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Registros recuperados: 8
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Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants Scientia Agricola
Ditt,Renata Fava; Gentile,Agustina; Tavares,Rafael Garcia; Camargo,Sandra Rodriguez; Fernandez,Jorge Hernandez; Silva,Marcio Jose da; Menossi,Marcelo.
Stresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp.) is cultivated in tropical and subtropical areas and is considered a cold-sensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of those genes, SsNAC23, a member of the NAC family of plant-specific transcription factors, which are induced by low temperature and other stresses in several plant species. The expression of SsNAC23 was induced in sugarcane plants exposed to low temperatures (4ºC). With the aim of further understanding the regulatory network in response to stress, we used the yeast two-hybrid system to identify...
Tipo: Info:eu-repo/semantics/article Palavras-chave: SsNAC; In situ hybridization cold stress yeast two hybrid.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400010
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Bringing to light the molecular evolution of GUX genes in plants Genet. Mol. Biol.
Gallinari,Rafael Henrique; Coletta,Rafael Della; Araújo,Pedro; Menossi,Marcelo; Nery,Mariana Freitas.
Abstract Hemicellulose and cellulose are essential polysaccharides for plant development and major components of cell wall. They are also an important energy source for the production of ethanol from plant biomass, but their conversion to fermentable sugars is hindered by the complex structure of cell walls. The glucuronic acid substitution of xylan (GUX) enzymes attach glucuronic acid to xylan, a major component of hemicellulose, decreasing the efficiency of enzymes used for ethanol production. Since loss-of-function gux mutants of Arabidopsis thaliana enhance enzyme accessibility and cell wall digestion without adverse phenotypes, GUX genes are potential targets for genetically improving energy crops. However, comprehensive identification of GUX in...
Tipo: Info:eu-repo/semantics/other Palavras-chave: GUX; Sugarcane; Phylogeny; Angiosperms; Biofuels.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100602
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Evaluation of the performance of different plastics used to seal nylon cDNA arrays Ciência e Agrotecnologia
Costa Netto,Antônio Paulino da; Drummond,Rodrigo Duarte; Felix,Juliana de Maria; Jorge,Renato Atílio; Menossi,Marcelo.
cDNA arrays are a powerful tool for discovering gene expression patterns. Nylon arrays have the advantage that they can be re-used several times. A key issue in high throughput gene expression analysis is sensitivity. In the case of nylon arrays, signal detection can be affected by the plastic bags used to keep membranes humid. In this study, we evaluated the effect of five types of plastics on the radioactive transmittance, number of genes with a signal above the background, and data variability. A polyethylene plastic bag 69 μm thick had a strong shielding effect that blocked 68.7% of the radioactive signal. The shielding effect on transmittance decreased the number of detected genes and increased the data variability. Other plastics which were thinner...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Signal detection; Nylon arrays; Sensitivity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000700031
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Identification of methyl jasmonate-responsive genes in sugarcane using cDNA arrays Braz. J. Plant Physiol.
De Rosa Jr.,Vicente E.; Nogueira,Fábio T. S.; Menossi,Marcelo; Ulian,Eugênio C.; Arruda,Paulo.
Jasmonic acid (JA) and its ester methyl jasmonate (MeJA) are linolenic acid-derived signaling molecules involved in plant development and stress responses. MeJA regulates gene expression at transcription, RNA processing and translation. We investigated the changes in gene expression in sugarcane leaves exposed to MeJA using cDNA arrays. Total RNA isolated at 0, 0.5, 1, 3, 6, and 12 h following MeJA treatment was labeled with alpha-33P-dCTP and hybridized to nylon filters containing 1,536 cDNA clones. A significant increase in gene expression in response to MeJA was detected for both novel and well known stress-related genes, while genes participating in photosynthesis and carbohydrate assimilation were down-regulated. Searches for conserved domains in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CDNA array; Expression profiling; Methyl-jasmonate; Signaling molecules; Sugarcane.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100014
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In silico evaluation of the Eucalyptus transcriptome Genet. Mol. Biol.
Vicentini,Renato; Sassaki,Flávio T.; Gimenes,Marcos A.; Maia,Ivan G.; Menossi,Marcelo.
The expressed sequence tags (ESTs) produced in the Forests project provide an invaluable opportunity to assess the Eucalyptus transcriptome. Besides providing information on the different proteins produced by this plant, it is possible to infer gene expression profiles because non-normalized cDNA libraries were used. The EST frequency from any gene is correlated to the transcript levels in the tissues from which the cDNA libraries were constructed. The goal of this work was to identify Eucalyptus genes that showed either differential expression pattern or were ubiquitously expressed in the tissues sampled in the Forests project. Six robust statistical tests and very restrictive rules were applied to gain confidence in the in silico data aiming to avoid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transcriptome; Eucalyptus; Tissue-specific; Statistics; Differential expression.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400002
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Metabolism and root exudation of organic acid anions under aluminium stress Braz. J. Plant Physiol.
Mariano,Eduardo D.; Jorge,Renato A.; Keltjens,Willem G.; Menossi,Marcelo.
Numerous plant species can release organic acid anions (OA) from their roots in response to toxic aluminium (Al) ions present in the rooting medium. Hypothetically OA complex Al in the root apoplast and/or rhizosphere and thus avoid its interaction with root cellular components and its entry in the root symplast. Two temporal patterns of root OA exudation are observed. In pattern I, OA release is rapidly activated after the contact of the root with Al ions while in pattern II there is a lag phase between the addition of Al and the beginning of OA release. Compounds other than OA have been detected in root exudates and are also correlated with Al resistance in plants. Plant species like buckwheat and tea show mechanisms of Al tolerance, which confer them...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Al resistance; Al toxicity; Anion channels; Root exudates; Transgenic plants.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100013
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pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops Genet. Mol. Biol.
Guidelli,Giovanna V.; Mattiello,Lucia; Gallinari,Rafael H.; Lucca,Paulo Cezar de; Menossi,Marcelo.
Abstract The successful development of genetically engineered monocots using Agrobacterium-mediated transformation has created an increasing demand for compatible vectors. We have developed a new expression vector, pGVG, for efficient transformation and expression of different constructs for gene overexpression and silencing in sugarcane. The pCAMBIA2300 binary vector was modified by adding Gateway recombination sites for fast gene transfer between vectors and the maize polyubiquitin promoter Ubi-1 (ZmUbi1), which is known to drive high gene expression levels in monocots. Transformation efficiency using the pGVG vector reached up to 14 transgenic events per gram of transformed callus. Transgenic plants expressing the β-glucuronidase (GUS) reporter gene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monocots; Sugarcane; Vector; Gateway technology; Genetic transformation.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300450
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Prospecting sugarcane genes involved in aluminum tolerance Genet. Mol. Biol.
Drummond,Rodrigo D.; Guimarães,Claudia T.; Felix,Juliana; Ninamango-Cárdenas,Fernando E.; Carneiro,Newton P.; Paiva,Edilson; Menossi,Marcelo.
Aluminum is one of the major factors that affect plant development in acid soils, causing a substantial reduction in yield in many crops. In South America, about 66% of the land surface is made up of acid soils where high aluminum saturation is one of the main limiting factors for agriculture. The biochemical and molecular basis of aluminum tolerance in plants is far from being completely understood despite a growing number of studies, and in the specific case of sugarcane there are virtually no reports on the effects of gene regulation on aluminum stress. The objective of the work presented in this paper was to prospect the sugarcane expressed sequence tag (SUCEST) data bank for sugarcane genes related to several biochemical pathways known to be involved...
Tipo: Info:eu-repo/semantics/article
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100029
Registros recuperados: 8
Primeira ... 1 ... Última
 

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