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Growth and sporulation of Metarhizium flavoviride var. Flavoviride on culture media and lighting regimes Scientia Agricola
Onofre,Sideney Becker; Miniuk,Cindia Mara; Barros,Neiva Monteiro de; Azevedo,João Lúcio.
Entomopathogenic fungi from the genus Metarhizium are largely used for the biological control of agricultural pests by conidia spreading on the field. Although conidia production is well studied in M. anisopliae, only few research studies were done in M. flavoviride. The present work was carried out alming to evaluate the Mycelial growth and sporulation of the entomopathogenic fungus Metarhizium flavoviride var. flavoviride growing at 27 ± 2°C on Potato-dextrose-agar (PDA), Czapek-agar (CZP) and a complete agar medium (CM) under three lighting regimes, (continuous illumination, light/dark cycle and an black light/dark cycle) were investigated. A completely randomized 3 × 3 (culture media × lighting regime) factorial design with four replicates was used....
Tipo: Info:eu-repo/semantics/report Palavras-chave: Metarhizium flavoviride; Mycelial growth; Culture media; Lighting regimes.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000300026
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LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae) BABT
Onofre,Sideney Becker; Riveros Gonzalez,Raul; Messias,Cláudio Luiz; Azevedo,João Lúcio; Barros,Neiva Monteiro de.
The entomopathogenic fungus Nomuraea rileyi (Farlow) Samson produced a peptide active against Anticarsia gemmatalis 3rd instar larvae. To produce this peptide, N. rileyi was cultivated aerobically in Saboraud, maltose, yeast-extract broth at 26 ± 1ºC for 12 days, after which the medium was filtered and separated in a liquid/liquid extractor, concentrated and the peptide purified chromatographically. The crystals obtained were kept refrigerated until needed for LC50 analysis. The LC50 of this peptide against A. gemmatalis 3rd instar larvae was determined in triplicate experiments using solutions containing 1.0, 0.2, 0.1, 0.01, 0.001 and 0.0001 mg/ml of N. rileyi peptide. The results of these experiments were used to calculate a linear equation in which Y =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticarsia gemmatalis; Nomuraea rileyi; Integrated control; Secondary metabolite; Toxins.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300004
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