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| Registros recuperados: 49 | |
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| Dwivedi, P.P.; Patel, B.K.C.; Rees, G.N.; Ollivier, Bernard. |
| A method for DNA sequencing of ribosomal RNA (rRNA) genes, amplified by polymerase chain reaction (PCR), using internal primers, designed on the basis of conserved regions of rRNA genes for determining a near complete sequence (99%) of the gene using an automated DNA sequencer (Applied Biosystem Incorporation, USA) is described. The procedure is extremely rapid as cloning of the gene is not required for sequence determination. In addition time consuming steps such as ethanol precipitation and hazardous steps such as phenol/chloroform extractions are excluded from the protocol for the purification of extension products after Taq cycle sequencing using the ABI dye terminator chemistry. The method has been successfully used for sequencing of the 16S and 18S... |
| Tipo: Text |
Palavras-chave: BIOLOGIE MOLECULAIRE; GENE; ARN; METHODE D'ANALYSE; PCR.REACTION DE POLYMERISATION EN CHAINE. |
| Ano: 1996 |
URL: http://www.documentation.ird.fr/hor/fdi:010009566 |
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| Bes, Meline; Merrouch, Meriem; Joseph, Manon; Quemeneur, Marianne; Payri, Claude; Pelletier, Bernard; Ollivier, Bernard; Fardeau, Marie-laure; Erausol, Gael; Postec, Anne. |
| A novel anaerobic bacterial strain, ST07-YET, was isolated from a carbonate chimney of the Prony Hydrothermal Field (PHF) in New Caledonia. Cells were Gram-stain-positive, straight rods (0.7-0.8 x 3.0-5.0 mu m) and motile by means of lateral flagella. Strain ST07-YET was mesophilic (optimum 35 degrees C), moderately alkaliphilic and halotolerant (optimum pH 8.7 and 5 gr l(-1) NaCl). Elemental sulfur, sulfate, thiosulfate, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Yeast extract, peptone, tryptone, Casamino acids, crotonate, pyruvate, galactose, maltose, sucrose, ribose, trehalose and glucose were used as carbon sources. Glucose fermentation led to acetate, H-2 and CO2 formation. Arginine, serine, histidine, lysine,... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00467/57917/60305.pdf |
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| Brisbarre, Nadège; Fardeau, Marie-laure; Cueff, Valerie; Cayol, Jean-luc; Barbier, Georges; Cilia, Valérie; Ravot, Gilles; Thomas, Pierre; Garcia, Jean-louis; Ollivier, Bernard. |
| A strictly anaerobic, slightly halophilic and moderately thermophilic, sporulating rod designated strain DVird3(T) was isolated from deep-sea hydrothermal vent samples collected at a depth of approximately 800 m on the Atlantic Ocean Ridge. Strain DVird3(T) possessed a few laterally inserted flagella, had a DNA G+C content of 33.1 mol% and grew optimally at pH 6.6 and at 45 degreesC. Growth was observed at temperatures between 20 and 58 degreesC and at pH values between 5.8 and 8.2. The optimum NaCl concentration for growth was 3% sea salt (30 g l(-1)); no growth was observed in the presence of 15 or 60 g sea salt l(-1). Strain DVird3(T) is heterotrophic and utilizes some sugars and various single amino acids. Acetate was the main fatty acid detected from... |
| Tipo: Text |
Palavras-chave: Phylogeny; Thermophile; Bacteria; Deep sea hydrothermal vent. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-588.pdf |
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| Postec, Anne; Urios, Laurent; Lesongeur, Francoise; Ollivier, Bernard; Querellou, Joel; Godfroy, Anne. |
| The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90 degrees C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA. Additional sequences phylogenetically related to Thermococus and epsilon-Proteobacteria were detected by cloning and sequencing of 16S rDNA from two samples of the enrichment culture. In comparison, the sequences retrieved from cloning analysis from an enrichment culture... |
| Tipo: Text |
Palavras-chave: Sequence alignment; Anaerobic digester; Thermotogales; Thermophile; Deep sea vent. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-364.pdf |
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| Cayol, Jean-Luc; Ollivier, Bernard; Patel, B.K.C.; Ravot, G.; Magot, M.; Ageron, E.; Grimont, P.A.D.; Garcia, Jean-Louis. |
| A strictly anaerobic, thermophilic, gram-positive, spore-forming eubacterium designated strain SEBR 5268 was isolated from an oil field at a depth of 2,100 m, where the temperature was 92°C. The cells of this organism were gram-positive, straight, motile rods (0,5 by 2 to 3 micrometers) with peritrichous flagella. The cells occurred singly or in pairs during the logarithmic growth phase, but were pleomorphic and filamentous (length, 15 micrometers) in old cultures. Growth occurred at temperatures of 40 to 75°C, and optimum growth occurred at temperatures between 55 and 60°C. The fermentable substrates included glucose, fructose, galactose, mannose, cellobiose, maltose, sucrose, lactose, D-xylose, D-ribose, mannitol, pyruvate, and starch. The products of... |
| Tipo: Text |
Palavras-chave: FERMENTATION; BACTERIOLOGIE; BACTERIE THERMOPHILE; METABOLISME; TAXONOMIE; ADN. |
| Ano: 1995 |
URL: http://www.documentation.ird.fr/hor/fdi:010005866 |
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| Pérez-bernal, Maria Fernanda; Brito, Elcia M. S.; Bartoli, Manon; Aubé, Johanne; Ollivier, Bernard; Guyoneaud, Rémy; Hirschler-réa, Agnès. |
| A novel Gram-negative, non-spore-forming, vibrio-shaped, anaerobic, alkaliphilic, sulfate-reducing bacterium, designated strain PAR22NT, was isolated from sediment samples collected at an alkaline crater lake in Guanajuato (Mexico). Strain PAR22NT grew at temperatures between 15 and 37 °C (optimum, 32 °C), at pH between pH 8.3 and 10.1 (optimum, pH 9.0–9.6), and in the presence of NaCl up to 10 %. Pyruvate, 2-methylbutyrate and fatty acids (4–18 carbon atoms) were used as electron donors in the presence of sulfate as a terminal electron acceptor and were incompletely oxidized to acetate and CO2. Besides sulfate, both sulfite and elemental sulfur were also used as terminal electron acceptors and were reduced to sulfide. The predominant fatty acids were... |
| Tipo: Text |
Palavras-chave: Alkaliphilic anaerobic bacterium; Sulfate reduction; Desulfobotulus. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00622/73396/72613.pdf |
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| Fardeau, Marie-Laure; Faudon, C.; Cayol, Jean-Luc; Magot, M.; Patel, B.K.C.; Ollivier, Bernard. |
| #Thermoanaerobacter finnii$ fermente le glucose et le xylose en lactate, acétate, H2, CO2 et éthanol qui est le produit majeur du métabolisme. Une autre bactérie du genre #Thermoanaerobacter$ isolée d'une eau de gisement pétrolier produit des quantités aussi importantes d'éthanol que de lactate à partir de ces sucres. L'oxydation du glucose et du xylose par ces deux micro-organismes est complètement modifiée en présence de thiosulfate puisque, dans ces conditions, l'acétate devient le produit majeur du métabolisme des sucres. Cette modification du métabolisme amène à de meilleurs rendements cellulaires et à un meilleur taux de croissance bactérienne lorsque le thiosulfate est utilisé comme accepteur d'électrons. Une plus grande production d'acétate en... |
| Tipo: Text |
Palavras-chave: MICROBIOLOGIE; BACTERIE; FERMENTATION; ANAEROBIOSE; METABOLISME; OXYDATION; SUCRE; ECOSYSTEME; PETROLE; THIOSULFATE. |
| Ano: 1996 |
URL: http://www.documentation.ird.fr/hor/fdi:010004497 |
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| Ravot, G.; Ollivier, Bernard; Patel, B.K.C.; Magot, M.; Garcia, Jean-Louis. |
| We found that #Thermosipho africanus$ was able to ferment D-glucose, D-ribose, maltose, and starch, while D-galactose, fructose, and sucrose were utilized poorly. Acetate, H2, and CO2, as well as small amounts of ethanol and lactate, were end products of glucose metabolism in this organism. The presence of thiosulfate as an electron acceptor greatly improved growth and increased acetate production from the sugars. The genus #Thermosipho$ is the only genus in the order #Thermotogales$ that has been described as a non-carbohydrate fermenter. We propose that the description of the genus #Thermosipho$ be emended because the only species in this genus, #T. africanus$, is a carbohydrate fermenter that is able to utilize thiosulfate as an electron acceptor.... |
| Tipo: Text |
Palavras-chave: FERMENTATION; BACTERIOLOGIE; BACTERIE; MILIEU DE CULTURE; METABOLISME; TAXONOMIE; THIOSULFATE. |
| Ano: 1996 |
URL: http://www.documentation.ird.fr/hor/fdi:010005868 |
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| Registros recuperados: 49 | |
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