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Registros recuperados: 18 | |
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RAMOS, E. Z.; MIDORIKAWA, G. E. O.; FAVARO, L. C. de L.; SILVA, A. S. da; GOTTSCHALK, L. M. F.; BON, E. P. S.. |
The use of lignocellulosic materials that are the constituents of the plant cell wall have shown to be a great opportunity for sustainable industrial development. The polysaccharide part of these materials, the cellulose and the hemicellulose, can be degraded into their monomeric sugars by microorganisms, such as Trichoderma harzianum, that secrete extracellular enzymes. However, the industrial production of hydrolytic enzymes rely on the development of molecular biology tools to achieve the economical production of enzyme pools with greater hydrolytic potential. In this work the fungus T. harzianum (CFAM-422) was transformed by Agrobacterium tumefaciens and a herbicide was used as a selective agent, making it an efficient strategy to obtain strains with... |
Tipo: Separatas |
Palavras-chave: Biologia de Sistemas Aplicadas a Bioprocessos.; Biologia Molecular; Trichoderma Harzianum.. |
Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076996 |
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MIDORIKAWA, G. E. O.; SOUZA, M. de L. M. de; SILVA, O. F.; DIAS, J. do S. A.; KANZAKI, L. I. B.; HANADA, R. E.; MESQUITA, R. M. L. C.; GONCALVES, R. C.; ALVARES, V. de S.; BITTENCOURT, D. M. de C.; MILLER, R. N. G.. |
Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five... |
Tipo: Artigo de periódico |
Palavras-chave: Castanha do brasil; Enfermedades y desórdenes de las plantas; Nuez del Brasil.; Identificación de patógenos; Doença de planta; Castanha do pará; Bertholletia excelsa; Fungo; Identificação; Aspergillus flavus; Aflatoxina; Plant diseases and disorders; Brazil nuts; Fungi; Pathogen identification; Amazonia.. |
Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/987473 |
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TAMIETTI, M. S.; MIDORIKAWA, G. E. O.; MENDES, T. D.; DAMASO, M. C. T.; BOM, E. P. S.; SILVA, A. S.; GOTTSCHALK, L. M. F.; NORONHA, E. F.; FAVARO, L. C. de L.. |
Carbon catabolite repression (CCR) is a mechanism by which microorganisms can utilize preferably highly energetic compounds over those of difficult degradation. For Trichoderma reesei, the protein that acts as repressor in the presence of glucose is CRE1. In this project, we aim to delete cre1 gene in Trichoderma harzianum CFAM-422 and obtain mutants with enhanced production of biomass degrading enzymes. Disruption of cre1 in T. harzianum CFAM-422 was performed by gene replacement of cre1 for hph (hygromycin B phosphotransferase) via homologous recombination. Hygromycin resistant mutants and parental strains enzyme production was evaluated in both inductive and repressive conditions in four different carbon sources. Enzymatic indexes (EI) were determined... |
Tipo: Separatas |
Palavras-chave: Processos de hidrolise enzimática de biomassa; Carbon Catabolite Repression; Cellulolytic and hemicellulolytic enzymes.; Celulase; Trichoderma Harzianum.. |
Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076994 |
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MIDORIKAWA, G. E. O.; SANTOS, L. C. Z.; OLIVEIRA, P. A. de; FAVARO, L. C. de L.. |
Os corantes naturais podem ser extraídos de uma variedade de plantas, animais e microrganismos. A maioria dos corantes naturais utilizados para diferentes fins é produzida a partir de plantas, entretanto, pigmentos extraídos de plantas para uso industrial possuem o gargalo da produção, estabilidade e custo. Uma alternativa mais sustentável para a produção de corantes naturais é a via biotecnológica utilizando microrganismos. O conhecimento de técnicas de cultivo, processamento e facilidade de manipulação, tornam a produção de corantes por microrganismos em larga escala um atrativo para a indústria de cosméticos, de alimentos, têxtil, de pintura, entre outras. Este trabalho apresenta a prospecção de uma coleção de bactérias associadas a plantas nativas e... |
Tipo: Anais e Proceedings de eventos |
Palavras-chave: Metabólicos secundários; Bioprospecção; Corante; Biotecnologia; Microrganismo; Bactéria. |
Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127761 |
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MENDES, T. D.; PACHECO, T. F.; BRAGA, S. C.; NAKAI, D. K.; MIDORIKAWA, G. E. O.; GONCALVES, S. B.; SALUM, T. F. C.; FAVARO, L. C. de L.; DAMASO, M. C. T.. |
Citric acid (CA) is one of the most valuable organic acids in the market as it can be used in diverse biotechnological applications, such as in the food industry, as flavoring agent and as food preservative, besides cosmetics, pharmaceuticals, and cleaning sectors. This acid can be produced by a biotechnological route and the most common producing microorganisms are filamentous fungi and yeasts (Amato, Becci and Beolchini, 2020). Considering that the Brazilian biodiesel production in 2021 was 6.76 million m3, and that 10 kg of crude glycerol are generated for each 100 kg of biodiesel produced, a large amount of that byproduct is available in the market. Then, the objective of this work was to screen filamentous fungi capable of producing citric acid from... |
Tipo: Anais e Proceedings de eventos |
Palavras-chave: Ácido Orgânico; Ácido Cítrico; Food preservatives; Cosmetics; Pharmaceutical industry; Cleaning. |
Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148589 |
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CORRÊA, C. L.; MIDORIKAWA, G. E. O.; FERREIRA FILHO, E. X.; NORONHA, E. F.; ALVES, G. S. C.; TOGAWA, R. C.; SILVA JUNIOR, O. B.; COSTA, M. M. do C.; GRYNBERG, P.; MILLER, R. N. G.. |
Tipo: Artigo de periódico |
Palavras-chave: Lignocellulosic biomass; Biorefinery; Carbohydrate-active enzymes; Aspergillus terreus; Transcriptome. |
Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126336 |
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Registros recuperados: 18 | |
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