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| Registros recuperados: 59 | |
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| Goudenege, David; Travers, Marie-agnes; Lemire, Astrid; Petton, Bruno; Haffner, Philippe; Labreuche, Yannick; Tourbiez, Delphine; Mangenot, Sophie; Calteau, Alexandra; Mazel, Didier; Nicolas, Jean-louis; Jacq, Annick; Le Roux, Frederique. |
| Oyster diseases caused by pathogenic vibrios pose a major challenge to the sustainability of oyster farming. In France since 2012 a disease affecting specifically adult oysters has been associated with the presence of Vibrio aestuarianus. Here, by combining genome comparison, phylogenetic analyses and high throughput infections of strains isolated before or during the recent outbreaks, we show that virulent strains cluster into two V. aestuarianus lineages independently of the sampling dates. The bacterial lethal dose was not different between strains isolated before or after 2012. Hence the emergence of a new highly virulent clonal strain is unlikely. Each lineage comprises nearly identical strains, the majority of them being virulent, suggesting that... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00226/33685/32530.pdf |
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| Lafont, Maxime; Vergnes, Agnes; Vidal-dupiol, Jeremie; De Lorgeril, Julien; Gueguen, Yannick; Haffner, Philippe; Petton, Bruno; Chaparro, Cristian; Barrachina, Celia; Destoumieux-garzon, Delphine; Mitta, Guillaume; Gourbal, Benjamin; Montagnani, Caroline. |
| Over the last decade, innate immune priming has been evidenced in many invertebrate phyla. If mechanistic models have been proposed, molecular studies aiming to substantiate these models have remained scarce. We reveal here the transcriptional signature associated with immune priming in the oyster Crassostrea gigas. Oysters were fully protected against Ostreid herpesvirus 1 (OsHV-1), a major oyster pathogen, after priming with poly(I·C), which mimics viral double-stranded RNA. Global analysis through RNA sequencing of oyster and viral genes after immune priming and viral infection revealed that poly(I·C) induces a strong antiviral response that impairs OsHV-1 replication. Protection is based on a sustained upregulation of immune genes, notably genes... |
| Tipo: Text |
Palavras-chave: Innate immunity; Priming; OsHV-1; Antiviral response; Immune memory; Oyster; POMS; Poly(I·C); Interferon; Transcriptomic. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00614/72580/71541.pdf |
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| Bruto, Maxime; Labreuche, Yannick; James, Adele; Piel, Damien; Chenivesse, Sabine; Petton, Bruno; Polz, Martin F.; Le Roux, Frederique. |
| Diseases of marine animals caused by bacteria of the genus Vibrio are on the rise worldwide. Understanding the eco-evolutionary dynamics of these infectious agents is important for predicting and managing these diseases. Yet, compared to Vibrio infecting humans, knowledge of their role as animal pathogens is scarce. Here we ask how widespread is virulence among ecologically differentiated Vibrio populations, and what is the nature and frequency of virulence genes within these populations? We use a combination of population genomics and molecular genetics to assay hundreds of Vibrio strains for their virulence in the oyster Crassostrea gigas, a unique animal model that allows high-throughput infection assays. We show that within the diverse Splendidus... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00452/56370/57967.pdf |
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| Cochennec-laureau, Nathalie; Baud, Jean-pierre; Bedier, Edouard; Boudry, Pierre; Huvet, Arnaud; Nicolas, Jean-louis; Pepin, Jean-francois; Petton, Bruno. |
| Ce bilan comprend deux parties : la première est dédiée à une synthèse des travaux présentés lors des journées « Surmortalités des huîtres creuses, Crassostrea gigas » les 8 et 9 décembre 2009 et, la seconde partie, à une proposition d'un projet de recherche spécifique 2010-2012 en complément des propositions de « sortie de crise ». Contexte Les mortalités estivales de l'huître creuse, Crassostrea gigas, sont décrites depuis une vingtaine d'années en France mais également au Japon, en Corée, aux USA et en Australie. Ces phénomènes ou « syndrome des mortalités estivales » ont fait l'objet d'une étude pluridisciplinaire en France entre 2001 et 2006 (Défi MOREST). Toutefois, en 2008 et 2009, l'ostréiculture française a du faire face à deux épisodes de... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/2010/acte-7393.pdf |
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| Suquet, Marc; Omnes, Marie-helene; Normant, Yvon; Petton, Bruno; Severe, Armelle; Fauvel, Christian; Barone, Herve; Quemener, Loic; Buchet, Vincent; Pasco, Laurent; Menard, E; Gaignon, Jean-louis. |
| Because of its rapid growth, its good reputation and the promising aquaculture experiences conducted in Norway and Scotland, cod is considered as a good candidate for cold water aquaculture. The aim of this paper is to present the work carried out in France since 1999: the selection of cod as a promising candidate for aquaculture and the assessment of its rearing performances in this environment. |
| Tipo: Text |
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| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00170/28142/26362.pdf |
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| Pernet, Fabrice; Lugue, Klervi; Petton, Bruno. |
| Competition between organisms interfere in host and pathogen dynamics in ways that are difficult to predict. By one side, competitors can reduce the food supply and cause nutritional stress. Such stress could further modulate the susceptibility to infection by altering immune response or metabolic rate of the host. Alternatively, competitors may trap pathogens before they reach the focal host, and therefore reduce, enhance, or have no effect on infection according to the competitor's susceptibility to the infection. To better understand how competition influences host and pathogen interactions, we experimentally assessed the relative importance of competition for pathogens and resources on the severity of a viral disease infecting the Pacific oyster... |
| Tipo: Text |
Palavras-chave: Competition; Crassostrea gigas; Disease ecology; Filtration; Ostreid herpesvirus 1; Pathogen. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00692/80375/83493.pdf |
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| Gangnery, Aline; Normand, Julien; Duval, Cyrielle; Cugier, Philippe; Grangeré, Karine; Petton, Bruno; Petton, Sebastien; Orvain, Francis; Pernet, Fabrice. |
| Oyster diseases have major consequences on fisheries and aquaculture. In France, young Pacific oysters Crassostrea gigas are severely hit by the ostreid herpesvirus, whereas adults suffer mortalities presumably caused by pathogenic bacteria. Here we investigated the origin and spread of mortalities that affect both young and adult oysters, and we identified and compared their risk factors. Mortality was monitored in 2 age classes of oysters deployed in early spring at 39 sites spread over a 37 km2 surface area inside and outside of shellfish farms. Environmental data obtained from numerical modelling were used to investigate risk factors. Mortality of young oysters associated with ostreid herpesvirus occurred in the oyster farming area. Hydrodynamic... |
| Tipo: Text |
Palavras-chave: Aquaculture; Bivalve; Disease; Epidemiology and health; Ecological modelling; Hydrodynamic connectivity. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00588/69983/67896.pdf |
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| Delmotte, Jean; Chaparro, Cristian; Galinier, Richard; De Lorgeril, Julien; Petton, Bruno; Stenger, Pierre-louis; Vidal-dupiol, Jeremie; Destoumieux-garzon, Delphine; Gueguen, Yannick; Montagnani, Caroline; Escoubas, Jean Michel; Mitta, Guillaume. |
| Juvenile Pacific oysters (Crassostrea gigas) are subjected to recurrent episodes of mass mortalities that constitute a threat for the oyster industry. This mortality syndrome named “Pacific Oyster Mortality Syndrome” (POMS) is a polymicrobial disease whose pathogenesis is initiated by a primary infection by a variant of an Ostreid herpes virus named OsHV-1 μVar. The characterization of the OsHV-1 genome during different disease outbreaks occurring in different geographic areas has revealed the existence of a genomic diversity for OsHV-1 μVar. However, the biological significance of this diversity is still poorly understood. To go further in understanding the consequences of OsHV-1 diversity on POMS, we challenged five biparental families of oysters to two... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Herpesvirus diversity; Genotype-genotype interactions; Oyster genetic background; Viral populations. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00643/75533/76414.pdf |
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| Petton, Bruno; Bruto, Maxime; James, Adele; Labreuche, Yannick; Alunno-bruscia, Marianne; Le Roux, Frederique. |
| Successive disease outbreaks in oyster (Crassostrea gigas) beds in France have resulted in dramatic losses in production, and subsequent decline in the oyster-farming industry. Deaths of juvenile oysters have been associated with the presence of a herpes virus (OsHV-1 µvar) and bacterial populations of the genus Vibrio. Although the pathogenicity of OsHV-1 µvar, as well as several strains of Vibrio has been demonstrated by experimental infections, our understanding of the complexity of infections occurring in the natural environment remains limited. In the present study, we use specific-pathogen-free (SPF) oysters infected in an estuarine environment to study the diversity and dynamics of cultured microbial populations during disease expression. We observe... |
| Tipo: Text |
Palavras-chave: Pacific oysters; Summer mortality; Herpes virus; Vibrio pathogenicity; Experimental infection. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00271/38220/36377.pdf |
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| Pernet, Fabrice; Tamayo, David; Fuhrmann, Marine; Petton, Bruno. |
| Food provisioning influences disease risk and outcome in animal populations in two ways. On the one hand, unrestricted food supply improves the physiological condition of the host and lowers its susceptibility to infectious disease, reflecting a trade-off between immunity and other fitness-related functions. On the other hand, food scarcity limits the resources available to the pathogen and slows the growth and metabolism of the host on which the pathogen depends to proliferate. Here, we investigated how food availability, growth rate and energetic reserves drive the outcome of a viral disease affecting an ecologically relevant model host, the Pacific oyster, Crassostrea gigas. We selected fast- and slow-growing animals, and we exposed them to high and low... |
| Tipo: Text |
Palavras-chave: Energetics; Health; Herpesvirus; Nutrition; Physiological trade-off. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00513/62431/66716.pdf |
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| Pernet, Fabrice; Fuhrmann, Marine; Petton, Bruno; Mazurie, Joseph; Bouget, Jean-francois; Fleury, Elodie; Daigle, Gaetan; Gernez, Pierre. |
| Marine diseases have major impacts on ecosystems and economic consequences for aquaculture and fisheries. Understanding origin, spread and risk factors of disease is crucial for management, but data in the ocean are limited compared to the terrestrial environment. Here we investigated how the marine environment drives the spread of viral disease outbreak affecting The Pacific oyster worldwide by using a spatial epidemiology framework. We collected environmental and oyster health data at 46 sites spread over an area of 300 km2 along an inshore-offshore gradient during an epizootic event and conducted risk analysis. We found that disease broke out in the intertidal farming area and spread seaward. Mortalities and virus detection were observed in oysters... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00450/56130/57670.pdf |
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| Asmani, Katia; Petton, Bruno; Le Grand, Jacqueline; Mounier, Jerome; Robert, Rene; Nicolas, Jean-louis. |
| The first aim of this study was to determine the stocking density limits for Pacific oyster Crassostrea gigas larvae reared in flow-through system (FTS) and recirculating aquaculture systems (RAS). The second aim was to examine biofilm formation on the larval tank wall and its interaction with larvae growth. Three larvae concentrations were tested: 50, 150, and 300 mL−1. Chemical parameters and larvae performance were measured. The biofilm was observed by scanning electron microscopy, and its bacterial composition was investigated by pyrosequencing analysis of part of the 16S rRNA gene. The highest growth (13 µm day–1), survival (87%) and metamorphosis (50%) rates were observed in FTS at 50 larvae mL–1, while lower and similar performances occurred at... |
| Tipo: Text |
Palavras-chave: Pacific oyster; Larval culture; High stocking density; Biofilm; Filamentous bacteria. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00395/50611/51324.pdf |
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| De Lorgeril, Julien; Petton, Bruno; Lucasson, Aude; Perez, Valérie; Stenger, Pierre-louis; Degremont, Lionel; Montagnani, Caroline; Escoubas, Jean Michel; Haffner, Philippe; Allienne, Jean-françois; Leroy, Marc; Lagarde, Franck; Vidal Dupiol, Jeremie; Gueguen, Yannick; Mitta, Guillaume. |
| Background: As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). Results: We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein... |
| Tipo: Text |
Palavras-chave: Pacific oyster; Oyster disease; Resistance; OsHV-1; Antiviral molecular pathways; Invertebrate immunity. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00602/71369/69811.pdf |
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| Asmani, Katia; Petton, Bruno; Le Grand, Jacqueline; Mounier, Jerome; Robert, Rene; Nicolas, Jean-louis. |
| This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS with 25% fresh seawater addition per hour and another with no addition (0%) were compared with a flow-through system (FT). Larval survival was equivalent in RS and FT, but growth rate was 17% slower in RS than in FT. The physical chemical parameters remained stable, except for pH that decreased to 7.75 and salinity that increased to 37.5‰ in the RS 0%. In both systems, the cultivable bacteria were present in similar numbers in seawater (around 105 ml− 1) and in larvae (103 larva− 1) on day 15. Bacterial... |
| Tipo: Text |
Palavras-chave: RAS; Bivalve larvae; Microbiota; Pyrosequencing; Hatchery. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00346/45691/45311.pdf |
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| Petton, Bruno; Boudry, Pierre; Alunno-bruscia, Marianne; Pernet, Fabrice. |
| Mortalities of oyster Crassostrea gigas seed associated with ostreid herpesvirus OsHV-1 μVar have been observed in many oyster-producing countries since 2008. The present study, comprised of 4 complementary experiments, aimed to identify factors associated with disease-induced oyster mortality in order to propose mitigation strategies. Our first experiment compared survival of oysters from natural spatfall with others sampled from nurseries, after thermal elevation in the laboratory from <14 to 21°C. A total of 60% of the tested wild seed batches (n = 51) were infected by OsHV-1, exhibited mortality and were able to transmit the disease to cohabited naïve oysters. Comparatively, only 1 out of the 32 tested batches sampled from nurseries presented... |
| Tipo: Text |
Palavras-chave: Epidemiology; Risk analyses; Shellfish farming; Virus OsHV-1 mu Var. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00257/36777/35390.pdf |
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| Pernet, Fabrice; Gachelin, Sonia; Stanisière, Jean-yves; Petton, Bruno; Fleury, Elodie; Mazurié, Joseph. |
| The intertidal zone is characterized by a sharp vertical gradient of environmental stress, which structures species distribution and their interactions. Few studies, however, have examined the influence of tidal height on host–pathogen interactions. Here, we investigated how the tidal height influence outbreak of the Ostreid herpesvirus type 1 (OsHV-1) affecting the Pacific oyster. A volunteer network composed of 20 oyster growers monitored the survival of 28 batches of oysters during an epizootic event in Southern Brittany, France. Oysters were spat from wild collection or hatchery production. The sampling sites were spread over a 150-km2 area with a tidal height ranging from 0.98 to 2.90 m. Concomitantly, we followed survival of oyster spats in relation... |
| Tipo: Text |
Palavras-chave: Aquaculture; Citizen science; Marine epidemiology and health; OsHV-1; Risk analysis. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00491/60271/63981.pdf |
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| Registros recuperados: 59 | |
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