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| Registros recuperados: 13 | |
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| RAMOS, E. Z.; MIDORIKAWA, G. E. O.; FAVARO, L. C. de L.; SILVA, A. S. da; GOTTSCHALK, L. M. F.; BON, E. P. S.. |
| The use of lignocellulosic materials that are the constituents of the plant cell wall have shown to be a great opportunity for sustainable industrial development. The polysaccharide part of these materials, the cellulose and the hemicellulose, can be degraded into their monomeric sugars by microorganisms, such as Trichoderma harzianum, that secrete extracellular enzymes. However, the industrial production of hydrolytic enzymes rely on the development of molecular biology tools to achieve the economical production of enzyme pools with greater hydrolytic potential. In this work the fungus T. harzianum (CFAM-422) was transformed by Agrobacterium tumefaciens and a herbicide was used as a selective agent, making it an efficient strategy to obtain strains with... |
| Tipo: Artigo em anais de congresso (ALICE) |
Palavras-chave: Biologia Molecular; Biologia de Sistemas Aplicadas a Bioprocessos; Trichoderma harzianum. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076996 |
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| MIDORIKAWA, G. E. O.; SOUZA, M. de L. M. de; SILVA, O. F.; DIAS, J. do S. A.; KANZAKI, L. I. B.; HANADA, R. E.; MESQUITA, R. M. L. C.; GONCALVES, R. C.; ALVARES, V. de S.; BITTENCOURT, D. M. de C.; MILLER, R. N. G.. |
| Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Castanha do brasil; Amazônia; Doença de planta; Castanha do pará; Bertholletia excelsa; Fungo; Identificação; Aspergillus flavus; Aflatoxina; Plant diseases and disorders; Brazil nuts; Fungi; Pathogen identification; Enfermedades y desórdenes de las plantas; Nuez del Brasil; Identificación de patógenos. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/987473 |
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| TAMIETTI, M. S.; MIDORIKAWA, G. E. O.; MENDES, T. D.; DAMASO, M. C. T.; BOM, E. P. S.; SILVA, A. S.; GOTTSCHALK, L. M. F.; NORONHA, E. F.; FAVARO, L. C. de L.. |
| Carbon catabolite repression (CCR) is a mechanism by which microorganisms can utilize preferably highly energetic compounds over those of difficult degradation. For Trichoderma reesei, the protein that acts as repressor in the presence of glucose is CRE1. In this project, we aim to delete cre1 gene in Trichoderma harzianum CFAM-422 and obtain mutants with enhanced production of biomass degrading enzymes. Disruption of cre1 in T. harzianum CFAM-422 was performed by gene replacement of cre1 for hph (hygromycin B phosphotransferase) via homologous recombination. Hygromycin resistant mutants and parental strains enzyme production was evaluated in both inductive and repressive conditions in four different carbon sources. Enzymatic indexes (EI) were determined... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Celulase; Processos de hidrolise enzimática de biomassa; Trichoderma harzianum; Carbon Catabolite Repression; Cellulolytic and hemicellulolytic enzymes. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076994 |
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| Registros recuperados: 13 | |
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