or permanent solution for individuals who have lost some bodily function through disease, an accident or natural wear. This functional loss can be compensated for by the use of medical devices produced from special biomaterials. Titanium alloy (Ti-6Al-4V) is a well-established primary metallic biomaterial for orthopedic implants, but the toxicity of the chemical components of this alloy has become an issue of concern. In this work, we used the MTT assay and micronucleus assay to examine the cytotoxicity and genotoxicity, respectively, of an extract obtained from this alloy. The MTT assay indicated that the mitochondrial activity and cell viability of CHO-K1 cells were unaffected by exposure to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biomaterial; Cytotoxicity; Micronucleus; Mutagenicity; Ti-6Al-4V.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100020
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Candida albicans: genotyping methods and clade related phenotypic characteristics BJM
Lyon,Juliana P.; Moraes,Karen C.M.; Moreira,Leonardo M.; Aimbire,Flávio; Resende,Maria Aparecida de.
Several molecular methods, such as Southern blotting hybridization, Multilocus Sequence Typing, and DNA microsatellite analysis, have been employed to genotype Candida albicans. The genotype analysis allows to group strains in clades, that is, a group composed of one ancestor and its descendants. These genotype studies demonstrate that clades distribution is influenced by geographic area as well as that antifungal resistance is associated with particular clades. These findings suggested that C. albicans reproduces mainly in a clonal manner, with certain degree of DNA microevolution. Additionally, virulence factors and site of isolation have also been associated with clade specificity. The present article is a brief review about the methods used for Candida...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Clonal reproduction; Microevolution; Genotyping systems; Phenotypic characteristics.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400003
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