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Immunocytochemical and biochemical detection of alpha-L-fucosidase in Trypanosoma cruzi 56
Miletti,L.C.; Almeida-de-Faria,M.; Colli,W.; Alves,M.J.M..
The aim of the present study was to demonstrate the presence of alpha-L-fucosidase in Trypanosoma cruzi. Immunocytochemical and biochemical techniques were used to localize and characterize a membrane-associated, neutral-pH-optimum, alpha-L-fucosidase from the parasite. Light and electron microscopy localized the alpha-L-fucosidase specifically on the surface of the parasite and on membranes in the posterior region of the epimastigote stage. Although much less intense, labeling was also detected on the surface of trypomastigotes. At least 50% of the alpha-L-fucosidase activity was associated with epimastigote membrane solubilized with 1 M NaCl or 1% Triton X-100, suggesting that alpha-L-fucosidase is peripherally associated with membranes. The enzyme from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosoma cruzi; Trypanosomatids; Fucosidase; Fucose; Glycoproteins; Glycolipids.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500006
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Toxoplasma gondii in milk of naturally infected dairy ewes on west mesoregion of Santa Catarina state, Brazil 45
Ossani,R.A.; Borges,H.A.T.; Souza,A.P.; Sartor,A.A.; Miletti,L.C.; Federle,M.; Moura,A.B..
ABSTRACT This study aimed to detect Toxoplasma gondii in the milk of dairy sheep in the Western mesorregion of state of Santa Catarina by bioassay (22 milk samples from eight ewes seropositive; IFA ≥256) and PCR [for the detection of agent in the brains of mice inoculated on bioassay and directly from milk (108 samples from 42 seropositive ewes (IFA, ≥64) in different lactation periods)]. T. gondii DNA was detected in mice brains inoculated with milk from eight sheep (a sample of the 45th day of lactation and seven in the collection of 90th day) and directly from the milk in samples of the second collection (90 days) in five animals. Taking into account both assays, from a total of 42 ewes in lactation and seropositive for T. gondii, 30.95% (13/42) of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxoplasma gondii; Milk sheep; PCR; Santa Catarina state.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501294
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