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| Suganuma, Keisuke; Yamasaki, Shino; Molefe, Nthatisi Innocentia; Musinguzi, Peter Simon; Davaasuren, Batdorj; Mossaad, Ehab; Narantsatsral, Sandagdorj; Battur, Banzragch; Battsetseg, Badgar; Inoue, Noboru. |
| Dourine is caused by Trypanosoma equiperdum via coitus with an infected horse. Although dourine is distributed in Equidae worldwide and is listed as an internationally important animal disease by the World Organization for Animal Health (OIE), no effective treatment strategies have been established. In addition, there are no reports on drug discovery, because no drug screening system exists for this parasite. A new T.?equiperdum strain was recently isolated from the genital organ of a stallion that showed typical symptoms of dourine. In the present study, we adapted T.?equiperdum IVM-t1 from soft agarose media to HMI-9 liquid media to develop a drug screening assay for T.?equiperdum. An intracellular ATP-based luciferase assay using CellTiter-Glo reagent... |
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Palavras-chave: Colorimetric assay; Drug screening system; Liquid culture; Luciferase assay; Trypanosoma equiperdum. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4538 |
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| Mossaad, Ehab; Salim, Bashir; Suganuma, Keisuke; Musinguzi, Peter; Hassan, Mohammed A.; Elamin, E. A.; Mohammed, G. E.; Bakhiet, Amel O.; Xuan, Xuenan; Satti, Rawan A.; Inoue, Noboru. |
| Background: This study was conducted in response to recurring reports from eastern Sudan of camel trypanosomosis that can no longer be treated by currently available trypanocidal drugs. One hundred and eighty-nine blood samples were obtained from camels in different herds and local markets in the western part of Sudan, and a cross-sectional study was carried out between December 2015 and February 2016 to identify the causative agents and possible circulating genotypes. Results: The prevalence of trypanosomes detected using the conventional parasitological techniques of Giemsa-stained blood smears, wet blood smears and the microhematocrit centrifugation technique (MHCT) was 7% (13/189), 11% (21/189) and 19% (36/189), respectively. However, a multi-species... |
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Palavras-chave: Dromedary camels; Sudan; Trypanosomosis; Trypanosoma evansi; Trypanosoma vivax. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4467 |
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