|
|
|
| Registros recuperados: 21 | |
|
| |
|
|
| PEREIRA, W. J.; BASSINELLO, P. Z.; BRONDANI, C.; VIANELLO, R. P.. |
| An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory routine for gene expression analysis. In this study, an extraction protocol for RNA from common bean seeds, which produced material with good integrity for qPCR (RIN ≥ 6.5), was optimized. In addition, 10 reference genes were evaluated under qPCR standard conditions using different tissue samples of common beans. Gene stabilities were analyzed using the delta-CT method, Bestkeeper, NormFinder and geNorm approaches. The genes β-tubulin and T197 were ranked as the most stable among the sample sets... |
| Tipo: Artigo de periódico |
Palavras-chave: Gene normalization; Molecular breeding; Grain RNA extraction; Feijão; Phaseolus vulgaris; Melhoramento; RNA; Beans; Gene expression.. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076282 |
| |
|
| |
|
|
| VALDISSER, P. A. M. R.; PEREIRA, W. J.; MORAIS JUNIOR, O. P. de; MENDONÇA, J. A.; SARTORI, D. E. L.; LIMA, LUANN V. V. O.; BORBA, T. C. de O.; BRONDANI, C.; ZUCCHI, M. I.; VIANELLO, R. P.. |
| O objetivo deste estudo foi identificar locos genéticos associados à tolerância à seca através da análise de associação genômica ampla (GWAS). |
| Tipo: Parte de livro |
Palavras-chave: Feijão; Resistência a seca; Deficiencia hídrica; Phaseolus vulgaris. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1078828 |
| |
|
| |
|
|
| SIQUEIRA, B. S.; BASSINELLO, P. Z.; MALGARESI, G.; PEREIRA, W. J.; FERNANDES, K. F.. |
| Carioca beans are subjected to the hard-to-cook phenomena (HTC), which results in loss of technological and sensorial qualities. To better elucidate this postharvest hardening process, changes in technological and biochemical properties of grains during storage at 40 °C and 75% relative humidity were investigated, and the results were analyzed using Principal Component Analysis (PCA). Results showed that the hardening process occurs at different intensity for each genotype, and those more susceptible to HTC presented higher water absorption index (WAI) and peroxidase activity. During the storage period, oxidoreductases remained active and total phenol content increased for all genotypes. PCA explained 92.2% of total variance and grouped hardness, cooking... |
| Tipo: Artigo de periódico |
Palavras-chave: Hard-to-cook; Polifenol oxidase; Feijão; Phaseolus vulgaris; Genótipo; Cotiledone; Beans; Oxidoreductases; Polyphenols; Water uptake; Cotyledons; Sensory evaluation. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1049758 |
| |
|
| |
|
| |
|
|
| PEREIRA, W. J.; SIQUEIRA, B. dos S.; DEUS, K. E. de; FERNANDES, K. F.; BASSINELLO, P. Z.; VIANELLO, R. P.. |
| O fenômeno hard-to-cook promove a diminuição no amaciamento por cocção, alteração na palatabilidade e diminuição na disponibilidade de proteínas e carboidratos, prejudicando a comercialização dos grãos. Como parte de um estudo que prevê a caracterização físico-química, bioquímicas e moleculares associadas ao endurecimento dos grãos de feijão, este estudo objetiva ajustar e implementar protocolos que possibilitem a avaliação da expressão de genes alvos responsáveis pelo endurecimento. |
| Tipo: Parte de livro |
Palavras-chave: Feijão; Phaseolus vulgaris; Cocção. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/941519 |
| |
|
|
| MÜLLER, B. S. de F.; SAKAMOTO, T.; SILVEIRA, R. D. D.; ZAMBUSSI-CARVALHO, P. F.; PEREIRA, M.; PAPPAS JUNIOR, G. J.; COSTA, M. M. do C.; GUIMARÃES, C. M.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO-BRONDANI, R. P.. |
| Drought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed... |
| Tipo: Separatas |
Palavras-chave: Feijão; Phaseolus vulgaris; Deficiência hídrica; Beans; Abiotic stress; Water stress. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/994817 |
| |
|
| |
|
|
| OLIVEIRA, J. A. V. de; ABREU, F. R. M.; PEREIRA, W. J.; SILVEIRA, R. D. D.; MENDONÇA, J. A.; VIANELLO, R. P.; BRONDANI, C.. |
| O objetivo desse trabalho foi quantificar a expressão de genes de arroz homólogos de Arabidopsis previamente relacionados ao aumento do potencial produtivo (RUBIRICE, RUBICORN, AVP1, DA1 e TOR) por meio da análise de PCR quantitativo (qPCR). Os genótipos estudados nesse estudo foram BRSMG Curinga, Primavera e Douradão, os quais foram avaliados em um ensaio em casa de vegetação, cultivados sob dois níveis de fertilidade do solo (alta e baixa), em esquema fatorial, no delineamento inteiramente casualizado e com duas repetições. |
| Tipo: Parte de livro |
Palavras-chave: Arroz; Oryza sativa; Gene; Arabidopsis.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1039417 |
| |
|
|
| ABREU, F. R. M.; DEUS, K. E. de; PEREIRA, W. J.; SILVEIRA, R. D. D.; VIANELLO, R. P.; BRONDANI, C.. |
| The identification and validation of candidate genes related to traits of interest is a time consuming and expensive process and the homology among genes from different species can facilitate the identification of genes of the target species from the genomic information of a model species. This study aimed to quantify the expression of homologous rice genes previously related to drought tolerance in Arabidopsis. Five genes (CPK6, PLDa, GluR2, CesA8, and EIN2) were identified in rice by the homology of the amino acid sequence between rice and Arabidopsis. The genotypes Douradão (drought tolerant) and Primavera (drought susceptible) were subjected to a water deficit experiment, and subsequently evaluated for gene expression by qPCR for the five homologous... |
| Tipo: Artigo de periódico |
Palavras-chave: Gene homology; Arroz; Oryza sativa; Acido abscisico; Rendimento; Rice; Abscisic acid; Quantitative polymerase chain reaction; Yields; Arabidopsis. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1054691 |
| |
|
|
| PEREIRA, W. J.; MELO, A. T. de O.; COELHO, A. S. G.; RODRIGUES, F. A.; MAMIDI, S.; ALENCAR, S. A. de; LANNA, A. C.; VALDISSER, P. A. M. R.; BRONDANI, C.; NASCIMENTO JUNIOR, I. R. do; BORBA, T. C. de O.; VIANELLO, R. P.. |
| Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs... |
| Tipo: Artigo de periódico |
Palavras-chave: Functional genomics; Feijão; Phaseolus Vulgaris; Deficiência Hídrica; Raiz; Folha; Beans; Soil water deficit; Genomics; Single nucleotide polymorphism. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121822 |
| |
|
|
| VALDISSER, P. A. M. R.; PEREIRA, W. J.; ALMEIDA FILHO, J. E.; MÜLLER, B. S. F.; COELHO, G. R. C.; MENEZES, I. P. P. de; VIANNA, J. P. G.; ZUCCHI, M. I.; LANNA, A. C.; COELHO, A. S. G.; OLIVEIRA, J. P. de; MORAES, A. da C.; BRONDANI, C.; VIANELLO, R. P.. |
| Background: Common bean is a legume of social and nutritional importance as a food crop, cultivated worldwide especially in developing countries, accounting for an important source of income for small farmers. The availability of the complete sequences of the two common bean genomes has dramatically accelerated and has enabled new experimental strategies to be applied for genetic research. DArTseq has been widely used as a method of SNP genotyping allowing comprehensive genome coverage with genetic applications in common bean breeding programs. Results: Using this technology, 6286 SNPs (1 SNP/86.5 Kbp) were genotyped in genic (43.3%) and non-genic regions (56. 7%). Genetic subdivision associated to the common bean gene pools (K = 2) and related to grain... |
| Tipo: Artigo de periódico |
Palavras-chave: Diversity arrays technology; Diversity analysis; Loci under selection; Core collection; Feijão; Phaseolus vulgaris; Genética vegetal; Linkage disequilibrium; Genotyping; Single nucleotide polymorphism. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076278 |
| |
|
|
| SIQUEIRA, B.; PEREIRA, W. J.; BATISTA, K. A.; OOMAH, B. D.; FERNANDES, K. F.; BASSINELLO, P. Z.. |
| New carioca bean cultivars are being introduced into the market necessitating their evaluation under trade conditions, which often require storage under ambient conditions. We therefore evaluated the darkening and hardening processes of six carioca bean genotypes each representing regular and slow darkening trait during storage under ambient conditions for five months to elucidate their relationship as a breeding strategy. Storage time adversely affected color characteristics (L*, a*, b*, C* and ?E) depending on bean genotype, whereas hardness and resistance to cooking increased during storage independent of the lignification process. Bean darkening and hardening occurred during storage at different intensities in each genotype and were not always... |
| Tipo: Artigo de periódico |
Palavras-chave: Feijão; Phaseolus vulgaris; Genótipo; Cocção; Hardness; Lignification; Cooking; Dry beans; Storage; Genotype. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/991440 |
| |
|
|
| DEUS, K. E. de; LANNA, A. C.; ABREU, F. R. M.; SILVEIRA, R. D. D.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO, R. P.. |
| Drought is a major cause of reduced yield in upland rice farming in many regions of the world and cause an increased production of reactive oxygen species (ROS), followed by activation of a complex antioxidant system. In this study, the superoxide dismutase (SOD) enzymatic activity and the gene expression levels of eight isoforms of SOD were evaluated in drought-tolerant and drought-sensitive varieties of upland rice, Oryza sativa japonica (the Primavera and Douradão cultivars), including shoot and root tissues in two stages of plant development, grown under normal and restricted irrigation. The SOD activity was determined by spectrophotometric method and the gene expression by qPCR experiments. In the tolerant cultivar, SOD enzymatic activity increased... |
| Tipo: Artigo de periódico |
Palavras-chave: Enzymatic activity; Arroz; Oryza sativa; Resistencia a seca; Rice; Water stress; Oxidative stress; Gene expression; Superoxide dismutase. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1037521 |
| |
|
| |
|
|
| DEUS, K. E. de; PEREIRA, W. J.; MITSUZONO, S. T.; VIANELLO, R. P.; LANNA, A. C.. |
| O estudo sobre os principais mecanismos bioquímico-moleculares utilizados pelas plantas para responder a esse tipo de estresse abiótico, se torna, portanto, essencial. Objetivou-se avaliar o sistema de defesa antioxidativo, um dos eventos metabólicos ativado sob condição de estresse, por meio da determinação da atividade da superóxido dismutase (SOD ? EC 1.15.1.1) e da catalase (CAT ? EC.1.11.1.6). |
| Tipo: Parte de livro |
Palavras-chave: Arroz; Oryza sativa; Deficiência hídrica. |
| Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/989142 |
| |
|
|
| PEREIRA, W. J.; ABREU, F. R. M.; MELO, A. T. de O.; COELHO, A. S. G.; RODRIGUES, F. A.; MAMIDI, S.; ALENCAR, S. A. de; LANNA, A. C.; VALDISSER, P. A. M. R.; BRONDANI, C.; NASCIMENTO JUNIOR, I. R. do; BORBA, T. C. de O.; VIANELLO, R. P.. |
| Este estudo foi realizado com o objetivo de promover uma ampla caracterização de genes do feijoeiro comum relacionados à seca. |
| Tipo: Parte de livro |
Palavras-chave: SNP; RNA-seq; Feijão; Phaseolus vulgaris; Resistência a seca; Beans. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1078772 |
| |
| Registros recuperados: 21 | |
|
|
|
