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Registros recuperados: 7
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Biodegradability of commercial and weathered diesel oils BJM
Mariano,Adriano Pinto; Bonotto,Daniel Marcos; Angelis,Dejanira de Franceschi de; Pirôllo,Maria Paula Santos; Contiero,Jonas.
This work aimed to evaluate the capability of different microorganisms to degrade commercial diesel oil in comparison to a weathered diesel oil collected from the groundwater at a petrol station. Two microbiological methods were used for the biodegradability assessment: the technique based on the redox indicator 2,6 - dichlorophenol indophenol (DCPIP) and soil respirometric experiments using biometer flasks. In the former we tested the bacterial cultures Staphylococcus hominis, Kocuria palustris, Pseudomonas aeruginosa LBI, Ochrobactrum anthropi and Bacillus cereus, a commercial inoculum, consortia obtained from soil and groundwater contaminated with hydrocarbons and a consortium from an uncontaminated area. In the respirometric experiments it was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradability; Bioremediation; Commercial oil diesel; Weathered diesel oil.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100028
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Comparison of two lipid extraction methods produced by yeast in cheese whey BABT
Castanha,Rodrigo Fernandes; Morais,Lilia Aparecida Salgado de; Mariano,Adriano Pinto; Monteiro,Regina Teresa Rosim.
This work aimed to evaluate nine strains of yeast, previously identified as good producers of lipids in honey medium, for selecting the most suitable strain for the production of lipids in cheese whey medium and compared two well known extraction methods of lipids from the culture medium. The highest yield of total lipids was 1.27 g.L-1 produced by Cryptococcus laurentii 11. A comparison was made between the two culture media: cheese whey and liquid YEPG, and two lipid extraction methods: Bligh and Dyer and Folch et al. for C. laurentii. The experiments were performed with 2² full factorial design using two factors and two levels. Lipid content was higher in cheese whey and there was no difference in the extraction methods statistically. The method of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cheese whey; Yeast; Lipid extraction; Microbial oil.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400013
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Evaluation of the use of vinasse as a biostimulation agent for the biodegradation of oily sludge in soil BABT
Crivelaro,Sérgio Henrique Rezende; Mariano,Adriano Pinto; Furlan,Luis Tadeu; Gonçalves,Renato Almeida; Seabra,Paulo Negrais; Angelis,Dejanira de Franceschi de.
This work aimed to study the effect of vinasse on the biodegradation in soil of oily sludge from the Replan-Petrobras oil refinery. The Bartha respirometric method was used to verify the efficiency of the treatments with soil, oily sludge (7 and 14% m/m) and soil moisture adjustment with or without vinasse (0.11 mL/g dry soil) for 121 days. Although an increase in the soil microbial population was obtained with the vinasse, it demonstrated not to be adequate to enhance the biodegradation efficiency of the oily sludge in soil, since there was no difference between the CO2 produced from treatments with or without vinasse after the complete consumption of the vinasse. Thus, the use of vinasse as an amendment to biodegradation processes showed not to be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Soil; Oily sludge; Vinasse; Landfarming.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500027
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Investigation about the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils BABT
Mariano,Adriano Pinto; Angelis,Dejanira de Franceschi de; Pirôllo,Maria Paula Santos; Contiero,Jonas; Bonotto,Daniel Marcos.
This work investigated the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils collected at three service stations. Batch biodegradation experiments were carried out in Bartha biometer flasks (250 mL) used to measure the microbial CO2 production. Biodegradation efficiency was also measured by quantifying the concentration of hydrocarbons. In addition to the biodegradation experiments, the capability of the studied cultures and the native microorganisms to biodegrade the diesel oil purchased from a local service station, was verified using a technique based on the redox indicator 2,6 -dichlorophenol indophenol (DCPIP). Results obtained with this test showed that the inocula used in the biodegradation experiments were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioremediation; Bioaugmentation; Soil; Diesel oil; Service station.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500030
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Laboratory study on the bioremediation of diesel oil contaminated soil from a petrol station BJM
Mariano,Adriano Pinto; Kataoka,Ana Paula de Arruda Geraldes; Angelis,Dejanira de Franceschi de; Bonotto,Daniel Marcos.
The purpose of the present study was to investigate possible methods to enhance the rate of aerobic biodegradation of hydrocarbons (ex-situ treatments). In this work, the bioremediation processes were applied to a sandy soil with a high level of contamination originated from the leakage of a diesel oil underground storage tank at a petrol station. Laboratory scale experiments (Bartha biometer flasks) were used to evaluate the biodegradation of the diesel oil. Enhancement of biodegradation was carried out through biostimulation (addition of nitrogen and phosphorus solutions or Tween 80 surfactant) and bioaugmentation (bacterial consortium isolated from a landfarming system). To investigate interactions between optimizing factors, and to find the right...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioremediation; Soil; Diesel Oil; Petrol Station.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200030
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Optimization of lipids production by Cryptococcus laurentii 11 using cheese whey with molasses BJM
Castanha,Rodrigo Fernandes; Mariano,Adriano Pinto; Morais,Lilia Aparecida Salgado de; Scramin,Shirlei; Monteiro,Regina Teresa Rosim.
This study aimed the optimization of culture condition and composition for production of Cryptococcus laurentii 11 biomass and lipids in cheese whey medium supplemented with sugarcane molasses. The optimization of pH, fermentation time, and molasses concentration according to a full factorial statistical experimental design was followed by a Plackett-Burman experimental design, which was used to determine whether the supplementation of the culture medium by yeast extract and inorganic salts could provide a further enhancement of lipids production. The following conditions and composition of the culture medium were found to optimize biomass and lipids production: 360 h fermentation, 6.5 pH and supplementation of (g L-1): 50 molasses, 0.5 yeast extract, 4...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryptococcus laurentii; Microbial lipids; Cheese whey; Sugarcane molasses; Biodiesel.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200003
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The use of vinasse as an amendment to ex-situ bioremediation of soil and groundwater contaminated with diesel oil BABT
Mariano,Adriano Pinto; Crivelaro,Sérgio Henrique Rezende; Angelis,Dejanira de Franceschi de; Bonotto,Daniel Marcos.
This work investigated the possibility of using vinasse as an amendment in ex-situ bioremediation processes. Groundwater and soil samples were collected at petrol stations. The soil bioremediation was simulated in Bartha biometer flasks, used to measure the microbial CO2 production, during 48 days, where vinasse was added at a concentration of 33 mL.Kg-1of soil. Biodegradation efficiency was also measured by quantifying the total petroleum hydrocarbons (TPH) by gas chromatography. The groundwater bioremediation was carried out in laboratory experiments simulating aerated (bioreactors) and not aerated (BOD flasks) conditions. In both the cases, the concentration of vinasse was 5 % (v/v) and different physicochemical parameters were evaluated during 20 days....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioremediation; Soil; Groundwater; Diesel oil; Biostimulation; Vinasse.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400030
Registros recuperados: 7
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