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| Registros recuperados: 30 | |
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| ARDISSON-ARAÚJO, D. M. P.; PEREIRA, B. T.; MELO, F. L.; RIBEIRO, B. M.; BÁO, S. N.; ZANOTTO, P. M. de A.; MOSCARDI, F.; KITAJIMA, E. W.; SOSA-GÓMEZ, D. R.; WOLFF, J. L. C.. |
| A betabaculovirus (DisaGV) was isolated from Diatraea saccharalis (Lepidoptera: Crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in Brazil. The complete genome sequence of DisaGV was determined using the 454-pyrosequencing method. The genome was 98,392 bp long, which makes it the smallest lepidopteran-infecting baculovirus sequenced to date. It had a G + C content of 29.7 % encoding 125 putative open reading frames (ORF). All the 37 baculovirus core genes and a set of 19 betabaculovirus-specific genes were found. A group of 13 putative genes was not found in any other baculovirus genome sequenced so far. A phylogenetic analysis indicated that DisaGV is a member of Betabaculovirus genus and that it is a sister... |
| Tipo: Separatas |
Palavras-chave: Cana.; Genoma.; Betabaculovirus; Lepidoptera.. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1059049 |
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| SILVA, L. A. da; OLIVEIRA, A. S.; MELO, F. L.; ARDISSON-ARAÚJO, D. M. P.; RESENDE, F. V.; RESENDE, R. O.; RIBEIRO, B. M.. |
| In this study, we identified viruses present in different garlic cultivars from the germplasm collection of EMBRAPA Hortaliças, Brazil. The majority of the viruses found in these samples were previously reported, except for a new virus putatively classified as a member of new genus in the family Betaflexiviridae (order Tymovirales). |
| Tipo: Artigo de periódico |
Palavras-chave: Alho; Vírus; Allium Sativum; Garlic; Betaflexiviridae. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1107803 |
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| SILVA, K. N.; SOUZA, J. M.; MELO, F. L.; NAGATA, T.; SILVA, M. S.; ORILIO, A. F.; FERNANDES, C. D.; JANK, L.; SANTOS, M. F.; VERZIGNASSI, J. R.; FRAGOSO, R. da R.; DESSAUNE, S. N.; RESENDE, R. O.. |
| Em área experimental da Embrapa Gado de Corte, Campo Grande-MS, genótipos de espécies de Panicum, Brachiaria e Stylosanthes têm exibido sintomas típicos de infecção viral, como mosaico nas folhas e redução do crescimento da parte aérea, cuja etiologia ainda não estava esclarecida. Fontes de resistência são as principais formas de controle da doença. Objetivou-se, neste trabalho, identificar o(s) vírus agente(s) da doença, bem como fontes de resistência de P. maximum à mesma. Folhas sintomáticas foram maceradas em tampão PBS e o extrato foliar foi centrifugado e filtrado. O RNA viral foi extraído utilizando-se RNeasy Mini Kit, de acordo com as instruções do fabricante. As amostras de RNA foram concentradas e sequenciadas na Macrogen inc. (Korea) usando-se a... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Diversidade viral.; Graminea; Genoma; Filogenia; Estilosante.. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1066082 |
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| OLIVEIRA, A. S. de; MELO, F. L.; INOUE NAGATA, A. K.; NAGATA, T.; KITAJIMA, E. W.; RESENDE, R. O.. |
| Tospoviruses (Genus Tospovirus, Family Bunyaviridae) are phytopathogens responsible for significant worldwide crop losses. They have a tripartite negative and ambisense RNA genome segments, termed S (Small), M (Medium) and L (Large) RNA. The vector-transmission is mediated by thrips in a circulative-propagative manner. For new tospovirus species acceptance, several analyses are needed, e.g., the determination of the viral protein sequences for enlightenment of their evolutionary history. |
| Tipo: Separatas |
Palavras-chave: Análise serológica; Sequencia genômica; Bean necrotic mosaic virus; SVNaV; BeNMV; Virus; Genoma; Linhagem; Sintoma; Tospovirus. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/934717 |
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| NAKASU, E. Y. T.; MELO, F. L.; NAGATA, T.; MICHEREFF FILHO, M.; SOUZA, J. O.; RIBEIRO, B. M.; RIBEIRO, S. G.; LACORTE, C.; PEREIRA, J. L.; INOUE-NAGATA, A. K.. |
| Tipo: Parte de livro |
Palavras-chave: Mosca branca; Controle biológico; RNA; Virus. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1031015 |
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| NAKASU, E. Y. T.; MELO, F. L.; NAGATA, T.; MICHEREFF FILHO, M.; SOUZA, J. O.; RIBEIRO, B. M.; RIBEIRO, S. G.; LACORTE, C.; PEREIRA, J. L.; NAGATA, A. K. I.. |
| Tipo: Parte de livro |
Palavras-chave: Praga; Inseto; Mosca branca; Bemisia Tabaci.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1030982 |
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| ALVES-FREITAS, D. M. T.; PINHEIRO-LIMA, B.; FARIA, J. C.; LACORTE, C.; RIBEIRO, S. G.; MELO, F. L.. |
| Using double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G)... |
| Tipo: Artigo de periódico |
Palavras-chave: Rhabdovirus; Feijão; Phaseolus Vulgaris; Organismo Transgênico; Vírus; Mosaico Dourado; Beans; Cytorhabdovirus; DsRNA viruses; Transgenic plants; Bean golden mosaic virus. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1104582 |
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| ARDISSON-ARAÚJO, D. M. P.; LIMA, R. N.; MELO, F. L.; CLEM, R. J.; HUANG, N.; BÁO, S. N.; SOSA-GÓMEZ, D. R.; RIBEIRO, B. M.. |
| The genome of a novel group II alphabaculovirus, Perigonia lusca single nucleopolyhedrovirus (PeluSNPV), was sequenced and shown to contain 132,831bp with 145 putative ORFs (open reading frames) of at least 50 amino acids. An interesting feature of this novel genome was the presence of a putative nucleotide metabolism enzyme-encoding gene (pelu112). The pelu112 gene was predicted to encode a fusion of thymidylate kinase (tmk) and dUTP diphosphatase (dut). Phylogenetic analysis indicated that baculoviruses have independently acquired tmk and dut several times during their evolution. Two homologs of the tmk-dut fusion gene were separately introduced into the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) genome, which lacks tmk and dut. The... |
| Tipo: Separatas |
Palavras-chave: Baculovirus; Virus; Genoma; Baculoviridae; Genome assembly. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1046809 |
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| Registros recuperados: 30 | |
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