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A novel expression vector for the secretion of abaecin in Bacillus subtilis BJM
Li,Li; Mu,Lan; Wang,Xiaojuan; Yu,Jingfeng; Hu,Ruiping; Li,Zhen.
ABSTRACT This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter Pglv, and a mature beta-glucanase signal peptide. Also, a B. subtilis expression system was constructed. The recombinant abaecin gene was expressed and purified as a recombinant protein in the culture supernatant. The purified abaecin did not inhibit the growth of Escherichia coli strain K88. Cecropin A and hymenoptaecin exhibited potent bactericidal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abaecin; Antimicrobial peptides; Bacillus subtilis; Expression.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400809
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Soil respiration in cucumber field under crop rotation in solar greenhouse Scientia Agricola
Liang,Yinli; Bai,Caihong; Mu,Lan; Zhou,Maojuan.
Crop residues are the primary source of carbon input in the soil carbon pool. Crop rotation can impact the plant biomass returned to the soil, and influence soil respiration. To study the effect of previous crops on soil respiration in cucumber (Cucumis statirus L.) fields in solar greenhouses, soil respiration, plant height, leaf area and yield were measured during the growing season (from the end of Sept to the beginning of Jun the following year) from 2007 to 2010. The cucumber was grown following fallow (CK), kidney bean (KB), cowpea (CP), maize for green manure (MGM), black bean for green manure (BGM), tomato (TM), bok choy (BC). As compared with CK, KB, CP, MGM and BGM may increase soil respiration, while TM and BC may decrease soil respiration at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leaf area; CO2 emission; Plant height; Previous crop; Yield.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000400012
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Temporal and spatial variation of soil respiration under mulching in a greenhouse cucumber cultivation PAB
Mu,Lan; Fang,Lan; Liang,YinLi.
Abstract: The objective of this work was to determine the temporal and spatial variations of soil respiration (Rs) in a greenhouse, as well as the factors causing them, in a soil covered with different mulching types and cultivated with cucumber (Cucumis sativus). Four mulching treatments were applied to a soil cultivated with cucumber: CK, conventional practice without mulch; FM, plastic film mulch; CM, combined mulch of plastic film with wheat straw; and SM, wheat straw mulch. Rs was measured by a soil respiration chamber connected to a portable infrared gas analyzer, and the differences of Rs between mulchings were evaluated. Rs was significantly lower in the control than in the treatments with mulch. In addition, Rs was higher in the position on the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cucumis sativus; Climate change; Soil CO2 emission; Soil quality..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000700869
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