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Influence of inoculum density of the antagonistic bacteria Pseudomonas fluorescens and Pseudomonas corrugata on sugar beet seedling colonisation and suppression of Pythium damping off Organic Eprints
Schmidt, C. S.,; Agostini, F.,; Leifert, C.,; Killham, K.,; Mullins, C. E.,.
The effect of initial inoculum density of the antagonistic bacterial strains Pseudomonas fluorescens 135 and Pseudomonas corrugata 2140 (10(3) to 10(8) CFU per seed pellet) on sugar beet seedling colonisation, in situ bioluminescence and antagonistic activity towards Pythium ultimum was investigated. Populations of the bacteria colonising sugar beet root systems approached an apparent carrying capacity of 10(5) to 10(6) CFU per plant after 12 d growth, irrespective of inoculum density. This meant an up to 320-fold population increase at low inoculum densities and a decrease at high densities. Population densities of both bacteria and their corresponding in situ bioluminescence (resulting from luciferase enzyme expression from the inserted luxAB genes)...
Tipo: Journal paper Palavras-chave: Crop health; Quality; Protection Soil biology.
Ano: 2004 URL: http://orgprints.org/10763/1/Schmidt_et_al_inoculum_2004.pdf
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Low temperature-short duration steaming of soil kills soil-borne pathogens, nematode pests and weeds Organic Eprints
van Loenen, M. C. A.,; Turbett, Y.,; Mullins, C. E.,; Feilden, N. E. H.,; Wilson, M. J.,; Leifert, C.,; Seel, W. E.,.
Agricultural soil samples containing survival structures of the fungal crop pathogens Verticillium dahliae, Sclerotinia sclerotiorum, Sclerotium cepivorum, Pythium ultimum, potato cyst nematodes Globodera rostochiensis and G. pallida and weeds Chenopodium album and Agropyron repens [Elymus repens] were treated in the laboratory with aerated steam at temperatures ranging from 40 to 80degreesC in a specially constructed apparatus. Steaming at 50 or 60degreesC for 3 min, followed by an 8-min resting period in the steamed soil and immediate removal from the soil thereafter, resulted in 100% kill of all weeds, diseases and nematodes. When steamed at 45degreesC, there was a small but significant reduction in the survival of V. dahliae microsclerotia but no...
Tipo: Journal paper Palavras-chave: Crop health; Quality; Protection Weed management Soil biology.
Ano: 2003 URL: http://orgprints.org/10762/1/van_loenen_et_al_2003.pdf
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