|
|
|
|
|
FAZA, A. P.; PINTO, I. S. B.; FONSECA, I.; ANTUNES, G. R.; MONTEIRO, C. M. DE O.; DAEMON, E.; MUNIZ, M. de S.; MARTINS, M. F.; FURLONG, J.; PRATA, M. C. de A.. |
The monitoring of resistance of cattle tick populations in Brazil to the chemical bases in use is largely limited to investigation of the phenotypic profile. There are few studies investigating the role played by the genotypic profile in acaricide resistance in the country. Therefore, the aim of the present study was to carry out molecular characterization and trace out the genetic profile of populations of Rhipicephalus microplus with respect to resistance to the organophosphate and pyrethroid chemical groups. For that purpose, larvae were genotyped belonging to 587 populations for pyrethroids and 306 for organophosphates, using the polymerase chain reaction technique. It was found that 75.49% and 97.44% of the larvae studied showed resistance to the... |
Tipo: Artigo de periódico |
Palavras-chave: Carrapato de boi; Piretroide; Genotipico. |
Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/963149 |
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
|
PINTO, I. S. B.; FONSECA, I.; BRANDAO, H. de M.; GERN, J. C.; GUIMARAES, A. S.; CARVALHO, W. A.; BRITO, M. A. V. P. e; VICCINI, L. F.; MARTINS, M. F.. |
Abstract Intramammary infections are one of the main causes of productivity loss in dairy cows. To better understand the immune system response and to avoid the use of live animals, we validated the use of isolated bovine udder as an ex situ model. Six mammary glands were collected from cows ready for culling. Three udders were perfused with Tyrode's solution and three were not-perfused. During six hours, we collected perfusate samples for biochemical analysis. We also collected alveolar and teat canal tissue to evaluate gene expression. The biochemical parameters indicated that the perfused udders remained viable for the entire period of the experiment. A real-time polymerase chain reaction showed an increase in 18S rRNA gene expression in the alveolar... |
Tipo: Separatas |
Palavras-chave: Ex-situ model; Mammary gland.; Genes.. |
Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080581 |
| |
|
| |
|
|
|