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Duplex nested-PCR for detection of small ruminant lentiviruses 58
Marinho,Rebeca C.; Martins,Gabrielle R.; Souza,Kelma C.; Sousa,Ana Lídia M.; Silva,Sabrina Tainah C.; Nobre,Juliana A.; Teixeira,Maria F.S..
Abstract Small ruminant lentiviruses (SRLV) have high genetic variability which results in different viral strains around the world. This create a challenge to design sensible primers for molecular diagnosis in different regions. This work proposes a protocol of duplex nested-PCR for the precise diagnosis of SRLV. The technique was designed and tested with the control strains CAEV Co and MVV 1514. Then, field strains were submitted to the same protocol of duplex nested-PCR. Blood samples of sheep and goats were tested with AGID and nested PCR with specific primers for pol, gag and LTR. The AGID results showed low detection capacity of positive animals, while the nested PCR demonstrated a greater capacity of virus detection. Results demonstrated that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Standardization; Multiplex; Viruses; CAEV; MVV.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500083
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Goat umbilical cord cells are permissive to small ruminant lentivirus infection in vitro 58
Martins,Gabrielle R.; Marinho,Rebeca C.; Bezerra Junior,Rosivaldo Q.; Alves,Antoniel de O.; Câmara,Lilia M.C.; Albuquerque-Pinto,Luiz C.; Teixeira,Maria F. da S..
Abstract Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture. To accomplish this goal, cells were isolated from umbilical cords; characterized phenotypically by flow cytometry analysis; differentiate into osteogenic,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CAEV; MVV; Cell culture; MSC; Flow cytometry.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100125
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