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Registros recuperados: 49
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A rapid method for sequencing of rRNA gene(s) amplified by polymerase chain reaction using an automated DNA sequencer IRD
Dwivedi, P.P.; Patel, B.K.C.; Rees, G.N.; Ollivier, Bernard.
A method for DNA sequencing of ribosomal RNA (rRNA) genes, amplified by polymerase chain reaction (PCR), using internal primers, designed on the basis of conserved regions of rRNA genes for determining a near complete sequence (99%) of the gene using an automated DNA sequencer (Applied Biosystem Incorporation, USA) is described. The procedure is extremely rapid as cloning of the gene is not required for sequence determination. In addition time consuming steps such as ethanol precipitation and hazardous steps such as phenol/chloroform extractions are excluded from the protocol for the purification of extension products after Taq cycle sequencing using the ABI dye terminator chemistry. The method has been successfully used for sequencing of the 16S and 18S...
Tipo: Text Palavras-chave: BIOLOGIE MOLECULAIRE; GENE; ARN; METHODE D'ANALYSE; PCR.REACTION DE POLYMERISATION EN CHAINE.
Ano: 1996 URL: http://www.documentation.ird.fr/hor/fdi:010009566
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Acetoanaerobium pronyense sp nov., an anaerobic alkaliphilic bacterium isolated from a carbonate chimney of the Prony Hydrothermal Field (New Caledonia) ArchiMer
Bes, Meline; Merrouch, Meriem; Joseph, Manon; Quemeneur, Marianne; Payri, Claude; Pelletier, Bernard; Ollivier, Bernard; Fardeau, Marie-laure; Erausol, Gael; Postec, Anne.
A novel anaerobic bacterial strain, ST07-YET, was isolated from a carbonate chimney of the Prony Hydrothermal Field (PHF) in New Caledonia. Cells were Gram-stain-positive, straight rods (0.7-0.8 x 3.0-5.0 mu m) and motile by means of lateral flagella. Strain ST07-YET was mesophilic (optimum 35 degrees C), moderately alkaliphilic and halotolerant (optimum pH 8.7 and 5 gr l(-1) NaCl). Elemental sulfur, sulfate, thiosulfate, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Yeast extract, peptone, tryptone, Casamino acids, crotonate, pyruvate, galactose, maltose, sucrose, ribose, trehalose and glucose were used as carbon sources. Glucose fermentation led to acetate, H-2 and CO2 formation. Arginine, serine, histidine, lysine,...
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Ano: 2015 URL: https://archimer.ifremer.fr/doc/00467/57917/60305.pdf
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Aislamiento y caracterizacion de bacterias tiosulfato reductoras no sulfato reductoras en digestores anaerobios mesofilicos IRD
Hernandez, G.; Fardeau, Marie-Laure; Patel, B.K.C.; Macarie, Hervé; Garcia, Jean-Louis; Ollivier, Bernard.
Tipo: Text Palavras-chave: BACTERIE MESOPHILE; ANAEROBIOSE; SOUFRE; SULFATOREDUCTION; CHROMATOGRAPHIE EN PHASE LIQUIDE; TECHNIQUE PCR; EAU USEE; ETUDE EXPERIMENTALE; ETUDE COMPARATIVE; TIOSULFATOREDUCTION.
Ano: 1998 URL: http://www.documentation.ird.fr/hor/fdi:010021432
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Anaerobic bacteria from hypersaline environments IRD
Ollivier, Bernard; Caumette, P.; Garcia, Jean-Louis; Mah, R.A..
Tipo: Text Palavras-chave: BACTERIE; ANAEROBIOSE; FERMENTATION; METHANOGENESE; SULFATOREDUCTION; TAXONOMIE; ECOLOGIE; EAU CONTINENTALE; HYPERSALINITE; BACTERIE PHOTOTROPHE; BACTERIE SULFATO REDUCTRICE; BACTERIE FERMENTAIRE; BACTERIE ANAEROBIE; BACTERIE METHANOGENE; SYNTHESE BIBLIOGRAPHIQUE.
Ano: 1994 URL: http://www.documentation.ird.fr/hor/fdi:41111
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Bacillus thermoamylovorans sp. nov., a moderatly thermophilic and amylolytic bacterium IRD
Combet-Blanc, Yannick; Ollivier, Bernard; Streicher, C.; Patel, B.K.C.; Dwivedi, P.; Pot, B.; Prensier, G.; Garcia, Jean-Louis.
Tipo: Text Palavras-chave: MICROBIOLOGIE; BACTERIE; FERMENTATION; ANAEROBIOSE; THERMOPHILIE; ESPECE NOUVELLE; PHYLOGENIE; LACTATE; AMYLOLYSE; VIN DE PALME.
Ano: 1995 URL: http://www.documentation.ird.fr/hor/fdi:41838
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Characterization of Desulfovibrio fructosovorans sp. nov. IRD
Ollivier, Bernard; Cord-Ruwisch, Ralf; Hatchikian, E.C.; Garcia, Jean-Louis.
Tipo: Text Palavras-chave: BACTERIE; ANAEROBIOSE; MILIEU DE CULTURE; ESPECE NOUVELLE; DESCRIPTION; SULFATOREDUCTION; FERMENTATION; FRUCTOSE; METHODOLOGIE; ESTUAIRE; SEDIMENT.
Ano: 1988 URL: http://www.documentation.ird.fr/hor/fdi:26564
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Clostridium caminithermale sp nov., a slightly halophilic and moderately thermophilic bacterium isolated from an Atlantic deep-sea hydrothermal chimney ArchiMer
Brisbarre, Nadège; Fardeau, Marie-laure; Cueff, Valerie; Cayol, Jean-luc; Barbier, Georges; Cilia, Valérie; Ravot, Gilles; Thomas, Pierre; Garcia, Jean-louis; Ollivier, Bernard.
A strictly anaerobic, slightly halophilic and moderately thermophilic, sporulating rod designated strain DVird3(T) was isolated from deep-sea hydrothermal vent samples collected at a depth of approximately 800 m on the Atlantic Ocean Ridge. Strain DVird3(T) possessed a few laterally inserted flagella, had a DNA G+C content of 33.1 mol% and grew optimally at pH 6.6 and at 45 degreesC. Growth was observed at temperatures between 20 and 58 degreesC and at pH values between 5.8 and 8.2. The optimum NaCl concentration for growth was 3% sea salt (30 g l(-1)); no growth was observed in the presence of 15 or 60 g sea salt l(-1). Strain DVird3(T) is heterotrophic and utilizes some sugars and various single amino acids. Acetate was the main fatty acid detected from...
Tipo: Text Palavras-chave: Phylogeny; Thermophile; Bacteria; Deep sea hydrothermal vent.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-588.pdf
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Clostridium thermopalmarium sp. nov.; a moderately thermophilic butyrate-producing bacterium isolated from palm wine in Senegal IRD
Lawson Anani Soh, A.; Ralambotiana, H.; Ollivier, Bernard; Prensier, G.; Tine, E.; Garcia, Jean-Louis.
Tipo: Text Palavras-chave: BIOTECHNOLOGIE; BACTERIE; ANAEROBIOSE; TAXONOMIE; THERMOPHILIE; ESPECE NOUVELLE; ISOLEMENT; METHODE D'ANALYSE; VIN DE PALME; BUTYRATE.
Ano: 1991 URL: http://www.documentation.ird.fr/hor/fdi:34554
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Continuous enrichment culture and molecular monitoring to investigate the microbial diversity of thermophiles inhabiting deep-sea hydrothermal ecosystems ArchiMer
Postec, Anne; Urios, Laurent; Lesongeur, Francoise; Ollivier, Bernard; Querellou, Joel; Godfroy, Anne.
The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90 degrees C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA. Additional sequences phylogenetically related to Thermococus and epsilon-Proteobacteria were detected by cloning and sequencing of 16S rDNA from two samples of the enrichment culture. In comparison, the sequences retrieved from cloning analysis from an enrichment culture...
Tipo: Text Palavras-chave: Sequence alignment; Anaerobic digester; Thermotogales; Thermophile; Deep sea vent.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-364.pdf
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Description of Thermoanaerobacter brockii subsp. lactiethylicus subsp. nov. isolated from a deep subsurface french oil well : a proposal to reclassify Thermoanaerobacter finnii as Thermoanaerobacter brockii subsp. finnii comb. nov. and an emended description of Thermoanaerobacter brockii IRD
Cayol, Jean-Luc; Ollivier, Bernard; Patel, B.K.C.; Ravot, G.; Magot, M.; Ageron, E.; Grimont, P.A.D.; Garcia, Jean-Louis.
A strictly anaerobic, thermophilic, gram-positive, spore-forming eubacterium designated strain SEBR 5268 was isolated from an oil field at a depth of 2,100 m, where the temperature was 92°C. The cells of this organism were gram-positive, straight, motile rods (0,5 by 2 to 3 micrometers) with peritrichous flagella. The cells occurred singly or in pairs during the logarithmic growth phase, but were pleomorphic and filamentous (length, 15 micrometers) in old cultures. Growth occurred at temperatures of 40 to 75°C, and optimum growth occurred at temperatures between 55 and 60°C. The fermentable substrates included glucose, fructose, galactose, mannose, cellobiose, maltose, sucrose, lactose, D-xylose, D-ribose, mannitol, pyruvate, and starch. The products of...
Tipo: Text Palavras-chave: FERMENTATION; BACTERIOLOGIE; BACTERIE THERMOPHILE; METABOLISME; TAXONOMIE; ADN.
Ano: 1995 URL: http://www.documentation.ird.fr/hor/fdi:010005866
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Desulfobotulus mexicanus sp. nov., a novel sulfate-reducing bacterium isolated from the sediment of an alkaline crater lake in Mexico ArchiMer
Pérez-bernal, Maria Fernanda; Brito, Elcia M. S.; Bartoli, Manon; Aubé, Johanne; Ollivier, Bernard; Guyoneaud, Rémy; Hirschler-réa, Agnès.
A novel Gram-negative, non-spore-forming, vibrio-shaped, anaerobic, alkaliphilic, sulfate-reducing bacterium, designated strain PAR22NT, was isolated from sediment samples collected at an alkaline crater lake in Guanajuato (Mexico). Strain PAR22NT grew at temperatures between 15 and 37 °C (optimum, 32 °C), at pH between pH 8.3 and 10.1 (optimum, pH 9.0–9.6), and in the presence of NaCl up to 10 %. Pyruvate, 2-methylbutyrate and fatty acids (4–18 carbon atoms) were used as electron donors in the presence of sulfate as a terminal electron acceptor and were incompletely oxidized to acetate and CO2. Besides sulfate, both sulfite and elemental sulfur were also used as terminal electron acceptors and were reduced to sulfide. The predominant fatty acids were...
Tipo: Text Palavras-chave: Alkaliphilic anaerobic bacterium; Sulfate reduction; Desulfobotulus.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00622/73396/72613.pdf
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Desulfohalobium retbaense gen. nov., sp. nov., a halophilic sulfate-reducing bacterium from sediments of a hypersaline lake in Senegal IRD
Ollivier, Bernard; Hatchikian, C.E.; Prensier, G.; Guezennec, J.; Garcia, Jean-Louis.
Tipo: Text Palavras-chave: BIOTECHNOLOGIE; BACTERIE; SULFATOREDUCTION; GENRE NOUVEAU; ESPECE NOUVELLE; TAXONOMIE; ISOLEMENT; SEDIMENT; LAC SALE; HALOPHILE.
Ano: 1991 URL: http://www.documentation.ird.fr/hor/fdi:34555
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Determination of the G + C content of two Syntrophus buswellii strains by ultracentrifugation techniques IRD
Fardeau, Marie-Laure; Ollivier, Bernard; Soubrane, Anne; Sauve, P.; Prensier, G.; Garcia, Jean-Louis; Bélaich, J.P..
Tipo: Text Palavras-chave: MICROBIOLOGIE; BACTERIE; ANAEROBIOSE; METABOLISME; ADN; METHODE D'ANALYSE; COMPOSITION CHIMIQUE; BENZOATE; ULTRACENTRIFUGATION.
Ano: 1993 URL: http://www.documentation.ird.fr/hor/fdi:41110
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Ecologie microbienne de la digestion anaérobie : techniques de numérotation et d'isolement IRD
Garcia, Jean-Louis; Guyot, Jean-Pierre; Ollivier, Bernard; Trad, Monica; Paycheng, Claude.
Tipo: Text Palavras-chave: MICROFLORE; DIGESTION; ANAEROBIOSE; NUMERATION; ISOLEMENT; TECHNIQUE; BACILLUS; CLOSTRIDIUM.
Ano: 1982 URL: http://www.documentation.ird.fr/hor/fdi:03179
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Effect of thiosulphate as electron acceptor on glucose and xylose oxidation by Thermoanaerobacter finnii and Thermoanaerobacter sp. isolated from oil field water IRD
Fardeau, Marie-Laure; Faudon, C.; Cayol, Jean-Luc; Magot, M.; Patel, B.K.C.; Ollivier, Bernard.
#Thermoanaerobacter finnii$ fermente le glucose et le xylose en lactate, acétate, H2, CO2 et éthanol qui est le produit majeur du métabolisme. Une autre bactérie du genre #Thermoanaerobacter$ isolée d'une eau de gisement pétrolier produit des quantités aussi importantes d'éthanol que de lactate à partir de ces sucres. L'oxydation du glucose et du xylose par ces deux micro-organismes est complètement modifiée en présence de thiosulfate puisque, dans ces conditions, l'acétate devient le produit majeur du métabolisme des sucres. Cette modification du métabolisme amène à de meilleurs rendements cellulaires et à un meilleur taux de croissance bactérienne lorsque le thiosulfate est utilisé comme accepteur d'électrons. Une plus grande production d'acétate en...
Tipo: Text Palavras-chave: MICROBIOLOGIE; BACTERIE; FERMENTATION; ANAEROBIOSE; METABOLISME; OXYDATION; SUCRE; ECOSYSTEME; PETROLE; THIOSULFATE.
Ano: 1996 URL: http://www.documentation.ird.fr/hor/fdi:010004497
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Emendation of the genus Thermobacteroides: Thermobacteroides proteolyticus sp. nov., a proteolytic acetogen from a methanogenic enrichment IRD
Ollivier, Bernard; Mah, R.A.; Ferguson, T.J.; Boone, D.R.; Garcia, Jean-Louis; Robinson, R..
Tipo: Text Palavras-chave: MICROBIOLOGIE; TAXONOMIE; BACTERIE; ESPECE NOUVELLE; MILIEU DE CULTURE; ANAEROBIOSE; THERMOPHILIE; METHANOGENESE; METHODOLOGIE; PROTEOLYSE; REVISION TAXONOMIQUE; CARACTERISATION; ACETOGENESE.
Ano: 1985 URL: http://www.documentation.ird.fr/hor/fdi:34000
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Emended description of Thermosipho africanus as a carbohydrate- fermenting species using thiosulfate as an electron acceptor IRD
Ravot, G.; Ollivier, Bernard; Patel, B.K.C.; Magot, M.; Garcia, Jean-Louis.
We found that #Thermosipho africanus$ was able to ferment D-glucose, D-ribose, maltose, and starch, while D-galactose, fructose, and sucrose were utilized poorly. Acetate, H2, and CO2, as well as small amounts of ethanol and lactate, were end products of glucose metabolism in this organism. The presence of thiosulfate as an electron acceptor greatly improved growth and increased acetate production from the sugars. The genus #Thermosipho$ is the only genus in the order #Thermotogales$ that has been described as a non-carbohydrate fermenter. We propose that the description of the genus #Thermosipho$ be emended because the only species in this genus, #T. africanus$, is a carbohydrate fermenter that is able to utilize thiosulfate as an electron acceptor....
Tipo: Text Palavras-chave: FERMENTATION; BACTERIOLOGIE; BACTERIE; MILIEU DE CULTURE; METABOLISME; TAXONOMIE; THIOSULFATE.
Ano: 1996 URL: http://www.documentation.ird.fr/hor/fdi:010005868
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Fermentation méthanique par des cultures mixtes définies de bactéries thermophiles IRD
Ollivier, Bernard.
Ce travail décrit l'étude de la biométhanisation d'un résidu solide d'industrie agroalimentaire, les pulpes de betterave sucrière, par une culture mixte de bactéries thermophiles. Ces pulpes sont composées en grande partie de cellulose, de protéines et de pectines. La première phase a permis l'isolement de bactéries acidifiantes et méthanogènes thermophiles dont certaines ont été associées dans des cultures mixtes définies. Une bactérie protéolytique, #Thermobacteroides proteolyticus$, et trois bactéries méthanogènes appartenant aux genres #Methanobacterium$, #Methanogenium$ et #Methanosarcina$ ont été identifiées. Dans une deuxième étape, une expérimentation en cultures mixtes définies a été réalisée pour méthaniser la gélatine en associant...
Tipo: Text Palavras-chave: FERMENTATION METHANIQUE; THERMOPHILIE; CELLULOSE; PULPE; BETTERAVE SUCRIERE; DECHET AGROINDUSTRIEL; MICROBIOLOGIE; TAXONOMIE; COCULTURE DEFINIE; GELATINE; PECTINE; BACTERIE METHANOGENE.
Ano: 1987 URL: http://www.documentation.ird.fr/hor/fdi:24783
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Fructose degradation by Desulfovibrio sp. in pure culture and in coculture with Methanospirillum hungatei IRD
Cord-Ruwisch, R.; Ollivier, Bernard; Garcia, Jean-Louis.
Tipo: Text Palavras-chave: FERMENTATION; COCULTURE; FRUCTOSE; DEGRADATION; BACTERIE; SULFATOREDUCTION; METHODE D'ANALYSE; SPECTROMETRIE; CHROMATOGRAPHIE EN PHASE LIQUIDE; ETUDE EXPERIMENTALE.
Ano: 1986 URL: http://www.documentation.ird.fr/hor/fdi:24199
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H2 oxidation in the presence of thiosulfate, by a Thermoanaerobacter strain isolated from an oil-producing well IRD
Fardeau, Marie-Laure; Cayol, Jean-Luc; Magot, M.; Ollivier, Bernard.
Tipo: Text Palavras-chave: BACTERIE; ANAEROBIOSE; THERMOPHILIE; HYDROGENE; OXYDATION; PUITS; PETROLE; THIOSULFATE.
Ano: 1993 URL: http://www.documentation.ird.fr/hor/fdi:41109
Registros recuperados: 49
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