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| Moraes,C.M.; Conceição,F.R.; Rocha,A.S.R.; Santos Júnior,A.G.; Ribas,L.M.; Vargas,A.P.C.; Nogueira,C.E.W.; Gil-Turnes,C.; Leite,F.P.L.. |
| Strangles is an economically important horse disease caused by Streptococcus equi subsp. equi. The diagnosis can be confirmed either directly by bacterial isolation and PCR or by ELISA, which is an indirect method based on the detection of serum antibodies. The aim of this study was to clone, express and characterize the SeM protein of Streptococcus equi subsp. equi, evaluate its use as antigen in indirect ELISA and determine its performance to distinguish sera of negative, vaccinated and positive animals. This was initially performed by cloning the gene encoding the SeM protein and its expression in Escherichia coli. Subsequently, the protein produced was characterized and used as antigen in ELISA. Serum samples for evaluation were taken from 40 negative... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Strangles Streptococcus equi; SeM ELISA. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000401015 |
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| Maciel,L.F.; Magalhães,C.G.; Moraes,C.M.; Rosa,M.C.; Mendonça,M.; Moreira,A.N.; Dummer,L.A.; Finger,P.F.; Ferreira,M.R.A.; Leite,F.P.L.; Conceição,F.R.. |
| ABSTRACT The strangles is an infectious disease that affects horses from all ages and causes important economic losses in the equine-related business. The aim of this work was to evaluate the immunogenicity of the recombinant M protein from Streptococcus equi (rSeM) co-administered with the recombinant heat-labile enterotoxin B subunit from Escherichia coli (rLTB) in mice and horses. A total of 72 female Balb-c mice were divided into eight groups and 18 horses were divided into six groups. The animals were inoculated by intramuscular (IM) or intranasal (IN) routes with different treatments of rSeM, rLTB and/or Al(OH)3. The results obtained in both species, independent of administration routes, demonstrated that rSeM + rLTB had higher levels of specific... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Garrotilho; Streptococcus equi; RSeM e rLTB. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501351 |
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| Gonçalves,E.P.M.; Barros,M.C.S.; Pessôa,M.C.; Cardilli,D.J.; Roos,T.B.; Moraes,C.M.. |
| RESUMO O objetivo deste trabalho foi padronizar uma PCR para a detecção do Salmo salar, a qual possa ser usada na autenticação do salmão utilizado em pratos da culinária japonesa e do pescado comercializado in natura. Para isso, dois lotes de sushi foram produzidos experimentalmente. Além disso, foram visitados 38 estabelecimentos que comercializam comida japonesa e 10 peixarias na região metropolitana de Belém, visando à coleta do sushi, do temaki e do pescado pertencente à espécie Salmo salar. Os dados demonstraram que a técnica foi eficiente para a autenticação de Salmo salar, visto que a espécie foi detectada tanto nas amostras de sushis preparados experimentalmente quanto nas alíquotas de pescados isolados, utilizados para a preparação do sushi. Em... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sushi; Pescado; Temaki; Identificação; DNA. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200640 |
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