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MARTINS, F. A.; CARNEIRO, P. C. S.; GUIMARAES, C. T.; MAGALHAES, J. V. D.; CARNEIRO, J. E. S.; CRUZ, C. D.. |
The lack of informativity of samples from heterozygotic individuals is one of the hindrances in the mapping of quantitative trait loci of outbred populations, since it is not normally possible to identify the origin of each allele. One way to include these individuals in analyses would be to genotype their endosperm, considering that heterozygote (Aa) has AAa endosperm, when the female genitor donates the A or a allele, respectivelly. We used semiquantitative polymerase chain reaction to determine allele dosages in DNA mixtures, by simulating the observed conditions for endospermic tissue. Semiquantitative polymerase chain reaction on agarose gels, along with regression analysis, allowed differentiation of the samples according to the amount of DNA. This... |
Tipo: Artigo de periódico |
Palavras-chave: Milho; Zea mays; Genética vegetal. |
Ano: 2009 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/513749 |
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GUIMARAES, C. T.; MAGALHAES, J. V. D.; JARDIM, S. N.; ALMEIDA, R. V.; MARON, L.; PARENTONI, S. N.; ALVES, V. M. C.; LANA, U. G. D. P.; GAMA, E. E. G.; HOEKENGA, O.; PAIVA, E.; KOCHIAN, L. V.. |
Tipo: Parte de livro |
Palavras-chave: Milho; Alumínio; Zea Mays.. |
Ano: 2009 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/492005 |
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GUIMARAES, C. T.; MAGALHAES, J. V. D.; JARDIM, S. N.; ALMEIDA, R. V.; HOEKENGA, O.; PAIVA, E.; ALVES, V. M. C.; MARON, L. G.; PARENTONI, S. N.; GAMA, E. E. G.; KOCHIAN, L. V.. |
Tipo: Parte de livro |
Palavras-chave: Toxicidade.; Milho.. |
Ano: 2009 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/514053 |
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SCHAFFERT, R. E.; SILVA, L. A.; ALVES, V. M. C.; CARVALHO, G. A.; MAGALHAES, J. V. D.. |
AltSB, a major gene of the Multidrug and Toxic Compound Extrusion (Mate) family confers tolerance to aluminum toxicity in sorghum. This gene is a transporter gene that is responsible for the exudation of citric acid in the presence of toxic level of aluminum in the soil. The citrate complexes with the toxic aluminum forming a nontoxic compound. During the past several years, isogenic sorghum breeding lines, both cytoplasmic male-sterile lines (A and B-lines) and fertility restoring pollinator lines (R-lines) for this AlTSB gene have been developed at Embrapa Maize and Sorghum. These isogenic lines for AlTSB were used to develop sixteen isogenic sorghum hybrids with zero, one and two alleles for tolerance to aluminum toxicity. These sixteen hybrids are... |
Tipo: Parte de livro |
Palavras-chave: Toxicidade.; Sorgo; Aluminio.. |
Ano: 2009 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/513834 |
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GUIMARAES, C. T.; MAGALHAES, J. V. D.; JARDIM, S. N.; ALMEIDA, R. V.; MARON, L. G.; PAIVA, E.; ALVES, V. M. C.; VIANA, J. M. S.; HOEKENGA, O.; PARENTONI, S. N.; KOCHIAN, L. V.. |
Tipo: Parte de livro |
Palavras-chave: Tolerância; Milho; Zea mays; Marcador molecular; Alumínio. |
Ano: 2009 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/514048 |
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