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MENEZES, V. G.; MONTE, A. P. O.; BARBERINO, R. de S.; GOUVEIA, B. B.; LINS, T. L. B.; MACEDO, T. J. S. e; OLIVEIRA JÚNIOR, J. L. de; ARAÚJO, C. de A.; CAMPOS, F. S.; OLIVEIRA FILHO, E. F. de; BATISTA, A. M.; QUEIROZ, M. A. A.; ARAUJO, G. G. L. de; MATOS, M. H. T. de; WISCHRA , A.. |
This study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were... |
Tipo: Artigo de periódico |
Palavras-chave: Foliculogênese; Folículos primordiais; Oócito; Hormônios sexuais; Ovelha; Água Potável; Drinking water; Oocytes; Sex hormones; Sheep. |
Ano: 2023 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156781 |
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MACEDO, T. J. S.; SANTOS, J. M. S.; BEZERRA, M. E. S.; MENEZES, V. G.; GOUVEIA, B. B.; BARBOSA, L. M. R.; LINS, T. L. B. G; MONTE, A. P. O.; BARBERINO, R. S.; BATISTA, A. M.; BARROS, V. R. P.; WISCHRAL, A.; QUEIROZ, M. A. A.; ARAUJO, G. G. L. de; MATOS, M. H. T.. |
The aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM+ alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM+. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM+. In conclusion, leptin and... |
Tipo: Artigo de periódico |
Palavras-chave: Imuno-histoquímica; Desenvolvimento folicular; Imunoexpressão de proteínas receptoras.; Leptina; Maturação de oócitos; Ovino; Ovário. |
Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114397 |
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CAVALCANTI, C. P. L.; MACEDO, T. J. S. e; GOIS, G. C.; MENEZES, V. G.; MONTE, A. P. O. do; SILVA, A. D. da; SILVA, D. J. M. da; SILVA, E. O. da; ARAUJO, G. G. L. de; RODRIGUES, R. T. de S.; WISCHRAL, A.; MATOS, M. H. T. de; QUEIROZ, M. A. A.. |
The aim of this study was to evaluate the effect of inclusion of licuri oil on intake, digestibility, ingestive behavior, rumen protozoa population, and productive performance of Santa In?es ewe. Thirty-two Santa In?es ewe (multiparous, non-lactating, 2?4 years old and 36.7 ± 0.87 kg body weight) were distributed in a randomized block design, receiving diets containing licuri oil (0, 2, 4, and 5% based on total dry matter) in partial replacement of ground corn (n = 8 per treatment). The inclusion of licuri oil promoted a quadratic effect for the intakes of dry matter (P = 0.008), and neutral detergent fiber (P = 0.004), dry matter digestibility (P = 0.004), ether extract (P < 0.0001), average daily gain (P = 0.01), rumination time (min/day; P = 0.039... |
Tipo: Artigo de periódico |
Palavras-chave: Oleo de licuri; Lauric acid; Lipid supplementation; Syagrus coronate; Ácido Laurico; Suplementação lipídica; Ovelhas Santa Inês; Digestibilidade; Ovelha; Nutrição Animal; Ganho de Peso; Dieta; Ewes; Animal nutrition. |
Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154946 |
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LIMA, P. R. de; ARAÚJO, C. de A.; CAMPOS, F. S.; MENEZES, V. G.; RIBEIRO, N. L.; ARAUJO, G. G. L. de; MENEZES, D. R.; MATOS, M. H. T. de; QUEIROZ, M. A. A.; SANTOS, E. M.. |
This study aimed to evaluate the intake and nutrient digestibility, water, and nitrogen balance of Santa In?es crossbred ewes under confinement receiving reductions in the water supply. Thirty-two Santa In?es crossbred ewes, at an average body weight of 32.2 ± 7.4 kg and age of 2.3 ± 0.99 years, were distributed in a completely randomized design, with four treatments, and eight animals per treatment, the treatments were 100%, 80%, 60%, and 40% of ad libitum water. Nutrient intake and digestibility, water and nitrogen balance, and perfor- mance were evaluated. The reductions in the water supply did not promote significant alterations, in the nutrient intake, or apparent digestibility of the animals (P > 0.05). A linear decreasing effect was observed for... |
Tipo: Artigo de periódico |
Palavras-chave: Ingestão de água; Eficiência da água; Fração de nitrogênio; Restrição de água; Semiárido; Raça Santa Inês; Água para ovelhas; Ovelha; Digestibilidade; Produção Animal; Ewes; Animal production. |
Ano: 2023 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154939 |
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