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Biological evaluation of a nutritional supplement prepared with QPM Maize cultivar BR 473 and other traditional food items BABT
Paula,Heberth de; Santos,Rinaldo C.; Silva,Marcelo E.; Glória,Enara C. S.; Pedrosa,Maria Lúcia; Almeida,Nízia A. V.; Costa,Alexandre S. V.; Malaquias,Luiz Cosme C..
Quality Protein Maize (QPM) cultivar BR 473 was employed, together with soybean flour, brown sugar, banana meal and oat meal, for the preparation of a nutritional supplement.. 21-day old male Fisher rats were fed diets containing the supplement as a protein source, both with and without soybean flour; casein diets with 10 or 7% protein served as respective controls. Protein Efficiency Ratio (PER), Net Protein Utilization (NPU), Net Protein Retention (NPR) and Digestibility were determined. Blood biochemical parameters (glucose, cholesterol, urea, hemoglobin, albumin and total protein) were also measured in the animals and showed that all were in good health condition at the end of the experiment. The obtained results for PER, NPU and NPR indicated that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: QPM Maize; Protein; Biological evaluation; Nutritional supplement.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200012
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Dietary models for inducing hypercholesterolemia in rats BABT
Matos,Sheyla Leite; Paula,Heberth de; Pedrosa,Maria Lúcia; Santos,Rinaldo Cardoso dos; Oliveira,Eduardo Luiz de; Chianca Júnior,Deoclécio Alves; Silva,Marcelo Eustáquio.
The present work aimed at finding a dietetical model capable of promoting the highest hypercholesterolemia without affecting the development of the rats. Sixty female Fisher rats were divided into five groups. The first one was fed a control diet; the remaining four were fed hypercholesterolemic diets with cholesterol and different contents of soybean oil, starch, casein, micronutrients and fiber and, consequently, different caloric values. After eight weeks animals were evaluated in relation to growth, fecal excretion, liver weight and fat, cholesterol and its fractions, serum biochemical parameters and sistolic pressure and compared with controls. The best result was obtained with the diet containing 25 % soybean oil, 1.0 % cholesterol, 13 % fiber and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hypercholesterolemia; Animal model; Caloric value; Cholesterol.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200006
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Effect of an aqueous extract of annatto (Bixa orellana) seeds on lipid profile and biochemical markers of renal and hepatic function in hipercholesterolemic rats BABT
Paula,Heberth de; Pedrosa,Maria Lúcia; Rossoni Júnior,Joamyr Victor; Haraguchi,Fabiano Kenji; Santos,Rinaldo Cardoso dos; Silva,Marcelo Eustáquio.
Annatto extract is a natural food color obtained from the outer coatings of the seeds of the Annatto tree (Bixa orellana L.). This is the first report in the literature that shows the relationship between the aqueous annatto extract and its influence on lipid profile in animals. Male Fisher rats were divided into three groups (n=12): C group, fed standard diet and water; H group, fed high-lipid diet and water and; HU group, with high-lipid diet and aqueous annatto extract for 60 days. The treatment with annatto extract in animals fed with the high-lipid diet lowered the LDL- and total cholesterol and raised the HDL-cholesterol, suggesting a hypocholesterolemic effect. Neither high-fat diet nor aqueous annatto extract had any significant effect on serum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Annatto; Bixa orellana; Cholesterol; Rat; Aqueous extract; Lipid profile.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600008
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Elimination of turbidity interference in serum iron colorimetric assay by enzymatic proteolysis BABT
Cardoso,Leonardo M.; Andrade,Milton H. G. de; Chianca Junior,Deoclécio A.; Silva,Marcelo Eustáquio; Pedrosa,Maria Lúcia.
We describe a modification in the commercial colorimetric method for the determination of serum iron by using Ferrozine® . The modification was proposed because during the conventional procedure, turbidity observed when the serum of animals submitted to surgery was used interfered with the assay. We added to the original method, a previous treatment of the serum with proteolytic enzymes. This modification was also tested using plasma samples, although this was not recommended when the original method was used. The results demonstrated that: a) the treatment with a mixture of trypsin and chymotrypsin was effective in order to eliminate turbidity; b) there was no difference between the standard curves obtained by the conventional and the modified method for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Iron assay; Enzymatic proteolysis; Interference.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300019
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Whey protein improves HDL/non-HDL ratio and body weight gain in rats subjected to the resistance exercise BABT
Teixeira,Kely Raspante; Silva,Marcelo Eustáquio; Neves,Leandro Xavier; Santos,Rinaldo Cardoso dos; Pedrosa,Maria Lúcia; Haraguchi,Fabiano Kenji.
The aim of this study was to evaluate the effects of resistance exercise, such as weight-lifting (WL) on the biochemical parameters of lipid metabolism and cardiovascular disease risk in the rats fed casein (control) or whey protein (WP) diets. Thirty-two male Fisher rats were randomly assigned to sedentary or exercise-trained groups and were fed control or WP diets. The WL program consisted of inducing the animals to perform the sets of jumps with weights attached to the chest. After seven weeks, arteriovenous blood samples were collected for analysis. The WL or WP ingestion were able to improve the lipid profile, reducing the TC and non-HDL cholesterol concentrations, but only WP treatment significantly increased the serum HDL concentrations, thereby...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dietary protein; Lipid profile; Cholesterol; Cardiovascular disease risk.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600019
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