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| Muroi, Yoshikage; Ishii, Toshiaki. |
| Animals consume energy for reproduction, as well as survival. Excess or insufficient energy investment into reproduction, respectively, threatens the survival of parents or leads to the failure of reproduction. Management of energy consumption in reproduction is important, not only for the success of the process, but also for the survival of the parents. Reproductive behaviors, such as mating and parental behavior, are indispensable for achieving each event of reproduction including gametogamy, parturition, and lactation. Therefore, reproductive behavior is one of the important factors in managing energy consumption for reproduction. Orexigenic and anorexigenic molecules in the hypothalamus have been implicated in the regulation of reproductive functions.... |
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Palavras-chave: Neuropeptide Y; Hypothalamus; Dorsal raphe nucleus; Reproductive behavior. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4530 |
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| Ishii, Toshiaki; Suenaga, Ryuta; Iwata, Wataru; Miyata, Ryouhei; Fujikawa, Ryu; Muroi, Yoshikage. |
| The mesencephalic trigeminal sensory nucleus (Me5), which receives signals originating from oral proprioceptors, becomes active at weaning and contributes to the acquisition of active exploratory behavior [Ishii, T., Furuoka, H., Kitamura, N., Muroi, Y., and Nishimura, M. (2006) Brain Res. 1111, 153-161]. Because cognitive functions play a key role in animal exploration, in the present study we assessed the role of Me5 in spatial learning and memory in the water maze. Mice with bilateral Me5 lesions exhibited severe deficits in both a reversal learning and a reversal probe test compared with sham-operated mice. In spite of these reversal tests, Me5 lesions had no effect on a hidden platform test. These results suggest that Me5-lesioned mice show a... |
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Palavras-chave: Spatial learning and memory; Me5 lesions; Hippocampal neurogenesis; Dentate gyrus. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2824 |
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| Ihara, Fumiaki; Nishimura, Maki; Muroi, Yoshikage; Furuoka, Hidefumi; Yokoyama, Naoaki; Nishikawa, Yoshifumi. |
| Neospora caninum is an obligate intracellular parasite that causes neurological disorders in dogs and cattle. The majority of host animals are asymptomatic at the chronic stage of infection. However, it remains unclear whether cerebral function is normal in asymptomatic animals. In this study, mice were infected with N. caninum (strain Nc-1) and their brains were examined to understand changes in cerebral function at the chronic stage of infection. Mice infected with N. caninum showed impaired locomotor activity, but no differences in clinical symptoms were observed. In the brains of infected mice, parasites were distributed throughout the brain and histological lesions were observed everywhere except for the cerebellum. Expression levels of... |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4460 |
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| Ishii, Toshiaki; Furuoka, Hidefumi; Muroi, Yoshikage; Nishimura, Masakazu; 石井, 利明; 古岡, 秀文; 室井, 喜景. |
| Integrin-linked kinase (ILK) is a focal adhesion serine/ threonine protein kinase with an important role in integrin and growth factor signaling pathways. Recently, we demonstrated that ILK is expressed in N1E-115 neuroblastoma cells and controls integrin-dependent neurite outgrowth in serum-starved cells grown on laminin (Ishii, T., Satoh, E., and Nishimura, M. ( 2001) J. Biol. Chem. 276, 42994 - 43003). Here we report that ILK controls tau phosphorylation via regulation of glycogen synthase kinase-3beta (GSK-3beta) activity in N1E-115 cells. Stable transfection of a kinase-deficient ILK mutant (DN-ILK) resulted in aberrant tau phosphorylation in N1E-115 cells at sites recognized by the Tau-1 antibody that are identical to some of the phosphorylation... |
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Palavras-chave: ALZHEIMERS-DISEASE NEURITE OUTGROWTH PROTEIN; NEURONS HYPERPHOSPHORYLATION KINASE-3-BETA ACCUMULATION BINDING ROLES. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/747 |
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| Fujikawa, Ryu; Muroi, Yoshikage; Unno, Toshihiro; Ishii, Toshiaki; 室井, 喜景; 石井, 利明. |
| Botulinum neurotoxin serotype A (BoNT/A) inhibits acetylcholine release at the neuromuscular junction in isolated muscles, and ouabain can partially block its effect. However, it is not clear whether ouabain attenuates BoNT/A-induced neuromuscular paralysis in vivo. In this work, we investigated the effects of ouabain on BoNT/A-induced neuromuscular paralysis in mice. Ouabain was administered to mice intraperitoneally immediately after a single injection of BoNT/A into skeletal muscle. The effects of ouabain on BoNT/A-induced muscle paralysis were assessed by quantitative monitoring of muscle tension and digit abduction via the digit abduction scoring (DAS) assay. A single administration of ouabain significantly prolonged BoNT/A-induced neuromuscular... |
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Palavras-chave: Ouabain; Botulinum neurotoxin; Muscle atrophy; MAFbx; MuRF-1. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3123 |
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| Kinoshita, Ken-ichi; Muroi, Yoshikage; Unno, Toshihiro; Ishii, Toshiaki. |
| Cognitive impairment often occurs in Parkinson's disease (PD), but the mechanism of onset remains unknown. Recently, we reported that PD model mice produced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) show facilitation of hippocampal memory extinction, which may be the cause of cognitive impairment in PD. When we examined the cAMP/CREB signaling in the hippocampus, decreased levels of cAMP and phosphorylated CREB were observed in the dentate gyrus (DG) of MPTP-treated mice. Administration of rolipram improved the memory deficits with concomitant recovery of cAMP and phosphorylated CREB levels, suggesting that reduced cAMP/CREB signaling in the DG leads to cognitive impairment in MPTP-treated mice. (C) 2017 The Authors. Production and hosting by... |
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Palavras-chave: Memory extinction; MPTP-Treated mice; Phosphodiesterase IV inhibitor rolipram. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4547 |
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| Ishii, Toshiaki; Furuoka, Hidefumi; Kitamura, Nobuo; Muroi, Yoshikage; Nishimura, Masakazu; 古岡, 秀文; 北村, 延夫; 室井, 喜景; 石井, 利明. |
| Post-weaning mice fed exclusively milk display low-frequency exploratory behavior [Ishii, T., Itou, T., and Nishimura, M. (2005) Life Sci. 78, 174-179] compared to mice fed a food pellet diet. This low-frequency exploratory behavior switched to high-frequency exploration after a switch from exclusively milk formula to a food pellet diet. Acquisition of the high-frequency exploratory behavior was irreversible. Recently, we demonstrated that the mesencephalic trigeminal nucleus (Me5) is involved in the control of feeding and exploratory behavior in mice without modulating the emotional state [Ishii, T., Furuoka, H., Itou, T., Kitamura, N., and Nishimura, M. (2005) Brain Res. 1048, 80-86]. We therefore investigated whether the Me5 is involved in acquisition... |
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Palavras-chave: Exploratory behavior; Me5 lesions; Weaning. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/739 |
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| Ihara, Fumiaki; Nishimura, Maki; Muroi, Yoshikage; Mahmoud, Motamed Elsayed; Yokoyama, Naoaki; Nagamune, Kisaburo; Nishikawa, Yoshifumi. |
| Chronic infection with Toxoplasma gondii becomes established in tissues of the central nervous system, where parasites may directly or indirectly modulate neuronal function. Epidemiological studies have revealed that chronic infection in humans is a risk factor for developing mental diseases. However, the mechanisms underlying parasite-induced neuronal dysfunction in the brain remain unclear. Here, we examined memory associated with conditioned fear in mice and found that T. gondii infection impairs consolidation of conditioned fear memory. To examine the brain pathology induced by T. gondii infection, we analyzed the parasite load and histopathological changes. T. gondii infects all brain areas, yet the cortex exhibits more severe tissue damage than other... |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4480 |
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| Muroi, Yoshikage; Ishii, Toshiaki; Komori, Seiichi; Kitamura, Nobuo; Nishimura, Masakazu; 室井, 喜景; 石井, 利明; 北村, 延夫. |
| We previously reported that male mice are more attracted to volatile odors from intact female mice than from ovarlectornized female mice. In the present study, we investigated male attraction to volatile odors from soiled bedding collected from the cages of estrous or ovariectomized female mice. There was no difference in the total time spent sniffing volatile odors from estrous and ovariectomized female mice, suggesting that female mice emit volatile odors which are not excreted into bedding. To test this possibility, we investigated c-Fos expression in the mitral cell layer and granule cell layer of the accessory olfactory bulb 60 min after exposure of male mice to volatile odors without physical contact. Volatile odors from an estrous female mouse... |
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Palavras-chave: Accessory olfactory bulb; Vomeronasal organ; Pheromone; C-Fos; Habituation-dishabituation test; Sexual attraction. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/45 |
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