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| Registros recuperados: 24 | |
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| SILVA, J. P.; TICONA, A. R. P.; HAMANN, P. R. V.; QUIRINO, B. F.; NORONHA, E. F.. |
| Lignocellulosic residues are low-cost abundant feedstocks that can be used for industrial applications. However, their recalcitrance currently makes lignocellulose use limited. In natural environments, microbial communities can completely deconstruct lignocellulose by synergistic action of a set of enzymes and proteins. Microbial degradation of lignin by fungi, important lignin degraders in nature, has been intensively studied. More recently, bacteria have also been described as able to break down lignin, and to have a central role in recycling this plant polymer. Nevertheless, bacterial deconstruction of lignin has not been fully elucidated yet. Direct analysis of environmental samples using metagenomics, metatranscriptomics, and metaproteomics approaches... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Lignina; Biodegradação; Lignin; Biodegradation; Metagenomics; Proteomics; Transcriptomics. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131423 |
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| TAMIETTI, M. S.; MIDORIKAWA, G. E. O.; MENDES, T. D.; DAMASO, M. C. T.; BOM, E. P. S.; SILVA, A. S.; GOTTSCHALK, L. M. F.; NORONHA, E. F.; FAVARO, L. C. de L.. |
| Carbon catabolite repression (CCR) is a mechanism by which microorganisms can utilize preferably highly energetic compounds over those of difficult degradation. For Trichoderma reesei, the protein that acts as repressor in the presence of glucose is CRE1. In this project, we aim to delete cre1 gene in Trichoderma harzianum CFAM-422 and obtain mutants with enhanced production of biomass degrading enzymes. Disruption of cre1 in T. harzianum CFAM-422 was performed by gene replacement of cre1 for hph (hygromycin B phosphotransferase) via homologous recombination. Hygromycin resistant mutants and parental strains enzyme production was evaluated in both inductive and repressive conditions in four different carbon sources. Enzymatic indexes (EI) were determined... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Celulase; Processos de hidrolise enzimática de biomassa; Trichoderma harzianum; Carbon Catabolite Repression; Cellulolytic and hemicellulolytic enzymes. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076994 |
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| ANDRADE, A. E.; FELIX, G. C.; OLIVEIRA, A. C.; NORONHA, E. F.; PEREIRA, J. L.; LIMA, L. H. C.; ROSATO, Y. B.; MELO, J. A. T.; BLOCH JUNIOR, C.; MEHTA, A.. |
| bitstream/CENARGEN/26686/1/bp093.pdf |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Xanthomonas campestris pv campestris; Brassica olearacea; Expressão Genética; Borecole; Plant hostess; Genetic expression.; Bactéria; Couve; Podridão Negra.; Planta Hospedeira. |
| Ano: 2005 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/187100 |
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| SOUTO, B. de M.; ARAÚJO, A. C. B. de; HAMANN, P. R. V.; BASTOS, A. de R.; CUNHA, I. de S.; PEIXOTO, J.; KRUGER, R. H.; NORONHA, E. F.; QUIRINO, B. F.. |
| Functional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase,... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Enzima; Clonagem; Programa de Computador; Peptídeo; Metagenomics; Enzymes; Cloning (animals); Signal peptide; Prevotella; Computer software; Screening. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129624 |
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| SILVA, D. P.; CASADO-FILHO, E. L.; CORRÊA, A. S. R.; FARIAS, L. R.; BLOCH JUNIOR, C.; GROSSI DE SÁ, M. F.; MENDES, P. A. M.; QUIRINO, B. F.; NORONHA, E. F.; FRANCO, O. L.. |
| bitstream/item/178141/1/ID-28054-1.PDF |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Defesa da planta; Inibidor alpha-amilase; Callosobruchus Maculatus; Pterodon Pubescens. |
| Ano: 2007 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/188418 |
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| ANDRADE, A. E.; SILVA, L. P.; PEREIRA, J. L.; NORONHA, E. F.; REIS JÚNIOR, F. B. R.; BLOCH JÚNIOR, C.; SANTOS, M. F. dos; DOMONT, G. B.; FRANCO, O. L.; MEHTA, A.. |
| bitstream/item/177330/1/SP-19557-ID-30489.pdf |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Brassica Oleracea; Podridão Negra; Xanthomonas Campestris. |
| Ano: 2008 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/169382 |
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| FARIAS, L. R.; COSTA, F. T.; SOUZA, L. A.; PELEGRINI, P. B.; GROSSI DE SÁ, M. F.; NETO, S. M.; BLOCH JÚNIOR, C.; LAUMANN, R. A.; NORONHA, E. F.; FRANCO, O. L.. |
| bitstream/item/178146/1/ID-28707-1.pdf |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Inibidor alfa-amilase; Defesa da planta; Callosobruchus Maculatus; Carica Papaya; Mamão. |
| Ano: 2007 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/188925 |
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| ANDRADE, A. E.; FELIX, G. C.; OLIVEIRA, A. C.; NORONHA, E. F.; PEREIRA, J. L.; LIMA, L. H. C.; ROSATO, Y. B.; VIDAL, M. C.; MELO, J. A. T.; BLOCH JÚNIOR, C.; MEHTA, A.. |
| 2005 |
| Tipo: Artigo em anais de congresso (ALICE) |
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| Ano: 2005 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/187110 |
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| Registros recuperados: 24 | |
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