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Registros recuperados: 19
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Abundant immunohistochemical expression of dopamine D2 receptor and p53 protein in meningiomas: follow-up, relation to gender, age, tumor grade, and recurrence BJMBR
Trott,G.; Pereira-Lima,J.F.S.; Leães,C.G.S.; Ferreira,N.P.; Barbosa-Coutinho,L.M.; Oliveira,M.C..
Meningiomas are common, usually benign tumors, with a high postoperative recurrence rate. However, the genesis and development of these tumors remain controversial. We aimed to investigate the presence and implications of a mutated p53 protein and dopamine D2 receptor in a representative series of meningiomas and to correlate these findings with age, gender, tumor grade, and recurrence. Tumor tissue samples of 157 patients diagnosed with meningioma (37 males and 120 females, mean age 53.6±14.3 years) who underwent surgical resection between 2003 and 2012 at our institution were immunohistochemically evaluated for the presence of p53 protein and dopamine D2 receptor and were followed-up to analyze tumor recurrence or regrowth. Tumors were classified as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Receptors; Dopamine D2; Immunohistochemistry; Meningioma; Tumor suppressor protein p53; Recurrence.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500415
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Acetolactate synthase activity in Euphorbia heterophylla resistant to ALS- and protox- inhibiting herbicides Planta Daninha
Xavier,E.; Oliveira,M.C.; Trezzi,M.M.; Vidal,R.A.; Diesel,F.; Pagnoncelli,F.D.; Scalcon,E..
The objective of this study was to determine the activity of the enzyme acetolactate synthase in biotypes of wild poinsettia (Euphorbia heterophylla) with multiple resistance to ALS- and Protox- inhibitors in the presence and absence of imazapyr, imazethapyr and nicosulfuron. We conducted in vitro assay of ALS enzyme extracted from plants of Vitorino, Bom Sucesso do Sul and Medianeira biotypes (with multiple resistance) and a susceptible population in the absence and presence of imazapyr, imazethapyr and nicosulfuron. In the absence of herbicides, biotypes with multiple resistance showed higher affinity for the substrate of the enzyme compared with the susceptible population. The herbicides imazapyr, imazethapyr and nicosulfuron had little effect on the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cross resistance; Wild poinsettia; Mechanism of resistance.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582013000400013
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Chronic effect of antidopaminergic drugs or estrogen on male Wistar rat lactotrophs and somatotrophs BJMBR
Oliveira,M.C.; Messinger,H.P.; Tannhauser,M.; Barbosa-Coutinho,L..
The aim of the present study was to evaluate the effect of antidopaminergic agents on the somatotrophs in the presence of hyperprolactinemia. Adult male Wistar rats were divided into 6 groups: a control group and five groups chronically treated (60 days) with haloperidol, fluphenazine, sulpiride, metoclopramide or estrogen. Somatotrophs and lactotrophs were identified by immunohistochemistry and the data are reported as percent of total anterior pituitary cells counted. The drugs significantly increased the percentage of lactotrophs: control (mean ± SD) 21.3 ± 4.4, haloperidol 27.8 ± 2.2, fluphenazine 34.5 ± 3.6, sulpiride 32.7 ± 3.5, metoclopramide 33.4 ± 5.5 and estrogen 42.4 ± 2.8. A significant reduction in somatotrophs was observed in animals treated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Somatotrophs; Lactotrophs; Estrogen; Antidopaminergic drugs.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200018
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Desempenho, características de carcaça e viabilidade econômica de coelhos sexados abatidos em diferentes idades Arq. Bras. Med. Vet. Zootec.
Oliveira,M.C.; Lui,J.F..
Avaliaram-se o desempenho, as características de carcaça e a viabilidade econômica de coelhos sexados abatidos em diferentes idades. Foram usados 100 coelhos (50 machos e 50 fêmeas), em delineamento de blocos inteiramente ao acaso e arranjo fatorial 2×2 (dois sexos e duas idades ao abate), com quatro tratamentos e cinco repetições. Os coelhos foram desmamados aos 35 e abatidos aos 75 ou 90 dias de idade. Não houve diferença entre sexo (P>0,05) nos parâmetros avaliados. Os animais abatidos aos 90 dias (P<0,001) eram mais pesados e consumiram (diário) mais ração. Os abatidos aos 75 dias (P<0,001) ganharam mais peso (diário) e apresentaram melhor conversão alimentar. Coelhos abatidos aos 90 dias de idade tiveram maiores pesos relativos de carcaça...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coelho; Ganho de peso; Rendimento de carcaça.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600025
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Desempenho de coelhos em crescimento criados em diferentes densidades populacionais Arq. Bras. Med. Vet. Zootec.
Oliveira,M.C.; Almeida,C.V..
O objetivo deste experimento foi avaliar o desempenho de coelhos em crescimento criados em diferentes densidades populacionais. Foram utilizados 136 coelhos, não sexados, desmamados aos 35 dias de idade, em delineamento em blocos ao acaso, com quatro tratamentos e quatro repetições. As densidades avaliadas foram 11,67; 13,34; 15 e 16,67 coelhos/m², correspondentes à ocupação de sete, oito, nove e dez coelhos/gaiola. O período experimental foi de seis semanas. Os coelhos e a ração foram pesados para obtenção do peso final, ganho diário de peso, consumo diário de ração, conversão alimentar e produção de carne total ou kg de carne por m². Não houve diferença (P>0,05) entre densidade quanto ao peso final, ganho diário de peso, consumo diário de ração e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coelho; Densidade populacional; Desempenho; Crescimento.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000500012
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DNA topoisomerase inhibitors: biflavonoids from Ouratea species BJMBR
Grynberg,N.F.; Carvalho,M.G.; Velandia,J.R.; Oliveira,M.C.; Moreira,I.C.; Braz- Filho,R.; Echevarria,A..
Topoisomerase inhibitors are agents with anticancer activity. 7"-O-Methyl-agathisflavone (I) and amentoflavone (II) are biflavonoids and were isolated from the Brazilian plants Ouratea hexasperma and O. semiserrata, respectively. These biflavonoids and the acetyl derivative of II (IIa) are inhibitors of human DNA topoisomerases I at 200 µM, as demonstrated by the relaxation assay of supercoiled DNA, and only agathisflavone (I) at 200 µM also inhibited DNA topoisomerases II-alpha, as observed by decatenation and relaxation assays. The biflavonoids showed concentration-dependent growth inhibitory activities on Ehrlich carcinoma cells in 45-h culture, assayed by a tetrazolium method, with IC50 = 24 ± 1.4 µM for I, 26 ± 1.1 µM for II and 10 ± 0.7 µM for IIa....
Tipo: Info:eu-repo/semantics/other Palavras-chave: Biflavonoids; Amentoflavone; 7"-O-Methyl-agathisflavone; DNA topoisomerases; Ehrlich carcinoma; Human K562 leukemia.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700009
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Efeito de condicionadores químicos na cama de frango sobre o desempenho de frangos de corte Arq. Bras. Med. Vet. Zootec.
Ferreira,H.A.; Oliveira,M.C.; Traldi,A.B..
Estudou-se o efeito de condicionadores químicos na cama de frango sobre o desempenho de frangos de corte criados em três lotes consecutivos. Foram utilizadas 1320 aves, 440 em cada lote, em delineamento experimental em blocos ao acaso, com cinco tratamentos (Trat. 1 - cama sem tratamento; Trat. 2 - cama tratada com sulfato de alumínio; Trat. 3 - cama tratada com gesso agrícola; Trat. 4 - cama tratada com superfosfato simples e Trat. 5 - cama tratada com cal hidratada) e quatro repetições. As aves e as rações foram pesadas no início e no final do período experimental para obtenção do peso final, do consumo de ração, da conversão alimentar e da viabilidade. O uso dos condicionadores não influenciou (P>0,05) o peso final, o consumo de ração e a conversão...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cama de frango reutilizada; Condicionador químico; Desempenho; Frango de corte.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000400017
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Efeito de condicionadores químicos sobre a qualidade da cama de frango Arq. Bras. Med. Vet. Zootec.
Oliveira,M.C.; Ferreira,H.A.; Cancherini,L.C..
Avaliou-se a qualidade química da cama de frango submetida, por três lotes consecutivos de criação, a diferentes condicionadores químicos. Foram utilizadas 1320 aves, 440 por lote, em delineamento experimental em blocos ao acaso, com cinco tratamentos (Trat. 1 - cama sem tratamento; Trat. 2 - cama tratada com sulfato de alumínio; Trat. 3 - cama tratada com gesso agrícola; Trat. 4 - cama tratada com superfosfato simples e Trat. 5 - cama tratada com cal hidratada) e quatro repetições. As amostras de cama foram coletadas no 42º dia de utilização. Não houve influência dos condicionadores (P>0,05) sobre a matéria seca. O sulfato de alumínio reduziu (P<0,002) o valor de pH (7,42; 7,07 e 6,00, respectivamente no primeiro, segundo e terceiro lotes) e reduziu...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amônia; Cama de frango; Condicionador químico; Umidade.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000400016
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Effect of mannanoligosaccharides and/or enzymes on antibody titers against infectious bursal and Newcastle disease viruses Arq. Bras. Med. Vet. Zootec.
Oliveira,M.C.; Figueiredo-Lima,D.F.; Faria Filho,D.E.; Marques,R.H.; Moraes,V.M.B..
The effect of including mannanoligosaccharides (MOS) and/or enzymes in broiler diets on antibody titers against infectious bursal disease virus (IBDV) and Newcastle disease virus (NDV) was evaluated. A total of 750 broilers were distributed into a completely randomized experimental design in a factorial arrangement 2 x 2 + 1 with two levels of MOS (0 and 0.1% until 21 days and 0.05% from 22 to 42 days of age), two levels of enzymes (0 and 0.05%) and a positive control diet containing antibiotic, totaling five treatments with five replicates each. For antibody analyses, blood samples were weekly collected by jugular vein puncture in the same two birds per replicate. The first and last collections were done at 7 and 42 days of age, respectively. The...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Broiler; Additive; Humoral immune modulation; Prebiotic.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100002
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Evaluation of 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice BJMBR
Carlesso,F.N.; Fuscaldi,L.L.; Araújo,R.S.; Teixeira,C.S.; Oliveira,M.C.; Fernandes,S.O.A.; Cassali,G.D.; Reis,D.C.; Barros,A.L.B.; Cardoso,V.N..
Pancreatic adenocarcinoma is important in oncology because of its high mortality rate. Deaths may be avoided if an early diagnosis could be achieved. Several types of tumors overexpress gastrin-releasing peptide receptors (GRPr), including pancreatic cancer cells. Thus, a radiolabeled peptide derivative of gastrin-releasing peptide (GRP) may be useful as a specific imaging probe. The purpose of the present study was to evaluate the feasibility of using99mTc-HYNIC-βAla-Bombesin(7-14)as an imaging probe for Capan-1 pancreatic adenocarcinoma. Xenographic pancreatic tumor was developed in nude mice and characterized by histopathological analysis. Biodistribution studies and scintigraphic images were carried out in tumor-bearing nude mice. The two methods...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 99mTc-HYNIC-βAla-Bombesin; Capan-1; Scintigraphic images; Pancreas tumor; Biodistribution.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000010
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Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly BJMBR
Mendes,G.A.; Haag,T.; Trott,G.; Rech,C.G.S.L.; Ferreira,N.P.; Oliveira,M.C.; Kohek,M.B.; Pereira-Lima,J.F.S..
Pituitary adenomas account for 10–15% of primary intracranial tumors. Growth hormone (GH)-secreting adenomas account for 13% of all pituitary adenomas and cause acromegaly. These tumors can be aggressive, invade surrounding structures and are highly recurrent. The objective of this study was to evaluate E-cadherin, Slug and neural cell adhesion molecule (NCAM) expression in GH-secreting pituitary adenomas and its relationship to tumor invasiveness. A cross–sectional study of patients who underwent hypophysectomy due to GH-secreting pituitary adenoma from April 2007 to December 2014 was carried out. The medical records were reviewed to collect clinical data. Immediately after surgery, tumor samples were frozen in liquid nitrogen and stored in a biofreezer...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acromegaly; Pituitary neoplasms; Cadherins; Slug; Neural cell adhesion molecules.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200605
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Expression of merlin, NDRG2, ERBB2, and c-MYC in meningiomas: relationship with tumor grade and recurrence BJMBR
Ongaratti,B.R.; Silva,C.B.O.; Trott,G.; Haag,T.; Leães,C.G.S.; Ferreira,N.P.; Oliveira,M.C.; Pereira-Lima,J.F.S..
Meningiomas are common, usually benign tumors of the central nervous system that have a high rate of post-surgical recurrence or regrowth. We determined expression of the proteins merlin, NDRG2, ERBB2, and c-MYC in meningiomas using immunohistochemistry and assessed relationships between protein expression and gender, age, tumor grade, and recurrence or regrowth. The study sample comprised 60 patients, (44 women and 16 men) with a mean age of 53.2±12.7 years. Tumors were classified as grade I (n=48) or grades II and III (n=12). Expression of merlin, NDRG2, ERBB2, and c-MYC was not significantly different statistically with relation to gender, age, or meningioma recurrence or regrowth. Merlin was expressed in 100% of the cases. No statistically significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Meningioma; Merlin; NDRG2; ERBB2; C-MYC; Immunohistochemistry.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400706
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Expression of p53 protein in pituitary adenomas BJMBR
Oliveira,M.C.; Marroni,C.P.; Pizarro,C.B.; Pereira-Lima,J.F.; Barbosa-Coutinho,L.M.; Ferreira,N.P..
Inactivating mutations of TP53, a tumor suppressor gene, are associated with abnormal cell proliferation. Although p53 expression is common in many human malignancies, p53 protein has seldom been evaluated in pituitary tumors. When detected, the percentage of p53-positive cells is low, and, in general, it is exclusive for invasive lesions. The aim of the present study was to use immunohistochemistry to determine the presence of p53 protein in pituitary adenomas from tumor samples of 163 surgeries performed in 148 patients (40% male, 60% female). In 35% of the cases the adenoma was nonfunctional, while in the others it was associated with PRL, GH and/or ACTH endocrine hypersecretion syndrome. Macroadenomas were observed in 83.2% of the cases with available...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Pituitary; Pituitary adenoma; Invasive adenoma; P53; Immunohistochemistry.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500008
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Genome features of Leptospira interrogans serovar Copenhageni BJMBR
Nascimento,A.L.T.O.; Verjovski-Almeida,S.; Van Sluys,M.A.; Monteiro-Vitorello,C.B.; Camargo,L.E.A.; Digiampietri,L.A.; Harstkeerl,R.A.; Ho,P.L.; Marques,M.V.; Oliveira,M.C.; Setubal,J.C.; Haake,D.A.; Martins,E.A.L..
We report novel features of the genome sequence of Leptospira interrogans serovar Copenhageni, a highly invasive spirochete. Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in mammals. Genomic sequence analysis reveals the presence of a competent transport system with 13 families of genes encoding for major transporters including a three-member component efflux system compatible with the long-term survival of this organism. The leptospiral genome contains a broad array of genes encoding regulatory system, signal transduction and methyl-accepting chemotaxis proteins, reflecting the organism's ability to respond to diverse environmental stimuli. The identification of a complete set...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospira interrogans; Outer membrane proteins; Lipopolysaccharides; Transport; Regulatory systems.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400003
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Male gonadal function, prolactin secretion and lactotroph population in an experimental model of cirrhosis BJMBR
Gonzales,P.H.; Rhoden,C.R.; Luz,C.; Corrêa,G.; Barbosa-Coutinho,L.M.; Oliveira,M.C..
Liver cirrhosis, a highly prevalent chronic disease, is frequently associated with endocrine dysfunctions, notably in the gonadal axis. We evaluated lactotroph population by immunohistochemistry, gonadotropins and prolactin by immunoradiometric assay and testosterone and estradiol by radioimmunoassay in adult male Wistar rats with cirrhosis induced by carbon tetrachloride. No significant difference in mean ± SEM percentages of lactotrophs was found between cirrhotic animals and controls (N = 12, mean 18.95 ± 1.29%). Although there was no significant difference between groups in mean serum levels of prolactin (control: 19.2 ± 4 ng/mL), luteinizing hormone (control: 1.58 ± 0.43 ng/mL), follicle-stimulating hormone (control: 19.11 ± 2.28 ng/mL), estradiol...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hypogonadism; Hyperestrogenemia; Hyperprolactinemia; Lactotrophs; Liver cirrhosis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000011
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Measurement of Ki-67 antigen in 159 pituitary adenomas using the MIB-1 monoclonal antibody BJMBR
Pizarro,C.B.; Oliveira,M.C.; Coutinho,L.B.; Ferreira,N.P..
Pituitary adenomas sometimes show rapid growth and recurrence, and about one third invade the structures surrounding the sella turcica. In an attempt to determine aggressive behavior at an early stage, we used the MIB-1 antibody to identify the Ki-67 antigen. The present study was designed to evaluate pituitary adenomatous tissue in terms of secretion and proliferation and to correlate the Ki-67 index with hormone phenotype and invasive behavior. Material from 159 patients submitted to one or more resections of pituitary adenomas was evaluated. Forty-two non-secretory adenomas and 43 adenomas immunoreactive for growth hormone, 19 for prolactin, 18 for growth hormone and prolactin, 16 for adrenocorticotropic hormone (ACTH), and 21 cases of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pituitary adenoma; Invasive adenoma; Ki-67 antigen; MIB-1 antibody; Cell proliferation; Immunohistochemistry.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200011
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Performance and morphology of intestinal mucosa of broilers fed mannan-oligosaccharides and enzymes Arq. Bras. Med. Vet. Zootec.
Oliveira,M.C.; Rodrigues,E.A.; Marques,R.H.; Gravena,R.A.; Guandolini,G.C.; Moraes,V.M.B..
The performance and the morphology of intestinal mucosa of broilers fed mannan-oligosaccharides (MOS) and enzymes (E) from one to 21-day-old were evaluated using 750 one-day-old chicks, assigned to a 2 x 2 + 1 factorial design - two levels of MOS (0 and 0.1%), two levels of E (0 and 0.05%) plus an antibiotic positive control diet - performing five treatments of five replications each one. MOS x E interaction was significant for both duodenal (P<0.002 and P<0.002) and ileal (P<0.04 and P<0.05) perimeters and heights of villi, being the values lower in the mucosa of birds fed non-supplemented diets. MOS based-diet determined an increase on perimeter of jejunal villi (P<0.05). Compared with antibiotic treatment group, villi perimeter...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler; Nutrition; Enzymes; Prebiotic.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200025
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Pituitary glycoprotein hormone a-subunit secretion by cirrhotic patients BJMBR
Oliveira,M.C.; Pizarro,C.B.; Cassal,A.; Cremonese,R.; Vieira,J.G.H..
Secretion of the <FONT FACE="Symbol">a</font>-subunit of pituitary glycoprotein hormones usually follows the secretion of intact gonadotropins and is increased in gonadal failure and decreased in isolated gonadotropin deficiency. The aim of the present study was to determine the levels of the <FONT FACE="Symbol">a</font>-subunit in the serum of patients with cirrhosis of the liver and to compare the results obtained for eugonadal cirrhotic patients with those obtained for cirrhotic patients with hypogonadotropic hypogonadism. Forty-seven of 63 patients with cirrhosis (74.6%) presented hypogonadism (which was central in 45 cases and primary in 2), 7 were eugonadal, and 9 women were in normal menopause. The serum <FONT...
Tipo: Info:eu-repo/semantics/article Palavras-chave: <; FONT FACE=Symbol>; A<; /font>; -subunit pituitary gonadotropins hypogonadism cirrhosis.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100011
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Utilização de nutrientes em frangos alimentados com dietas suplementadas com fitase e níveis reduzidos de fósforo não-fítico Arq. Bras. Med. Vet. Zootec.
Oliveira,M.C.; Gravena,R.A.; Marques,R.H.; Guandolini,G.C.; Moraes,V.M.B..
Avaliaram-se a digestibilidade ileal, retenção de nutrientes e valores de energia metabolizável aparente (EMA) de dietas suplementadas com fitase e níveis reduzidos de fósforo não-fítico (FNF) para frangos de corte. Foram utilizados 330 pintos machos em delineamento em blocos ao acaso e esquema fatorial 2 x 3 com dois níveis de fitase (0 e 25U/kg) e três níveis de FNF (100, 85 e 70% das exigências da ave), totalizando seis tratamentos com cinco repetições de 11 aves cada. Não houve efeito da interação FNF vs fitase e dos níveis de FNF ou fitase sobre a retenção de matéria seca e de fósforo, e a interação FNF vs fitase foi significativa para retenção de proteína bruta e coeficiente de digestibilidade ileal da matéria seca. A suplementação com fitase,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Deficiência de fósforo; Enzima; Valor nutritivo.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200024
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