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Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 7
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Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L. Biological Sciences
Santana, Gleuvânia Marques; Sousa, Josefa Janaína do Anjos; Peron, Ana Paula.
This study aimed to evaluate the toxicity of Ponceau 4R food dye on the cell cycle in root meristematic cells of Allium cepa L. at three concentrations: 0.25, 0.50 and 0.75 g L-1, at exposure times of 24 and 48 hours. For each concentration, we used a set of five onion bulbs that were first rooted in distilled water and then transferred to their respective concentrations. Radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were mounted with the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5,000 cells for each control and exposure time. The calculated mitotic indices were subjected to the Chi-square test (p < 0.05). From the results, we observed that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tecnolologia de alimentos citogenética azo dye; Cell division; Cellular aberrations; Cytotoxicity.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23119
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Cytotoxic and genotoxic potential of industrialized powdered milk for infants and young children Biological Sciences
Silva, Geysa Janne Sousa; Santos, Fabelina Karollyne Silva dos; Marques, Márcia Maria Mendes; Peron, Ana Paula.
This study aimed to evaluate the cytotoxicity and genotoxicity and determine the LC50 concentration of powdered infant formulas widely marketed in South American countries. To this, milk samples, called as A, B, C and D, were analyzed in root meristem cells of Allium cepa, at concentrations of 0.075; 0.15 and 0.30 g mL-1, for 24 and 48 hours; and through cell viability in culture of normal line cells, via MTT test, for 24 hours, in the concentrations 0.018; 0.0375; 0.075 and 0.15 g mL-1. In A. cepa, all dairy products in the three concentrations caused significant inhibition of cell division in the meristems within the first 24 hours of exposure. In the in vitro evaluation, all milk formulas at 0.15 g mL-1, as well as milk A at a concentration of 0.037 g...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Infant formula; Cell division; Meristematic tissue; Cell viability; LC50.
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46856
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Karyotypic characterization of Capsicum sp. accessions Agronomy
Souza, Willame Rodrigues do Nascimento; Almeida, Angela Celis de; Carvalho, Reginaldo de; Ferreira, Regina Lúcia; Peron, Ana Paula.
This study aimed to determine the chromosome number and the karyotype of Capsicum annuum, Capsicum chinense, Capsicum frutencens and Capsicum baccatum accessions in the active Capsicum sp. genebank at the Federal University of Piauí (BGC-UFPI). These species have great economic importance throughout the world, and their cytogenetic characterization can inform taxonomy and lead to improvement in the genus. Karyotypes were obtained from the rootlet meristems of the studied accessions using the squash method and Giemsa staining. The chromosome number 2n=2x=24 was verified for each of the four species. Chromosomal polymorphisms were observed for the C. frutencens accession BGC 37, which presented 12 pairs of metacentric chromosomes instead of 11 pairs of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CITOGENÉTICA pepper; Cytogenetics; Genebank; Morphometry CITOGENÉTICA DE CULTIVARES DE INTERESSE ECONÔMICO.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19485
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Processed fruit juice ready to drink: screening acute toxicity at the cellular level Biological Sciences
Silva, Erick Leal da; Sales, Ila Monize Sousa; Santos, Fabelina Karollyne Silva dos; Peron, Ana Paula.
The present study evaluated the acute toxicity at the cellular level of processed juice ready for consumption Orange and Grape flavors, produced by five companies with significant influence on the food market of South American countries, especially in Brazil. This evaluation was performed in root meristem cells of Allium cepa L., at the exposure times of 24 and 48 hours, directly with marketed liquid preparations. Based on the results, it was found that fruit juices, of all companies considered, promoted significant antiproliferative effect to root meristems at the exposure time of 24 hours and resulted in at both exposure times, statistically significant number of mitotic spindle changes and chromosomal breaks. Therefore, under the study conditions, all...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Industrialized juice; Cytotoxicity; Genotoxicity; Mutagenicity; Meristem tissue.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33699
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Toxicity at the cellular level of artificial synthetic flavorings Biological Sciences
Sales, Ila Monize Sousa; Oliveira, Jussara Damascena de; Santos, Fabelina Karollyne Silva dos; Feitoza, Lidiane de Lima; Sousa, João Marcelo de Castro e; Peron, Ana Paula.
The goal of the present study was to evaluate the cytotoxicity and genotoxicity of artificial synthetic flavoring agents Cookie and Tutti-frutti. To this end, root meristem cells of Allium cepa L. were exposed to these substances in exposure times of 24 and 48 hours using individual doses of 0.3; 0.6 and 0.9 mL and doses combined as follows: 0.3 mL + 0.3 mL; 0.6 mL and 0.9 mL + 0.6 mL + 0.9 mL. After applying the treatments, root meristems were fixed, hydrolyzed, stained and analyzed a total of 5,000 cells using an optical microscope to evaluate each dose and combined treatment. All three doses of Cookie flavoring and combined treatments significantly inhibited cell division of the tissue studied. Doses of Tutti-Frutti caused no change in cell division...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flavorings; Cell division; Cell aberrations; Cytotoxicity; Genotoxicity flavorings; Cell division; Cell aberrations; Cytotoxicity; Genotoxicity.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30751
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Toxicity in food flavorings at the cellular level associated with each other at different doses Biological Sciences
Silva, Sara Iolanda Oliveira da; Santana, Gleuvânia Marques; Sales, Ila Monize Sousa; Sousa, João Marcelo de Castro e; Peron, Ana Paula.
This study aimed to examine the cytotoxicity and genotoxicity of synthetic flavorings, nature identical, Chocolate, Strawberry and Condensed Milk. This evaluation was performed in root meristem cells of Allium cepa L., in exposure times of 24 and 48 hours and using doses of 0.2; 0.4 and 0.6 mL, in combination, in which one of the three doses of a flavoring was combined with a different dose of one of the two other flavor additives studied. Roots were fixed in Carnoy’s solution, hydrolyzed in hydrochloric acid, stained with acetic orcein and then analyzed, under light microscopy, 5,000 cells for each treatment. For data analysis, it was used Chi-square test at 5%. All the treatments with combinations between the flavorings Chocolate/Strawberry and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aroma and flavor additives; Cytotoxicity; Genotoxicity A. cepa Citogenética.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30475
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Toxicity of the goji berry fruit associated with artificial excipients and dried without additives Biological Sciences
Moura, Débora Dayane Araújo de; Veloso, Cleidiane Josefa dos Santos; Oliveira, Valtânia Ana de; Silva, Maria Eduarda Sousa e; Peron, Ana Paula.
 The aim of this study was to evaluate the cytotoxic and genotoxic potential of goji berry fruit-based pharmaceutical powders obtained from three pharmaceutical laboratories. The product A was tested at concentrations of 0.012; 0.025 and 0.05 g mL-1, and B and C at concentrations 0.02; 0.04 and 0.08 g mL-1. It was also evaluated the tea of the dried goji berry fruit (non-additives) in the concentrations 0.035; 0.07 and 0.14 g mL-1 for comparison to the results obtained with powdered goji berry. Tea concentrations in the two exposure times did not cause inhibition of cell division nor cellular alterations to meristem tissues. For the industrialized goji products, all concentrations analyzed caused significant antiproliferative effect to the tissues...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycium barbarum L.; Natural pharmaceutical product; Cytotoxicity; Genotoxicity; Meristematic tissue..
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37844
Registros recuperados: 7
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