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| Registros recuperados: 29 | |
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| Fleury, Pierre-gildas; Mingant, Christian; Castillo, A. |
| In order to have a better understanding of recessing in great scallop, Pecten maximus and consequently the causes of mortality at reseeding, this study has monitored, at different seasons, the dispersion and recessing of different sizes of juveniles (about 15, 30 and 45 mm, called small, medium and large) after seeding. Moreover, the aim was to see when small spat (15 mm) could be seeded, and thus reduce the costs of intermediate culture. Three monitoring approaches were used together: (1) continual observations by remote video camera, of a defined area (less than 1 m2) containing 10 scallops from each size group; (2) daily monitoring of behaviour with divers along three bottom lines, with 20 × 1 m2 plots each and nine marked scallops per plot; and (3) the... |
| Tipo: Text |
Palavras-chave: Behaviour; Great scallop (Pecten maximus); Season; Seeding; Size; Vitality of spat. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00326/43752/43284.pdf |
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| Belsher, Thomas; Lunven, Michel; Le Gall, Erwan; Caisey, Xavier; Dugornay, Olivier; Mingant, Christian. |
| The oceanographic cruise Califa 2000, as the previous cruise, which concerned the "Parc National de Port-Cros", had for its principal objective to furnish some elements susceptible to help in the control of Caulerpa taxifolia expansion and in the protection of particularly sensitive areas (national parks, patrimonial sectors, regions of socio-economic importance). The cruise lasted from the 4th to the 21st of September 2000 in the rade d'Hyeres, Porquerolles island included, and the rade of Toulon. This detailed prospection gives a location of C. taxifolia and C. racemosa, a new invasive seaweed. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved. |
| Tipo: Text |
Palavras-chave: Rade de Toulon; Rade d' Hyeres; Cartography; Caulerpa racemos; Caulerpa taxifolia; Invasive species; Rade de Toulon; Rade d'Hyères; Cartographie; Caulerpa racemosa; Caulerpa taxifolia; Espèces invasives. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-520.pdf |
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| Prudence, Marie; Moal, Jeanne; Boudry, Pierre; Daniel, Jean-yves; Quere, Claudie; Jeffroy, Fanny; Mingant, Christian; Ropert, Michel; Bedier, Edouard; Van Wormhoudt, A; Samain, Jean-francois; Huvet, Arnaud. |
| This study investigated the non-neutrality of genetic polymorphism in two alpha-amylase genes (AMYA and AMYB) in the oyster Crassostrea gigas. Bi-parental oyster families, bred to be polymorphic for markers in these genes, were monitored for growth and survival for 1 year under standard culture conditions in two French production sites. Within-family genotype frequencies indicated that the two amylase genes were closely linked (c. 1.7 cM). Within two of three families, significant differences in growth were observed between genotypes at one of the two production sites, suggesting that this polymorphism is not neutral and might be under selection because of its role in digestive function. Estimated daily yields were different between amylase genotypes,... |
| Tipo: Text |
Palavras-chave: Growth; Genotype; Digestive enzyme; Bivalve; Alpha amylase. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1691.pdf |
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| Suquet, Marc; Gonzalez, Ricardo; Lebrun, Luc; Queau, Isabelle; Mingant, Christian; Robert, Rene. |
| Controlled reproduction of the European flat oyster requires the development of tools adapted to this species, including a practical anaesthesia and gonad sampling protocol to facilitate sex determination and the verification of gametogenesis. Three replicate groups of 10 oysters (mean weight +/- SD: 29.9 +/- 8.5 g) were anaesthetised in 5 L containers using magnesium chloride designed either for laboratory (Flucka (R)) or agricultural (Dead Sea Work (R): DSW) use, at two concentrations (50 or 72 g L-1). No significant differences were observed in the percentages of oysters anaesthetised or subsequent oyster mortality with the different anaesthetics or concentrations, but increasing water temperature from 14.9 to 18.8 degrees C significantly increased the... |
| Tipo: Text |
Palavras-chave: Oyster; Ostrea edulis; Anaesthesia; Magnesium chloride; Gonad sampling. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00018/12907/9876.pdf |
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| Suquet, Marc; De Kermoysan, Goulwen; Araya, Ricardo Gonzalez; Queau, Isabelle; Lebrun, Luc; Le Souchu, Pierrick; Mingant, Christian. |
| A reliable anaesthesia and sampling protocol for Pacific oysters will enable experiments to be conducted without sacrificing animals and will facilitate successive sampling of individuals for gametogenesis studies. As no such techniques were available for Crassostrea gigas, the present study aimed to define suitable anaesthetic conditions for use with this species. Three groups of ten oysters (mean weight +/- SD, 32.1 +/- 9.0 g) were anaesthetised in 5 L containers. Among different chemicals: benzocaine, eugenol and three different types of magnesium chloride (a laboratory one Flucka (R) - and two designed for agriculture - DEUSA International (R) and Dead SeaWorks (R)) and concentrations tested, one laboratory (concentration: 72 g L-1) and one... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Pacific oyster; Gonad sampling; Magnesium chloride; Relaxant; Anaesthesia. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6352.pdf |
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| Labbe, Catherine; Haffray, Pierrick; Mingant, Christian; Quittet, Benjamin; Diss, Blandine; Tervit, H. Robin; Adams, Serean L.; Rimond, Flore; Suquet, Marc. |
| Pacific oyster Crassostrea gigas is one major species for aquaculture, and the development of breeding programs and the need for preservation of wild stock genetic resources prompted the need for larvae cryopreservation. The objective of the present study was to choose the most reliable protocol from several existing publications, to test its biological and practical limitations, and to adapt it to hatchery conditions. The selected protocol was characterized by a very slow freezing rate without seeding, and by the use of ethylene glycol and sucrose as cryoprotectant. The best survivals after thawing and rearing up to 48 h post fertilization (hpf) were obtained with larvae that were frozen at late trochophore (20 hpf) and early-D (24 hpf) stages. Increasing... |
| Tipo: Text |
Palavras-chave: Oyster; Cryobanking; D stage; Ethylene glycol; High throughput; Hatchery. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00423/53460/54354.pdf |
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| Suquet, Marc; Rimond, Flore; Cosson, J.; Wilson-leedy, J.; Lebrun, Luc; Queau, Isabelle; Mingant, Christian; Fauvel, Christian. |
| The movement characteristics of Pacific oyster trochophores have received very little coverage in the scientific literature. Described here are not only changes in the swimming characteristics of Crassostrea gigas trochophores (size: 53-77 mu m) in relation to time after fertilization and to inter-female variation but also the effects of salinity and pH on movement characteristics. The percentage of motile trochophores was measured on images obtained through a dissecting microscope and the Velocity Average Path (VAP) was assessed using a Computer Assisted Sperm Analysis (CASA) system. At 20 degrees C, the first movements of the trochophores were observed at 6.5 h post-fertilization. The mean (+/- SD) percentage of swimming trochophores and the VAP observed... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00157/26786/24940.pdf |
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| Suquet, Marc; Quere, Claudie; Mingant, Christian; Lebrun, Luc; Ratiskol, Dominique; Miner, Philippe; Cosson, Jacky. |
| Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (YAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, YAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed... |
| Tipo: Text |
Palavras-chave: Spermatozoa; CASA; Maturation; Sperm motility; Bivalve; Pecten maximus. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00157/26852/24983.pdf |
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| Holbach, Marine; Robert, Rene; Miner, Philippe; Mingant, Christian; Boudry, Pierre; Tremblay, Rejean. |
| Hatchery production of great scallop, Pecten maximus, remains unpredictable, notably due to poor larval survival. Large-scale flow-through systems up to 3500 L have been developed to avoid the use of antibiotics in static systems. Alternatively, small-scale flow-through systems have been successfully applied for oysters but they proved to be unsuitable to rear scallop larvae. By focusing on physical factors presumed to limit P. maximus larval development, this study aimed to optimize great scallop larvae rearing parameters under controlled conditions. First, the influence of aeration on larval performances, energetic metabolism and antioxidant defences were studied both in static and flow-through systems. Aeration depressed larval food intake, regardless... |
| Tipo: Text |
Palavras-chave: Aeration; Flow-through; Larval physiology; Pecten maximus. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00384/49508/51151.pdf |
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| Gonzalez, Ricardo; Mingant, Christian; Petton, Bruno; Robert, Rene. |
| In contrast with the Japanese oyster Crassostrea gigas and the Manila clam Ruditapes philippinarum, Ostrea edulis seed production in the hatchery has been reported to be erratic, with sudden and unexplained larval and post-metamorphosis mortalities. Fecundity and initial larval quality have been related to broodstock conditioning, but effects on larval development and metamorphosis remain poorly understood. In addition, molluscan larval mortalities have been often associated with bacterial contamination and flow-through techniques may help to overcome this problem. Both aspects have been considered in the present work. O. edulis broodstock were conditioned at 19 °C and fed three different microalgal diets. Two were single-species diets: Rhodomonas salina... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Feeding; Broodstock conditioning; Larval development; Flow-through. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00090/20093/17732.pdf |
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| Da Costa, F.; Petton, Bruno; Mingant, Christian; Bougaran, Gael; Rouxel, Catherine; Quere, Claudie; Wikfors, G. H.; Soudant, P.; Robert, Rene. |
| Effects of a remarkably high overall lipid Tisochrysis lutea strain (T+) upon gross biochemical composition, fatty acid (FA), sterol and lipid class composition of Crassostrea gigas larvae were evaluated and compared with a normal strain of Tisochrysis lutea (T) and the diatom Chaetoceros neogracile (Cg). In a first experiment, the influence of different single diets (T, T+ and Cg) and a bispecific diet (TCg) was studied, whereas, effects of monospecific diets (T and T+) and bispecific diets (TCg and T+Cg) were evaluated in a second experiment. The strain T+ was very rich in triglycerides (TAG: 93–95% of total neutral lipids), saturated FA (45%), monounsaturated FA (31–33%) and total fatty acids (4.0–4.7 pg cell−1). Larval oyster survival and growth rate... |
| Tipo: Text |
Palavras-chave: Larvae; Lipids; Oyster; Tisochrysis lutea. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00251/36258/36063.pdf |
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| Rico Villa, Benjamin; Le Coz, Jean-rene; Mingant, Christian; Robert, Rene. |
| Microalgae commonly used as feed for bivalves, Pavlova lutheri (P), Isochrisis affinis galbana (T) and Chaetoceros calcitrans forma pumilum (Cp), were fed to Pacific oyster Crassostrea gigas to assess their nutritional value for larval development and metamorphosis during two experiments. Monospecific, bispecific and trispecific diets were firstly evaluated during 3 weeks from D larvae to young postlarvae. Then bispecific diets, based on different T and Cp proportions, were assessed during a similar period. Concurrently, ingestion was studied through the whole larval and postlarval development for each diet and/or diet mixture. Because lipids are assumed to be a key nutrient for bivalves, biochemical analysis was undertaken on the second set of trials... |
| Tipo: Text |
Palavras-chave: Lipids; Grazing; Metamorphosis; Growth; Larvae; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1707.pdf |
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| Dao, Jean-claude; Barret, Jean; Carval, Jean-pierre; Connan, Jean-paul; Corre, Christian; Devauchelle, Nicole; Fleury, Pierre-gildas; Laurent, Pascale; Mazuret, Michel; Miner, Philippe; Mingant, Christian; Muzellec, Marie-louise; Robert, Rene. |
| Aquaculture production of scallop has been identified by a series of technical operations during the past ten years programme under a partnership involving the fishermen organizations with regional and national grants. 3 million juveniles have been produced annually, giving a potential recapture by fishing of 100 to 120 tonnes 3 years later. Results in terms of technical and economical approach have pointed out the principal unknown factors responsable for high costs. 7he pilot programme aims to increase the production to reach a level of 5 million juveniles to support the fishery of the rade de Brest and induce new management of the resources. Two priorities were selected, control of the reproduction in the hatchery to obtain reliable larval and... |
| Tipo: Text |
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| Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00044/15494/12860.pdf |
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| Suquet, Marc; Malo, Florent; Gourtay, Clemence; Ratiskol, Dominique; Riobe, Marion; Le Grand, Jacqueline; Queau, Isabelle; Mingant, Christian. |
| A basic cryopreservation protocol for great scallop sperm was recently published, with which thawed sperm survival remained limited to 25% of control values (Suquet et al. 2014). We designed here a set of five experiments to test ways of improving thawed sperm survival and storage by studying the effects of (1) cryoprotectant concentration; (2) sugar incorporation in the extender; (3) egg yolk addition to the extender; (4) sperm dilution rate; and (5) storage capacity of thawed sperm at 4 degrees C. A sixth experiment was then performed to compare rearing performances of larvae produced using thawed sperm from the optimal protocol defined in the present study with those produced using fresh sperm. During the first experiment, a significantly higher... |
| Tipo: Text |
Palavras-chave: Pecten maximus; Mollusc; Sperm; Cryopreservation; Larval rearing. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00359/47033/46946.pdf |
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| Sussarellu, Rossana; Suquet, Marc; Thomas, Yoann; Lambert, Christophe; Fabioux, Caroline; Pernet, Marie Eve Julie; Le Goic, Nelly; Quillien, Virgile; Mingant, Christian; Epelboin, Yanouk; Corporeau, Charlotte; Guyomarch, Julien; Robbens, Johan; Paul-pont, Ika; Soudant, Philippe; Huvet, Arnaud. |
| Plastics are persistent synthetic polymers that accumulate as waste in the marine environment. Microplastic (MP) particles are derived from the breakdown of larger debris or can enter the environment as microscopic fragments. Because filter-feeder organisms ingest MP while feeding, they are likely to be impacted by MP pollution. To assess the impact of polystyrene microspheres (micro-PS) on the physiology of the Pacific oyster, adult oysters were experimentally exposed to virgin micro-PS (2 and 6 µm in diameter; 0.023 mg·L−1) for 2 mo during a reproductive cycle. Effects were investigated on ecophysiological parameters; cellular, transcriptomic, and proteomic responses; fecundity; and offspring development. Oysters preferentially ingested the 6-µm micro-PS... |
| Tipo: Text |
Palavras-chave: Microplastic; Reproduction; Energy allocation; Oyster. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00311/42233/41600.pdf |
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| Santerre, Christelle; Sourdaine, Pascal; Marc, Nicolas; Mingant, Christian; Robert, Rene; Martinez, Anne-sophie. |
| The sex-determining system of Crassostrea gigas is still poorly known, especially regarding the potential influence of temperature. In order to address this question, mRNA expressions of actors of the molecular cascade (Cg-DMI, Cg-SoxE, Cg-beta-catenin, Cg-Foxl2/Cg-FoxI2os) and of Oyvlg, a germ cell marker, were investigated by real-time PCR in spat grown at different temperatures (18, 22, 25 and 28 degrees C). In parallel, gonadic differentiation, gametogenesis and sex ratios were assessed by histology at each of these temperatures. Whatever the temperature, Cg-DMI, Cg-SoxE, Cg-beta-catenin and Oyvlg expressions peaked at the same developmental stage, always after Cg-Foxl2/Cg-Foxl2os (around 40-44 dpf for spat grown at 18 degrees C). Temperatures... |
| Tipo: Text |
Palavras-chave: Oyster spat; Temperature; Sex ratio; TSD; Sex determination pathway. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00136/24767/22874.pdf |
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| Fabioux, Caroline; Huvet, Arnaud; Lelong, C; Robert, Rene; Pouvreau, Stephane; Daniel, Jean-yves; Mingant, Christian; Le Pennec, M. |
| The oyster vasa-like gene was previously demonstrated to be specifically expressed in germline cells of adult oysters Crassostrea gigas. In the present study, this gene was used as a molecular marker to establish the developmental pattern of germline cells during oyster ontogenesis. using whole-mount in situ hybridization and real-time PCR. The Oyvlg transcripts appeared to be localized to the vegetal pole of unfertilized oocytes and maternally transmitted to embryos. At early development, these maternal transcripts were observed to segregate into a single blastomere, from the CD macromere of 2-cell stage to the 4d mesentoblast of blastula. From late blastula stage, the mesentoblast divided into two cell clumps that migrated to both sides of the larvae... |
| Tipo: Text |
Palavras-chave: Primordial germ cells; Oyster vasa like gene; Germline; Development; Crassostrea gigas. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-648.pdf |
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| Registros recuperados: 29 | |
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