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Registros recuperados: 4
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Expression of a poplar cDNA encoding a ferulate-5-hydroxylase/coniferaldehyde 5-hydroxylase increases S lignin deposition in Arabidopsis thaliana Inra
Sibout, R.; Baucher, M.; Gatineau, M.; Van Doorsselaere, J.; Mila, I.; Pollet, B.; Maba, B.; Pilate, G.; Lapierre, C.; Boerjan, W.; Jouanin, L..
Ferulate-5-hydroxylase or coniferaldehyde 5-hydroxylase (F5H or Cald5H; CYP84A1) is the enzyme responsible for the lasthydroxylation of the syringyl-type lignin precursors. A cDNA clone highly homologous to the Arabidopsis thaliana F5H/Cald5H cDNAwas identified during the random sequencing of a poplar (Populus trichocarpa) differentiating xylem cDNA library. Present as a multigenicfamily in poplar, this gene (PopF5H) was found to be highly expressed in lignified tissues supporting its role in lignin biosynthesis. Whenplaced under the control of the CaMV 35S promoter, the poplar F5H was able to complement the A. thaliana fah1-2 mutation.Overexpression in wild-type A. thaliana substantially increased the level of syringyl units in lignins. These transgenic...
Tipo: Journal Article Palavras-chave: BIOSYNTHESE DE LA LIGNINE; EXPRESSION DES GENES; ADNC FERULATE 5-HYDROXYLASE; POPULUS TRICHOCARPA; ARABIDOPSIS THALIANA.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007a66658c5&uri=/notices/prodinra1/2008/12/
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Identification of the structure and origin of thioacidolysis marker compounds for cinnamyl alcohol dehydrogenase deficiency in angiosperms Inra
Kim, H.; Ralph, J.; Lu, F.; Pilate, G.; Leplé, J.C.; Pollet, B.; Lapierre, C..
Molecular marker compounds, derived from lignin by the thioacidolysis degradative method, for cinnamyl alcohol dehydrogenase (CAD) deficiency in angiosperms have been structurally identified as indene derivatives. They are shown to derive from hydroxycinnamyl aldehydes that have undergone 8-O-4-cross-coupling during lignification. As such, they are valuable markers for ascertaining plant responses to various levels of CAD down-regulation. Their derivation illustrates that hydroxycinnamyl aldehydes incorporate into angiosperm lignins by endwise coupling reactions in much the same way as normal monolignols do, suggesting that the hydroxycinnamyl aldehydes should be considered authentic lignin precursors.
Tipo: Journal Article Palavras-chave: BIOSYNTHESE DE LA LIGNINE.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007f4cdd854&uri=/notices/prodinra1/2010/11/
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Quantitative 31P NMR spectroscopy of lignins from transgenic poplars Inra
Akim, L. G.; Argyropoulos, D. S.; Jouanin, L.; Leplé, J.C.; Pilate, G.; Pollet, B.; Lapierre, C..
Milled wood lignins isolated from genetically modified poplar trees were studied by quantitative 31P NMR spectroscopy, in combination with thioacidolysis and mild alkaline hydrolysis. The genetictransformation of the trees included down regulation of CAD and COMT enzyme activities.These analyses confirmed that moderate CAD down-regulation does not substantially alter ligninstructure. In contrast, severe CAD deficiency alters the lignin structure more profoundly by decreasingthe syringyl/guaiacyl ratio and increasing the degree of condensation of the lignin. Themost severe alterations were observed in the lignins from the COMT-transformed lines.
Tipo: Journal Article Palavras-chave: PEUPLIER TRANSGENIQUE; RMN; RESONANCE MAGNETIQUE NUCLEAIRE; THIOACIDOLYSE; HYDROLYSE ALCALINE TRANSGENIC POPLAR; NMR; NUCLEAR MAGNETIC RESONANCE; THIOACIDOLYSIS; ALKALINE HYDROLYSIS.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20071e4afa41&uri=/notices/prodinra1/2008/12/
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Secretome analysis of Phanerochaete chrysosporium strains CIRM-BRFM41 grown on softwood Inra
Ravalason, H.; Jan, G.; Mollé, D.; Pasco, M.; Coutinho, P.M.; Lapierre, C.; Pollet, B.; Bertaud, F.; Petit-Conil, M.; Grisel, S.; Sigoillot, J.C.; Asther, M.; Gimbert, I..
Abstract Proteomic analysis was performed to determine and differentiate the composition of the secretomes of Phanerochaete chrysosporium CIRM-BRFM41, a peroxidasehypersecretory strain grown under ligninolytic conditions and on softwood chips under biopulping conditions.Extracellular proteins from both cultures were analyzed by bidimensional gel electrophoresis and matrix-assisted laserdesorption/ionization time-of-flight tandem mass spectrometry.A total of 37 spots were identified. The secretome in liquid synthetic medium comprised mainly peroxidases,while several wood-degrading enzymes and enzymes involved in fungal metabolism were detected in biopulping cultures on softwood. This prompted an analysis of the impact of secretome modulation in the presence...
Tipo: Journal Article Palavras-chave:  SECRETOME SOFTWOOD CHEMICAL PULPING; Phanerochaete chrysosporium.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20092bd81e9f&uri=/notices/prodinra1/2010/09/
Registros recuperados: 4
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