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Registros recuperados: 9
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Agronomic performance, chromosomal stability and resistance to velvetbean caterpillar of transgenic soybean expressing cry1Ac gene PAB
Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Salvadori,José Roberto; Nicolau,Marcio; Kaltchuk-Santos,Eliane; Alves,Laura Bianchessi; Bodanese-Zanettini,Maria Helena.
The objective of this work was to analyze the agronomic performance and chromosomal stability of transgenic homozygous progenies of soybean [Glycine max (L.) Merrill.], and to confirm the resistance of these plants against Anticarsia gemmatalis. Eleven progenies expressing cry1Ac, hpt and gusA genes were evaluated for agronomic characteristics in relation to the nontransformed parent IAS 5 cultivar. Cytogenetical analysis was carried out on transgenic and nontransgenic plants. Two out of the 11 transgenic progenies were also evaluated, in vitro and in vivo, for resistance to A. gemmatalis. Two negative controls were used in resistance bioassays: a transgenic homozygous line, containing only the gusA reporter gene, and nontransgenic 'IAS 5' plants. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticarsia gemmatalis; Glycine max; Plant resistance to insects; Gene transfer.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700003
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Assessment of genetic diversity in Brazilian barley using SSR markers Genet. Mol. Biol.
Ferreira,Jéssica Rosset; Pereira,Jorge Fernando; Turchetto,Caroline; Minella,Euclydes; Consoli,Luciano; Delatorre,Carla Andréa.
Abstract Barley is a major cereal grown widely and used in several food products, beverage production and animal fodder. Genetic diversity is a key component in breeding programs. We have analyzed the genetic diversity of barley accessions using microsatellite markers. The accessions were composed of wild and domesticated barley representing genotypes from six countries and three breeding programs in Brazil. A total of 280 alleles were detected, 36 unique to Brazilian barley. The marker Bmag120 showed the greatest polymorphism information content (PIC), with the highest mean value found on chromosome three, and the lowest on chromosomes four and six. The wild accessions presented the highest diversity followed by the foreign genotypes. Genetic analysis was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Domesticated barley; Genetic diversity; Hordeum vulgare ssp. vulgare; Hordeum vulgare ssp. spontaneum; Microsatellite markers.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100086
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Development of a transformation system for Penicillium brevicompactum based on the Fusarium oxysporum nitrate reductase gene BJM
Varavallo,Maurílio Antônio; Queiroz,Marisa Vieira de; Pereira,Jorge Fernando; Ribeiro,Ronney Adriano; Soares,Marcos Antônio; Ribeiro,João Batista; Araújo,Elza Fernandes de.
Penicillium brevicompactum is a filamentous fungus that presents a potential for industrial use due its efficient pectinase production. A heterologous transformation system was developed for P. brevicompactum based on the complementation of a nitrate reductase mutant. Nitrate reductase mutants were obtained by resistance to chlorate in a rate of 23.24% when compared to other mutations that lead to the chlorate resistance. One mutant named 4457-18X was chosen for the transformation experiments with the pNH24 vector containing de Fusarium oxysporum nitrate reductase gene. A frequency of approximately 3 transformants/µg DNA was obtained using the circular vector pNH24. This frequency was multiplied about 10 fold using the linearized vector with the Xba I...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitrate reductase; Penicillium brevicompactum; Heterologous transformation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200015
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Gene expression analysis reveals important pathways for drought response in leaves and roots of a wheat cultivar adapted to rainfed cropping in the Cerrado biome Genet. Mol. Biol.
Poersch-Bortolon,Liane Balvedi; Pereira,Jorge Fernando; Nhani Junior,Antonio; Gonzáles,Hebert Hernán Soto; Torres,Gisele Abigail Montan; Consoli,Luciano; Arenhart,Rafael Augusto; Bodanese-Zanettini,Maria Helena; Margis-Pinheiro,Márcia.
Abstract Drought limits wheat production in the Brazilian Cerrado biome. In order to search for candidate genes associated to the response to water deficit, we analyzed the gene expression profiles, under severe drought stress, in roots and leaves of the cultivar MGS1 Aliança, a well-adapted cultivar to the Cerrado. A set of 4,422 candidate genes was found in roots and leaves. The number of down-regulated transcripts in roots was higher than the up-regulated transcripts, while the opposite occurred in leaves. The number of common transcripts between the two tissues was 1,249, while 2,124 were specific to roots and 1,049 specific to leaves. Quantitative RT-PCR analysis revealed a 0.78 correlation with the expression data. The candidate genes were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 454 sequencing; Candidate genes; RT-qPCR; Triticum aestivum; Water deficit.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400629
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Genetic diversity of Brazilian triticales evaluated with genomic wheat microsatellites PAB
Costa,Cibele Tesser da; Albuquerque,Ana Christina Sagebin; Nascimento Junior,Alfredo do; Marcelino,Francismar Correa; Pereira,Jorge Fernando.
The objective of this work was to determine the genetic variability available for triticale (X Triticosecale Wittmack) crop improvement in Brazil. Forty-two wheat genomic microsatellites were used to estimate the molecular diversity of 54 genotypes, which constitute the base of one of the major triticale breeding programs in the country. Average heterozygosity was 0.06 and average and effective number of alleles per locus were 2.13 and 1.61, respectively, with average allelic frequency of 0.34. The set of genomic wheat microsatellites used clustered the genotypes into seven groups, even when the germplasm was originated primarily from only two triticale breeding programs, a fact reflected on the average polymorphic information content value estimated for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: X Triticosecale; Polymorphism information content; Transferability; Heterozygosity; Number of alleles; Frequency of alleles.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007001100009
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Initial root length in wheat is highly correlated with acid soil tolerance in the field Scientia Agricola
Pereira,Jorge Fernando.
ABSTRACT: In acid soils, toxic aluminum ions inhibit plant root growth. In order to discriminate aluminum (Al) tolerance, trustful screening techniques are required. In this study, 20 wheat cultivars, showing different levels of Al tolerance, were evaluated in a short-term soil experiment to access their relative root length (RRL). Moreover, the alleles of two important genes (TaALMT1 and TaMATE1B) for Al tolerance in wheat were discriminated. Both of these genes encode membrane transporters responsible for the efflux of organic acids by the root apices that are thought to confer tolerance by chelating Al. Genotypes showing TaALMT1 alleles V and VI and an insertion at the TaMATE1B promoter were among the ones showing greater RRL. Mechanisms of Al...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Aluminum tolerance; Citrate and malate transporters; Short-term soil experiment.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000100079
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Is a non-synonymous SNP in the HvAACT1 coding region associated with acidic soil tolerance in barley? Genet. Mol. Biol.
Ferreira,Jéssica Rosset; Faria,Bruna Franciele; Comar Junior,Moacyr; Delatorre,Carla Andréa; Minella,Euclydes; Pereira,Jorge Fernando.
Abstract The barley HvAACT1 gene codes for a citrate transporter associated with tolerance to acidic soil. In this report, we describe a single nucleotide polymorphism (SNP) in the HvAACT1 coding region that was detected as T-1,198 (in genotypes with lower root growth on acidic soil) or G-1,198 (greater root growth) and resulted in a single amino acid change (L/V-172). Molecular dynamic analysis predicted that HvAACT1 proteins with L or V-172 were stable, although the substitution led to structural changes within the protein. To evaluate the effect of the SNP on tolerance to acidic soil, barley accessions were separated into haplotypes based on the presence of a 1 kb insertion in the HvAACT1 promoter and a 21 bp insertion/deletion. These markers and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aluminium tolerance; Citrate transporter; Haplotype; Hordeum vulgare; Single nucleotide polymorphism.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300480
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Pectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression BJM
Lima,Juliana Oliveira; Pereira,Jorge Fernando; Araújo,Elza Fernandes de; Queiroz,Marisa Vieira de.
Abstract Expression of pectinolytic genes is regulated by catabolic repression limiting the production of pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here, we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose (DG) that show resistance to catabolite repression and overproduce PL. Three spontaneous and nine UV-induced mutants were obtained. Some mutants produced sectors (segments morphologically different) that were also studied. The mutants were analyzed for pectinases production on pectinase-agar plates and five mutants and two sectors showing larger clearing zones than the wild type were selected for quantitative assay. Although PL production higher than the wild type has been...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-Deoxy-d-glucose; Ultraviolet mutagenesis; Pectinolytic complex; Spontaneous mutant.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300602
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Resistance to Anticarsia gemmatalis Hübner (Lepidoptera, Noctuidae) in transgenic soybean (Glycine max (L.) Merrill Fabales, Fabaceae) cultivar IAS5 expressing a modified Cry1Ac endotoxin Genet. Mol. Biol.
Homrich,Milena Schenkel; Passaglia,Luciane Maria Pereira; Pereira,Jorge Fernando; Bertagnolli,Paulo Fernando; Pasquali,Giancarlo; Zaidi,Mohsin Abbas; Altosaar,Illimar; Bodanese-Zanettini,Maria Helena.
Somatic embryos of the commercial soybean (Glycine max) cultivar IAS5 were co-transformed using particle bombardment with a synthetic form of the Bacillus thuringiensis delta-endotoxin crystal protein gene cry1Ac, the beta-glucuronidase reporter gene gusA and the hygromycin resistance gene hpt. Hygromycin-resistant tissues were proliferated individually to give rise to nine sets of clones corresponding to independent transformation events. The co-bombardment resulted in a co-transformation efficiency of 44%. Many histodifferentiated embryos and 30 well-developed plants were obtained. Twenty of these plants flowered and fourteen set seeds. The integration and expression of the cry1Ac, gusA and hpt transgenes into the genomes of a sample of transformed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticarsia gemmatalis; Cry1Ac gene; IAS5 cultivar; Insect resistance; Transgenic soybean.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300020
Registros recuperados: 9
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