|
|
|
|
|
| Souza,Viviane; Nader Filho,Antonio; Melo,Poliana de Castro; Ferraudo,Guilherme Moraes; Ferraudo,Antônio Sérgio; Conde,Sandra de Oliveira; Fogaça Junior,Flavio Augusto. |
| The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples' collection sites with the highest isolation frequency of Staphylococcus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mastitis; Staphylococcus aureus; Bulk tank; PCR; PFGE. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400050 |
| |
|
|
| Fagundes,Helena; Barchesi,Luciana; Nader Filho,Antonio; Ferreira,Luciano Menezes; Oliveira,Carlos Augusto Fernandes. |
| The objective of the present study was to evaluate the occurrence of Staphylococcus aureus in milk produced in 37 farms located in the regions of Ribeirão Preto and São Carlos, state of São Paulo, Brazil. Two-hundred and eight samples of milk from individual cows showing subclinical mastitis, and 37 samples of bulk tank milk were analyzed. S. aureus strains were detected in 18 (7.3%) milk samples: 14 (6.7%) from samples of individual cows, and 4 (10.8%) from bulk tank milk. Two individual milk samples (14.3%) and two bulk milk samples contained enterotoxigenic S. aureus. PFGE analysis revealed the genetic heterogeneity of the strains isolated from raw milk, which presented to 13 S. aureus patterns. Results confirmed the potential transmission of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; Raw milk; Bovine; Mastitis; Enterotoxin. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200018 |
| |
|
|
| Ferreira,Luciano Menezes; Nader Filho,Antonio; Oliveira,Erika de; Zafalon,Luiz Francisco; Souza,Viviane de. |
| Foram submetidas a PCR-Ribotipagem e aos testes de sensibilidade in vitro frente a 12 antimicrobianos 77 estirpes de Staphylococcus aureus isoladas em amostras de leite procedentes de 40 vacas da raça holandesa que apresentaram mastite subclínica, em uma propriedade rural localizada no Estado de São Paulo, Brasil. Os resultados obtidos revelaram quatro diferentes padrões de resistência a antimicrobianos, sendo observada a predominância de resistência à lincomicina entre 19 (24,7%) estirpes de S.aureus. As 58 (75,3%) estirpes restantes foram sensíveis aos 12 antimicrobianos testados. A PCR-ribotipagem revelou a ocorrência de nove padrões genotípicos distintos, além de apresentar uma capacidade discriminatória maior (D = 0,82) que a obtida nos antibiogramas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mastite; Staphylococcus aureus; Variabilidade fenotípica e genotípica. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000400028 |
| |
|
|
|
