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Registros recuperados: 16
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Expression of a poplar cDNA encoding a ferulate-5-hydroxylase/coniferaldehyde 5-hydroxylase increases S lignin deposition in Arabidopsis thaliana Inra
Sibout, R.; Baucher, M.; Gatineau, M.; Van Doorsselaere, J.; Mila, I.; Pollet, B.; Maba, B.; Pilate, G.; Lapierre, C.; Boerjan, W.; Jouanin, L..
Ferulate-5-hydroxylase or coniferaldehyde 5-hydroxylase (F5H or Cald5H; CYP84A1) is the enzyme responsible for the lasthydroxylation of the syringyl-type lignin precursors. A cDNA clone highly homologous to the Arabidopsis thaliana F5H/Cald5H cDNAwas identified during the random sequencing of a poplar (Populus trichocarpa) differentiating xylem cDNA library. Present as a multigenicfamily in poplar, this gene (PopF5H) was found to be highly expressed in lignified tissues supporting its role in lignin biosynthesis. Whenplaced under the control of the CaMV 35S promoter, the poplar F5H was able to complement the A. thaliana fah1-2 mutation.Overexpression in wild-type A. thaliana substantially increased the level of syringyl units in lignins. These transgenic...
Tipo: Journal Article Palavras-chave: BIOSYNTHESE DE LA LIGNINE; EXPRESSION DES GENES; ADNC FERULATE 5-HYDROXYLASE; POPULUS TRICHOCARPA; ARABIDOPSIS THALIANA.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007a66658c5&uri=/notices/prodinra1/2008/12/
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Genetically modified lignin below ground Inra
Hopkins, D.W.; Webster, E.A.; Boerjan, W.; Pilate, G.; Halpin, C..
Tipo: Journal Article Palavras-chave: DEGRADATION DU BOIS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007b7c341a1&uri=/notices/prodinra1/2007/07/
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Genome-wide analysis of LIM gene family in Populus trichocarpa, Arabidopsis thaliana and Oryza sativa Inra
Arnaud, D.; Dejardin, A.; Leplé, J.C.; Lesage Descauses, M.C.; Pilate, G..
In Eukaryotes, LIM proteins act as developmental regulators in basic cellular processes such as regulating the transcription or organizing the cytoskeleton. The LIM domain protein family in plants has mainly been studied in sunflower and tobacco plants, where several of its members exhibit a specific pattern of expression in pollen. In this paper, wefinely characterized in poplar six transcripts encoding these proteins. In Populus trichocarpa genome, the 12 LIMgene models identified all appear to be duplicated genes. In addition, we describe several new LIM domain proteinsdeduced from Arabidopsis and rice genomes, raising the number of LIM gene models to six for both species. PlantLIM genes have a core structure of four introns with highly conserved coding...
Tipo: Journal Article Palavras-chave: BOIS DE TENSION; SEQUENCE DE GENES; ETIQUETTE DE SEQUENCE TRANSCRITE; ANALYSE DU GENOME TENSION WOOD; GENE SEQUENCE; EXPRESSED SEQUENCE TAG; GENOME ANALYSIS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200976260545&uri=/notices/prodinra1/2009/05/
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High tolerance against Chrysomela tremulae of transgenic poplar plants expressing a synthetic cry3Aa gene from Bacillus thuringiensis ssp tenebrionis Inra
Génissel, A.; Leplé, J.C.; Millet, N.; Augustin, S.; Jouanin, L.; Pilate, G..
Hybrid poplars (Populus tremula ×Populus tremuloides) have been genetically engineered viaAgrobacterium tumefaciens, to express a syntheticcry3Aa gene derived from the native Bacillusthuringiensis subsp. tenebrionis cry3Aa gene.The presence and the expression of the transgene have been verified in fourtransgenic poplar lines, using Southern, northern and western analyses. Thetransgenic poplar's toxicity towards the phytophagous beetleChrysomela tremulae (Coleoptera, Chrysomelidae) has beenassessed on six month-old greenhouse-grown selected plants in laboratoryconditions. Laboratory experiments consisted of feeding tests of fresh detachedleaves on C. tremulae at all developmental stages. Ourresults indicate that the transgenic poplar leaves, expressing a...
Tipo: Journal Article Palavras-chave: PEUPLIER TRANSGENIQUE; RESISTANCE AUX INSECTES NUISIBLES; EXPRESSION DES GENES; TOXICITE DES FEUILLES BACILLUS THURINGIENSIS; CHRYSOMELA TREMULAE.
Ano: 2003 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007fc73fa80&uri=/notices/prodinra1/2007/06/
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Histological aspects of a hypersensitive response in Poplar to Melampsora larici-populina Inra
Laurans, F.; Pilate, G..
The course of the infection and development of the biotrophic fungus Melampsora larici-populina on leaf tissue from the hybrid poplar Populus deltoides × P. nigra ‘Ogy’ was monitored at the histological level. Leaf disks were inoculated with one of two rust physiological races (E1 and E2), resulting in interactions that were either incompatible (race E1) or compatible (race E2). In the compatible interaction, the fungus rapidly colonized the leaf without inducing any apparent host response. Symptoms appeared on the leaf several days after inoculation just prior to spore dissemination. The incompatible interaction was characterized by the early collapse and disorganization of cytoplasm of infected cells 17 h after inoculation and within 2 h after the...
Tipo: Journal Article Palavras-chave: PEUPLIER HYBRIDE; RESISTANCE A LA ROUILLE; REPONSE HYPERSENSIBLE; RACE PHYSIOLOGIQUE; ANALYSE HISTOLOGIQUE HYBRID POPLAR; RUST RESISTANCE; HYPERSENSITIVE RESPONSE; PHYSIOLOGICAL RACE; HISTOLOGICAL ANALYSIS.
Ano: 1999 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20109a00378b&uri=/notices/prodinra1/2011/02/
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Identification of the structure and origin of thioacidolysis marker compounds for cinnamyl alcohol dehydrogenase deficiency in angiosperms Inra
Kim, H.; Ralph, J.; Lu, F.; Pilate, G.; Leplé, J.C.; Pollet, B.; Lapierre, C..
Molecular marker compounds, derived from lignin by the thioacidolysis degradative method, for cinnamyl alcohol dehydrogenase (CAD) deficiency in angiosperms have been structurally identified as indene derivatives. They are shown to derive from hydroxycinnamyl aldehydes that have undergone 8-O-4-cross-coupling during lignification. As such, they are valuable markers for ascertaining plant responses to various levels of CAD down-regulation. Their derivation illustrates that hydroxycinnamyl aldehydes incorporate into angiosperm lignins by endwise coupling reactions in much the same way as normal monolignols do, suggesting that the hydroxycinnamyl aldehydes should be considered authentic lignin precursors.
Tipo: Journal Article Palavras-chave: BIOSYNTHESE DE LA LIGNINE.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007f4cdd854&uri=/notices/prodinra1/2010/11/
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Les biotechnologies chez les arbres forestiers Inra
Pilate, G.; Pâques, M.; Leplé, J.C.; Plomion, C..
Le développement actuel des biotechnologies ouvre des potentialités importantes en matière d'amélioration génétique des arbres forestiers. Notamment, celles-ci devraient permettre de diffuser rapidement les nouvelles variétés aux performances accrues nécessaires au maintien de la compétitivité de la filière-bois. Cette revue fait le point sur les possibilités et les limitations de l'utilisation des biotechnologies chez les arbres forestiers:multiplication végétative in vitro par embryogénèse somatique et par microbouturage, transformation génétique et sélection assistée par marqueurs.
Tipo: Journal Article Palavras-chave: BIOTECHNOLOGIE; AMELIORATION DES ARBRES FORESTIERS; EMBRYOGENESE SOMATIQUE; MICROBOUTURAGE.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20075239aed&uri=/notices/prodinra1/2008/11/
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Modified lignification in the cell walls of cad depressed poplars Inra
Yoshinaga, A.; Wada, M.; Fujita, M.; Chabbert, B.; Pilate, G..
Ultraviolet microscopic spectrophotometry was used to investigate lignificationin the secondary cell walls of wood fibers and vessel elementsfrom either wild type or transgenic poplars with depressed activity ofcinnamyl alcohol dehydrogenase (CAD). A distinct shoulder at 330 nmwas characteristic of the transgenic poplar. Measurements performedafter 1) a mild alkali treatment or 2) a reduction with sodium borohydrideindicated that this shoulder merely resulted from the occurrence of conjugatedcarbonyl groups in the lignin polymer rather than alkali-solublecell wall bound phenolic aldehydes. UV absorbance ratios (A330 /A280)measured in the center of secondary walls in the differentiating xylemclearly showed that the structural changes observed in the lignin...
Tipo: Journal Article Palavras-chave: PEUPLIER TRANSGENIQUE; ALCOOL CINNAMYLIQUE DESHYDROGENASE; SPECTROPHOTOMETRIE TRANSGENIC POPLAR; CELL WALL; WOOD FIBER; CINNAMYL ALCOHOL DESHYDROGENASE; CAD.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20097e76961a&uri=/notices/prodinra1/2009/09/
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NMR Evidence for benzodioxane structures resulting from incorporation of 5-hydroxyconiferyl alcohol into lignins of 0-methyltransferase-deficient poplars Inra
Ralph, J.; Lapierre, C.; Lu, F.; Marita, J. M.; Pilate, G.; Van Doorsselaere, J.; Boerjan, W.; Jouanin, L..
Benzodioxane structures are produced in lignins of transgenic poplar plants deficient in COMT, an O-methyltransferase required to produce lignin syringyl units. They result from incorporation of 5-hydroxyconiferyl alcohol into the monomer supply and confirm that phenols other than the three traditional monolignols can be integrated into plant lignins.
Tipo: Journal Article Palavras-chave: LIGNINE; LIGNANE; PLANTE TRANSGENIQUE; RMN; RESONANCE MAGNETIQUE NUCLEAIRE LIGNIN; LIGNAN; TRANSGENIC PLANT; NMR.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007fbcdf348&uri=/notices/prodinra1/2008/11/
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Physiologie de la formation des parois de fibres de bois Inra
Laurans, F.; Dejardin, A.; Leple, J.C.; Pilate, G..
Les feuillus possèdent un bois hétéroxylé, de structure complexe, constitué principalement de fibres, de vaisseaux et de rayons. Les fibres jouent un rôle primordial dans le soutien mécanique de l'arbre et dans sa capacité à développer des axes de grande taille. Les fibres résultent du fonctionnement d'une assise génératrice appelée cambium et située sous l'écorce de l'arbre entre le bois et le liber. La paroi des fibres est composée de plusieurs couches, formées d'un réseau de microfibrilles de cellulose cimenté dans une matrice d'hémicelluloses et de lignines. Les couches diffèrent par leur épaisseur, leur degré de lignification et l'orientation des microfibrilles de cellulose. La paroi des fibres peut être comparée à un matériau composite dont les...
Tipo: Journal Article Palavras-chave: HEMICELLULOSE; FORMATION DU BOIS.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20073d0357d7&uri=/notices/prodinra1/2007/04/
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Poplar genes encoding fasciclin-like arabinogalactan proteins are highly expressed in tension wood Inra
Lafarguette, F.; Leple, J.C.; Dejardin, A.; Laurans, F.; Costa, G.; Lesage Descauses, M.C.; Pilate, G..
• Fifteen poplar cDNA encoding fasciclin-like arabinogalactan proteins (PopFLAs) were finely characterized, whereas the presence of arabinogalactan proteins (AGPs) was globally assessed during wood formation.• PopFLAs transcript accumulation was analysed through EST distribution in cDNA libraries, semi-quantitative RT-PCR, microarray experiment and Northern blot analysis. Similarly, AGPs contents were globally quantified by rocket electrophoresis. AGPs accumulation was further examined by Western blotting and immunocytolocalization.• Ten PopFLAs were specifically expressed in tension wood (TW) and not expressed in the cambial zone. Rocket electrophoresis revealed important AGPs accumulation in TW xylem. An anti-AGPs specific antibody recognized two...
Tipo: Journal Article Palavras-chave: ARABINOGALACTANE; PROTEINE AGP; EXPRESSION DES GENES; BOIS DE TENSION.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200780b481d6&uri=/notices/prodinra1/2007/05/
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Quantitative 31P NMR spectroscopy of lignins from transgenic poplars Inra
Akim, L. G.; Argyropoulos, D. S.; Jouanin, L.; Leplé, J.C.; Pilate, G.; Pollet, B.; Lapierre, C..
Milled wood lignins isolated from genetically modified poplar trees were studied by quantitative 31P NMR spectroscopy, in combination with thioacidolysis and mild alkaline hydrolysis. The genetictransformation of the trees included down regulation of CAD and COMT enzyme activities.These analyses confirmed that moderate CAD down-regulation does not substantially alter ligninstructure. In contrast, severe CAD deficiency alters the lignin structure more profoundly by decreasingthe syringyl/guaiacyl ratio and increasing the degree of condensation of the lignin. Themost severe alterations were observed in the lignins from the COMT-transformed lines.
Tipo: Journal Article Palavras-chave: PEUPLIER TRANSGENIQUE; RMN; RESONANCE MAGNETIQUE NUCLEAIRE; THIOACIDOLYSE; HYDROLYSE ALCALINE TRANSGENIC POPLAR; NMR; NUCLEAR MAGNETIC RESONANCE; THIOACIDOLYSIS; ALKALINE HYDROLYSIS.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20071e4afa41&uri=/notices/prodinra1/2008/12/
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Stability of transgene expression in poplar: A model forest tree species Inra
Hawkins, S.; Leplé, J.C.; Cornu, D.; Jouanin, L.; Pilate, G..
Notre étude a pour but d'évaluer la stabilité de l'expression d'un transgène chez un peuplier hybride (Populus tremula x P. alba) transformé avec le gène reporter uidA sous le contrôle soit du promoteur 35S, soit du promoteur du gène cad2 de l'eucalyptus (EuCAD) à spécificité vasculaire. L'expression du transgène a été suivie quantitativement par fluorimétrie et qualitativement par histochimie à partir de matériel collecté in vitro, en serre ou en champ sur quelques centaines d'arbres, issus de 44 lignées transgéniques. Tandis qu'il existe d'importantes variations dans les niveaux d'expression du transgène selon les lignées, les arbres d'une même lignée, l'organe analysé ou la date de prélèvement, l'expression du transgène s'est révélée être stable sur une...
Tipo: Journal Article Palavras-chave: PEUPLIER TRANSGENIQUE; ESPECE MODELE; STABILITE; EXPRESSION DES GENES; TRANSGENE.
Ano: 2003 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007245d82c2&uri=/notices/prodinra1/2009/12/
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The CP4 transgene provides high levels of tolerance to Roundup® herbicide in field-grown hybrid poplars Inra
Meilan, R.; Han, K.H.; Ma, C.; DiFazio, S. P.; Eaton, J. A.; Hoien, E. A.; Stanton, B. J; Crockett, R. P.; Taylor, M. L.; James, R. R.; Skinner, J. S.; Jouanin, L.; Pilate, G.; Strauss, S. H..
Les auteurs ont étudié l’effet combiné de deux gènes chez des peupliers hybrides (genre Populus), CP4 et GOX, qui confèrent la tolérance au glyphosate (l’ingrédient actif de l’herbicide Roundup©). À l’aide de la transformation par Agrobacterium, 80 lignées transgéniques indépendantes (i.e., les produits du transfert génétique asexué) ont été produites à partir d’une variété de clones de peupliers hybrides (40 lignées de Populus trichocarpa Torr. & Gray × Populus deltoides, 35 lignées de Populus tremula L. × Populus alba L. et cinq lignées de P. tremula × Populus tremuloides Michx). La tolérance au glyphosate a été évaluée pendant 2 ans dans des dispositifs au champ établis dans l’est et l’ouest de l’Oregon. Dix pour cent des lignées transgéniques n’ont...
Tipo: Journal Article Palavras-chave: PLANTE TRANSGENIQUE; PEUPLIER HYBRIDE; GLYPHOSATE TRANSGENIC PLANT; HYBRID POPLAR; GLYPHOSATE.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007660e25ef&uri=/notices/prodinra1/2008/12/
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Transgenic poplar and the poplar leaf beetle: state-of-the-art on the risk of evolution of insect resistance Inra
Augustin, S.; Carré, G.; Courtin, C.; Dubois, V.; Genissel, A.; Lorme, P.; Wenes, A.L.; Rejasse, A.; Pilate, G.; Leplé, J.C.; Bourguet, D..
Tipo: Meeting Paper Palavras-chave: PEUPLIER TRANSGENIQUE; RESISTANCE AUX RAVAGEURS; ALLELE DE RESISTANCE; COUT DE LA RESISTANCE TRANSGENIC POPLAR; HARDWOOD; PEST RESISTANCE; RESISTANCE ALLELE; RESISTANCE COST; GMO.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007a544caaa&uri=/notices/prodinra1/2009/08/
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Wood formation in Angiosperms Inra
Dejardin, A.; Laurans, F.; Arnaud, D.; Breton, C.; Pilate, G.; Leplé, J.C..
La formation du bois est un processus de développement complexe, impliquant cinqétapes majeures : (1) la division des cellules à partir d’un méristème secondaire, appelé le cambium; (2) l’élongation des cellules ; (3) le dépôt d’une paroi secondaire épaisse ; (4) la mort programmée des cellules ; et (5) leur imprégnation par des composés phénoliques conduisant à la formation du bois de coeur. Grâce au développement des techniques de génomique et aux travaux menés sur la caractérisation d’arbres transgéniques, certains mécanismes moléculaires impliqués dans la formation du bois commencent à être mieux connus. Dans cette revue, deux aspects importants pour la qualité du bois seront approfondis : la lignification et le contrôle de l’angle des microfibrilles...
Tipo: Journal Article Palavras-chave: FORMATION DU BOIS; BOIS DE TENSION; PAROI SECONDAIRE; ANGLE DES MICROFIBRILLES WOOD FORMATION; TENSION WOOD; SECONDARY CELL WALL; MICROFIBRIL ANGLE; LIGNIN; POPLAR; GENOMICS.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20104db4dabe&uri=/notices/prodinra1/2010/05/
Registros recuperados: 16
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