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Registros recuperados: 49 | |
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Le Boucher, Richard; Vandeputte, Marc; Dupont-nivet, Mathilde; Quillet, Edwige; Mazurais, David; Robin, Jean; Vergnet, Alain; Medale, Francoise; Kaushik, Sadasivam; Chatain, Beatrice. |
This preliminary study assessed genotype x diet interaction in late growth of European sea bass (Dicentrarchus labrax) fed with either a fish meal (FM)- or a fish oil (FO)-based diet (M) or an all-plant-based (PB) diet. A total of 550 fish from 224 families were reared together and tagged. DNA was sampled and microsatellites were used to assign parentage. When fish weight was 192 +/- 54 g, two tanks were fed with M (FM: 100%; FO: 100%) and two others with PB (FM: 0% and FO: 0%). Body weight (BW), fork length (FL) and fillet lipid content (CorrFat) were analysed with a linear model and with REML methodology. We observed no significant differences between groups, but a slightly lower (P=0.03) daily growth coefficient in sea bass fed PB than in those fed M.... |
Tipo: Text |
Palavras-chave: Genotype by diet x environment interaction; European sea bass; Plant diet; Genetic correlation. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00032/14335/12374.pdf |
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Le Boucher, Richard; Vandeputte, Marc; Dupont Nivet, Mathilde; Quillet, Edwige; Mazurais, David; Robin, Jean; Vergnet, Alain; Medale, Francoise; Kaushik, Sadasivam; Chatain, Beatrice. |
Tipo: Text |
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Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00038/14907/12233.pdf |
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Zambonino, Jose-luis; Mazurais, David; Dubuc, Alexia; Queau, Pierre; Vanderplancke, Gwenaelle; Servili, Arianna; Cahu, Chantal; Le Bayon, Nicolas; Huelvan, Christine; Claireaux, Guy. |
Ocean warming, eutrophication and consequent decrease in oxygen lead to smaller average fish size. Although such responses are well-known in an evolutionary context, involving multiple generations, it appears to be incompatible with current rapid environmental change. Rather, phenotypic plasticity could provide a means for marine fish to cope with rapid environmental changes. However, little is known about the mechanisms underlying plastic responses to environmental conditions that favour small phenotypes. Our aim was to investigate how and why European sea bass that had experienced a short episode of moderate hypoxia during their larval stage subsequently exhibited a growth depression at the juvenile stage compared to the control group. We examined... |
Tipo: Text |
Palavras-chave: Climate change; Digestive enzymes; Dicentrarchus labrax; Growth; Hypoxia; Respirometry. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00373/48407/48635.pdf |
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Destoumieux-garzón, Delphine; Bonnet, P.; Teplitsky, C.; Criscuolo, F.; Henry, P.-y.; Mazurais, David; Prunet, P.; Salvat, Gilles; Usseglio-polatera, P.; Verrier, E.; Friggens, N.c.. |
Wild and farmed animals are key elements of natural and managed ecosystems that deliver functions such as pollination, pest control and nutrient cycling within the broader roles they play in contributing to biodiversity and to every category of ecosystem services. They are subjected to global changes with a profound impact on the natural range and viability of animal species, the emergence and spatial distribution of pathogens, land use, ecosystem services and farming sustainability. We urgently need to improve our understanding of how animal populations can respond adaptively and therefore sustainably to these new selective pressures. In this context, we explored the common points between animal production science and animal ecology to identify promising... |
Tipo: Text |
Palavras-chave: Adaptation; Agroecosystem; Livestock sustainability; Resilience; Zoonotic disease. |
Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00663/77509/79273.pdf |
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Vignet, Caroline; Le Menach, Karyn; Mazurais, David; Lucas, Julie; Perrichon, Prescilla; Le Bihanic, Florane; Devier, Marie-hélène; Lyphout, Laura; Frere, Laura; Begout, Marie-laure; Zambonino-infante, Jose Luis; Budzinski, Helene; Cousin, Xavier. |
The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades leading to high concentrations in sediments of contaminated areas. To evaluate the consequences of long-term chronic exposure to PAHs, zebrafish were exposed, from their first meal at 5 days post fertilisation until they became reproducing adults, to diets spiked with three PAH fractions at three environmentally relevant concentrations with the medium concentration being in the range of 4.6–6.7 μg g−1 for total quantified PAHs including the 16 US-EPA indicator PAHs and alkylated derivatives. The fractions used were representative of PAHs of pyrolytic (PY) origin or of two different oils of differing compositions, a heavy fuel... |
Tipo: Text |
Palavras-chave: Danio rerio; Life cycle; Polycyclic aromatic hydrocarbon; Heavy oil; Light crude oil; Alkylated PAH; Jaw; Digestion. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29907/28490.pdf |
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Geay, Florian; Darias, Maria J.; Santigosa, E.; Desbruyeres, Elisabeth; Quazuguel, Patrick; Zambonino, Jose-luis; Cahu, Chantal; Mazurais, David. |
It is known in vertebrates that endothelin-1 (ET-1) plays a key role ill morphogenesis whose expression level greatly influences the development of craniofacial malformations. In the Present study, the complete cDNA fragment encoding a precursor of endothelin-1, the preproendothelin-1 (PPET1), was cloned by RACE-PCR from European sea bass. The cDNA encoded a 199 amino acid polypeptide that was composed of the "mature" and "big" ET-1. Relative ET-1 expression levels were investigated in European sea bass larvae fed microparticulate diets containing the standard amount (0.08 g retinol/kg diet: group N) or all excess of retinoic acid which induces skeletal malformations (0.5 g retinol/kg diet: group RA). Real-time reverse transcriptor polymerase chain... |
Tipo: Text |
Palavras-chave: European sea bass; Retinoic acid; Morphogenesis; Gene expression; Endothelin 1. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6172.pdf |
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Santigosa, Ester; Geay, Florian; Tonon, Thierry; Le Delliou, Herve; Kuhl, Heiner; Reinhardt, Richard; Corcos, Laurent; Cahu, Chantal; Zambonino, Jose-luis; Mazurais, David. |
Fish are the main source of the n-3 highly unsaturated fatty acids, which are crucial for human health. Their synthesis from C18 precursors is mediated by desaturases and elongases, but the activity of these enzymes has not been conclusively established in marine fish species. This study reports the cloning, tissue expression, and functional characterization of a sea bass (Dicentrarchus labrax L.) Δ6-desaturase and one of its splicing variants. Two cDNAs with open reading frames of 1,346 and 1,354 bp were cloned and named D6D and D6D-V, respectively. Both deduced protein sequences (445 and 387 amino acids, respectively) contained two transmembrane regions and the N-terminal cytochrome b5 domain with the HPGG motif characteristic of microsomal desaturases.... |
Tipo: Text |
Palavras-chave: Sea bass (Dicentrarchus labrax); Desaturase; HUFA biosynthesis; Fish; EPA. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00023/13470/10477.pdf |
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Lamari, Faouzi; Castex, Mathieu; Larcher, Thibaut; Ledevin, Mireille; Mazurais, David; Bakhrouf, Amina; Gatesoupe, Joel. |
Probiotics may have many effects on health and development of fish larvae. One of the most promising is related to spinal conformation, though the mode of action is not clearly understood. The present study attempted to investigate the effects of two strains of lactic acid bacteria on associated microbiota, histological development and gene expression. Sea bass larvae were fed since 5 dph (day post hatch) with either a standard control diet (Diet C), or the same diet supplemented with Pediococcus acidilactici MA18/5M (Diet P), or with an autochthonous strain of Lactobacillus casei (X2; Diet L). The two lactic acid bacteria were incorporated in the diets at the levels of 106 and 107 CFU (colony forming units) g-1 in two consecutive experiments,... |
Tipo: Text |
Palavras-chave: Fish larvae; Probiotics; Microbiota; Histopathology; Gene expression; DGGE. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00108/21966/19673.pdf |
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Petitgas, Pierre; Ulrich, Clara; Auber, Arnaud; Gourguet, Sophie; Huret, Martin; Mazurais, David; Pernet, Fabrice; Pouvreau, Stephane; Richard, Marion; Servili, Arianna; Thebaud, Olivier; Zambonino Infante, Jose-luis. |
Tipo: Text |
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Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00657/76942/78148.pdf |
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Paul-pont, Ika; Tallec, Kevin; Gonzalez-fernandez, Carmen; Lambert, Christophe; Vincent, Dorothee; Mazurais, David; Zambonino-infante, Jose-luis; Brotons, Guillaume; Lagarde, Fabienne; Fabioux, Caroline; Soudant, Philippe; Huvet, Arnaud. |
Marine plastic pollution is a major environmental issue. Given their ubiquitous nature and small dimensions, ingestion of microplastic (MP) and nanoplastic (NP) particles and their subsequent impact on marine life are a growing concern worldwide. Transfers along the trophic chain, including possible translocation, for which the hazards are less understood, are also a major preoccupation. Effects of MP ingestion have been studied on animals through laboratory exposure, showing impacts on feeding activity, reserve depletion and inflammatory responses, with consequences for fitness, notably reproduction. However, most experimental studies have used doses of manufactured virgin microspheres that may not be environmentally realistic. As for most... |
Tipo: Text |
Palavras-chave: Microplastics; Nanoplastics; Experimental exposure; Impacts; Marine organisms; Physiology. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00449/56088/57621.pdf |
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Fernandez, Ignacio; Darias, Maria; Andree, Karl B.; Mazurais, David; Zambonino, Jose-luis; Gisbert, Enric. |
Background: Vitamin A (VA) has a key role in vertebrate morphogenesis, determining body patterning and growth through the control of cell proliferation and differentiation processes. VA regulates primary molecular pathways of those processes by the binding of its active metabolite (retinoic acid) to two types of specific nuclear receptors: retinoic acid receptors (RARs) and retinoid X receptors (RXRs), which promote transcription of downstream target genes. This process is well known in most of higher vertebrates; however, scarce information is available regarding fishes. Therefore, in order to gain further knowledge of fish larval development and its disruption by nutritional VA imbalance, the relative expression of some RARs and RXRs, as well as several... |
Tipo: Text |
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Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00031/14264/11546.pdf |
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Benzekri, Hicham; Armesto, Paula; Cousin, Xavier; Rovira, Mireia; Crespo, Diego; Alejandro Merlo, Manuel; Mazurais, David; Bautista, Rocio; Guerrero-fernandez, Dario; Fernandez-pozo, Noe; Ponce, Marian; Infante, Carlos; Zambonino, Jose Luis; Nidelet, Sabine; Gut, Marta; Rebordinos, Laureana; Planas, Josep V.; Begout, Marie-laure; Gonzalo Claros, M.; Manchado, Manuel. |
Background Senegalese sole (Solea senegalensis) and common sole (S. solea) are two economically and evolutionary important flatfish species both in fisheries and aquaculture. Although some genomic resources and tools were recently described in these species, further sequencing efforts are required to establish a complete transcriptome, and to identify new molecular markers. Moreover, the comparative analysis of transcriptomes will be useful to understand flatfish evolution. Results A comprehensive characterization of the transcriptome for each species was carried out using a large set of Illumina data (more than 1,800 millions reads for each sole species) and 454 reads (more than 5 millions reads only in S. senegalensis), providing coverages ranging from... |
Tipo: Text |
Palavras-chave: Soles; Transcriptome; Microarray; Orthology; Molecular markers; SoleaDB. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00238/34918/33626.pdf |
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Vagner, Marie; Lacoue-labarthe, Thomas; Infante, Jose-luis Zambonino; Mazurais, David; Dubillot, Emmanuel; Le Delliou, Herve; Quazuguel, Patrick; Lefrancois, Christel. |
The objective of this study was to evaluate the combined effects of thermal acclimation and n-3 highly unsaturated fatty acids (n-3 HUFA) content of the food source on the aerobic capacities of fish in a thermal changing environment. The model used was the golden grey mullet Liza aurata, a species of high ecological importance in temperate coastal areas. For four months, fish were exposed to two food sources with contrasting n-3 HUFA contents (4.8% ecosapentaenoic acid EPA + docosahexaenoic acid DHA on the dry matter DM basis vs. 0.2% EPA+DHA on DM) combined with two acclimation temperatures (12 degrees C vs. 20 degrees C). The four experimental conditions were LH12, LH20, HH12 and HH20. Each group was then submitted to a thermal challenge consisting of... |
Tipo: Text |
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Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00274/38479/36939.pdf |
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Callet, Therese; Dupont-nivet, Mathilde; Cluzeaud, Marianne; Jaffrezic, Florence; Laloe, Denis; Kerneis, Thierry; Labbe, Laurent; Quillet, Edwige; Geurden, Inge; Mazurais, David; Skiba-cassy, Sandrine; Medale, Francoise. |
o meet the growing demand of fish feed for aquaculture, an increasing proportion of marine ingredients are being replaced by blends of plant products. However, the total replacement of marine ingredients in salmonid diets impairs fish performance. This is particularly true during the early fry stage and this stage is therefore considered of particular importance. In rainbow trout (RBT), the existence of a genetic variability to survive and grow with plant-based diets devoid of marine ingredients has now been proved, but the mechanisms behind are little studied especially at early stage. To investigate these, we analysed the whole transcriptome of three isogenic lines of RBT fry, which have similar growth when fed a marine resources-based diet (M diet) but... |
Tipo: Text |
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Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00452/56371/57969.pdf |
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Tovar-ramirez, D.; Mazurais, David; Gatesoupe, J. F.; Quazuguel, Patrick; Cahu, Chantal; Zambonino-infante, J. L.. |
The main goal of this work was to determine the effect of dietary live yeast Debaryomyces hansenii on the enzymatic antioxidative status of sea bass Dicentrarchus labrax larvae. Growth, activity and expression of the main antioxidative enzymes: catalase (CAT), glutathione peroxidase (GPX) and superoxide dismutase (SOD), and heat shock protein (HSP70) were measured in sea bass larvae at 23 and 48 days after hatching. Larvae were fed on two microdiets: group one, fed microdiet containing live yeast and the control group fed microdiet without yeast. Heat shock protein 70 showed the same expression levels in both fish larvae fed yeast and the control diet. The group fed D. hansenii showed highest growth and lower activity and expression levels of GPX and SOD... |
Tipo: Text |
Palavras-chave: Antioxidant enzymes; Probiotics; Debaryomyces hansenii; Dicentrarchus labrax. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11299/7881.pdf |
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Darias, Maria J.; Mazurais, David; Koumoundouros, Giorgos; Glynatsi, Nomiki; Christodoulopoulou, Stavroula; Huelvan, Christine; Desbruyeres, Elisabeth; Le Gall, Marie-madeleine; Quazuguel, Patrick; Cahu, Chantal; Zambonino-infante, Jose L.. |
The influence of dietary vitamin D-3 (VD3) on survival, growth and morphogenesis during the larval development of European sea bass (Dicentrarchus labrax) was evaluated until 45 days post hatching. Diets contained 4% of the standard vitamin mix (VM) recommended by the National Research Council (NRC) and incorporated 0, 19.2, 38.4, or 140 IU of VD3 per gram of diet to give VD-0, VD-1, VD-2 and VD-3 dietary treatments, respectively. The present study revealed for the first time an impact of dietary VD3 on the sea bass digestive system ontogenesis that consequently conditioned the ossification process and morphogenesis. All dietary VD3 levels were in the "adequate range" based on larval survival. Nevertheless, growth, intestinal maturation and ossification at... |
Tipo: Text |
Palavras-chave: Fish larvae; Ossification; Malformations; Vitamin D; Digestive system; Gene expression. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00000/11147/7849.pdf |
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Darias, M. J.; Wing, O. Lan Chow; Cahu, Chantal; Zambonino, Jose-luis; Mazurais, David. |
P>The alcian blue-alizarin red technique was successfully adjusted to stain developing European sea bass (Dicentrarchus labrax) larvae. For an optimal staining protocol design both larval size and their morphological characteristics at each developmental stage were considered, since such parameters notably influence the staining of tissues. The incubation times of the different solutions were adjusted to allow the stain penetration for revealing the integrity of cartilaginous and bony tissues without significant tissue degradation. Three developmental windows were determined for an optimal staining procedure: (i) 4.5-6.4 mm, (ii) 6.7-8.7 mm, and (iii) 12.8-15.5 mm total length (TL). In order to validate the continuity of staining along the larval... |
Tipo: Text |
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Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11371/8364.pdf |
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Registros recuperados: 49 | |
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